As a result, freezing profiles are extremely consistent from one run to the next. Also, because of this low ther-
mal mass, the CoolCell SV10 container will not cause a rise in local freezer temperature and will protect nearby
samples already stored in the freezer. Low thermal mass also means CoolCell SV10 container will rapidly return
to room temperature for another freezing cycle (see fast recycle instructions above).
CoolCell SV10 freezing performance
The CoolCell SV10 container will freeze six serum vials
each containing 5ml of cell suspension at -1
o
C
per minute
when placed in a -8o
o
C
environment (mechanical freezer
or dry ice locker). The graph (left) displays a typical
freezing profile obtained using these conditions.
Note: “CC-12” refers to CoolCell 12-well 1ml cryovial
freezer, #BCS-136.
Troubleshooting
Care and Cleaning
The CoolCell SV10 container is constructed of closed cell crosslinked polyethylene foam and a solid thermo-
conductive core. CoolCell SV10 container is compatible with prolonged cryogenic temperature exposure. The
foam may be cleaned by water and mild soap. Rinse and dry thoroughly. CoolCell SV10 container is resistant to
alcohols and 10% bleach solutions. Do not autoclave. Maximum temperature exposure: 60
o
C
. Avoid prolonged
exposure to UV light sources.
As a result, freezing profiles are extremely consistent from one run to the next. Also, because of this low ther-
mal mass, the CoolCell SV10 container will not cause a rise in local freezer temperature and will protect nearby
samples already stored in the freezer. Low thermal mass also means CoolCell SV10 container will rapidly return
to room temperature for another freezing cycle (see fast recycle instructions above).
CoolCell SV10 freezing performance
The CoolCell SV10 container will freeze six serum vials
each containing 5ml of cell suspension at -1
o
C
per minute
when placed in a -8o
o
C
environment (mechanical freezer
or dry ice locker). The graph (left) displays a typical
freezing profile obtained using these conditions.
Note: “CC-12” refers to CoolCell 12-well 1ml cryovial
freezer, #BCS-136.
Troubleshooting
Care and Cleaning
The CoolCell SV10 container is constructed of closed cell crosslinked polyethylene foam and a solid thermo-
conductive core. CoolCell SV10 container is compatible with prolonged cryogenic temperature exposure. The
foam may be cleaned by water and mild soap. Rinse and dry thoroughly. CoolCell SV10 container is resistant to
alcohols and 10% bleach solutions. Do not autoclave. Maximum temperature exposure: 60
o
C
. Avoid prolonged
exposure to UV light sources.
Problem
Solution
Vials do not freely fit in the
chambers
The CoolCell SV10 container is designed to fit standard serum vial
shaped vessels up to 23mm in diameter and up to 47 mm in height.
Check that flag style labels, if used, will not bind and hinder insertion
or removal.
Vials are stuck in CoolCell SV10
after freezing
It is likely moisture was in the vial chambers or on the sample vial prior
to freezing. Remove the core (black ring) and tap the CoolCell SV10 to
dislodge vials.
The lid does not fully seat
Ensure that sample vials are properly seated. The maximum height of
the tube is 47mm.
Problem
Solution
Vials do not freely fit in the
chambers
The CoolCell SV10 container is designed to fit standard serum vial
shaped vessels up to 23mm in diameter and up to 47 mm in height.
Check that flag style labels, if used, will not bind and hinder insertion
or removal.
Vials are stuck in CoolCell SV10
after freezing
It is likely moisture was in the vial chambers or on the sample vial prior
to freezing. Remove the core (black ring) and tap the CoolCell SV10 to
dislodge vials.
The lid does not fully seat
Ensure that sample vials are properly seated. The maximum height of
the tube is 47mm.
15 Elizabeth Dr., Chelmsford, MA 01824 USA brookslifesciences.com/contact-us brookslifesciences.com
CoolCell SV10 Instructions, May 2018
CoolCell SV10 ©Brooks Automation, Inc. All rights reserved.
15 Elizabeth Dr., Chelmsford, MA 01824 USA brookslifesciences.com/contact-us brookslifesciences.com
CoolCell SV10 Instructions, May 2018
CoolCell SV10 ©Brooks Automation, Inc. All rights reserved.
