14
light falls on the object that is being observed.
From there, the light is reflected back and pass
-
es through the objective and eyepiece (where it
gets magnified) into the eye. This is reflected
light microscopy.
For transparent objections (e.g. protozoa), on
the other hand, the light shines from below,
through the opening in the microscope stage
and then through the object.
The light travels further through the objective
and eyepiece, where it is also magnified, and fi
-
nally goes into the eye. This is transmitted-light
microscopy.
Many microorganisms in water, many plan
components and the smallest animal parts
are already transparent in nature. Others have
to be prepared. We may make them transpar-
ent through a treatment or penetration with
the right materials (media), or by taking the
thinnest slices from them (using our hand or a
specimen slicer), and then examine them. You
can read more about this in the following sec-
tions.
How do I make thin specimen slices?
Only do this with the supervision of your par-
ents or another adult.
As I already pointed out, the thinnest slices
possible are taken from an object. In order to
get the best results, we need some wax or par-
affin. It is best if you get a candle. Place the
wax in a pot and heat it carefully over a low
burner. Now, dip the object in the liquid wax a
few times. Then, let the wax get hard. Using the
specimen slicer (23) or a knife/scalpel, cut the
smallest slices from the object that is covered
with wax. These slices are to be laid on a slide
and covered with a cover slip.
How do I make my own specimens?
Take the object that you want to observe and
place it on a glass slide (20). Then, add a few
drops of distilled water on the object (Fig. 7)
using a pipette (Fig. 7, 26). Now, place a cover
slip vertically at the edge of the drop of water,
so that the water runs along the edge of the
cover slip. Then, slowly lower the cover slip
over the water drops (Fig. 8).
Fig. 7
Fig. 8
2^
Note:
The included glue “gum media” (Fig. 5, 21b) is
used to make permanent prepared specimens.
Use this in place of the distilled water. If you
want to keep the object in place on the slide
permanently, use the gum media.
Experiments
Use the following web link to find interesting
experiments you can try out.
http://www.bresser.de/downloads
Microscope accessories can be found here:
http://www.bresser.de/P8859480
How do I install the MicrOcular?
Note:
The MicrOcular only works without the includ-
ed Barlow lens! The magnification setting is
changed when the MicrOcular is used, and it
must be readjusted with the focus knob.
First, remove the Barlow lens (Fig. 4, 3) with
the current eyepiece from the eyepiece support
(Fig. 4) and place the MicrOcular (Fig. 5, 4) in
the eyepiece (Fig. 5).
Fig. 4
D
f
Fig. 5
E
F
