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7.3  Performing an xxplate Sealing Operation 

A typical operating sequence to seal an 

xxplate

 is: 

1)  Switch the unit on and set the sealing time to 1.5 seconds and the 

heater plate set-point temperature to 170ºC (using the method 
described above). These are the recommended setting for the 

xxplate

 film. 

 

2)  If necessary, press the 

HEATER

 button to turn the heater on.  

 
3)  Allow sufficient time for the unit to reach temperature (the 

Heater

 

LED will stop flashing and remain constantly on). 

 

4)  Fit the 

BJS xxplate Adapter Block

 into the 

PCR 96 Well Skirted 

Plate

 

Adapter Block

 (both provided with the 

xxsealer

) and locate 

in the 

Adapter Block Carrier

 

 

 

 

 
5)  Load the 

xxplate

 onto the 

xxplate Adapter Block

 locating it onto 

the sprung pins. It should sit flat on the adapter block.  
And add the sealing film on top, the correct way up.  
 

 

WARNING 

Care should be taken not to touch 

the surface of the heater plate whilst 

loading the sample plate. 
 

 

XXSEALER USER MANUAL - 1.0 

 

 

 

Summary of Contents for xxsealer

Page 1: ...PCR plate heat sealer with programmable temperature and dwell User Manual This Manual Copyright 2014 BJS Biotechnologies ...

Page 2: ...s that may appear in this or related documentation Document XP 5003 01 1 Revision 1 0 Jan 2014 xxpress xxplates and xxsealer are the brand names for BJS Biotechnologies own range of PCR thermal cycler products and accessories For further details please visit our web site www xxpressPCR com All sales and technical enquiries should be addressed to BJS Biotechnologies Ltd 65 Bideford Avenue Perivale ...

Page 3: ... Operation 18 7 4 Switching Off the Audible Alert 20 8 Maintenance and Servicing 21 8 1 Replacing the Unit Fuse 21 8 2 Over Temperature Safety Cut out 22 8 3 Routine Cleaning and Inspection 22 8 4 Decontamination Procedure 23 8 5 Transportation and Storage 23 9 Warranty and Returns 25 10 Product Disposal 26 Tables Table 1 Advisory Symbol Meanings 5 Table 2 Unit Features 6 Table 3 Plate and Adaptor...

Page 4: ... featuring 24 48 96 or 384 wells MTP Microtitre plate PCR Polymerase Chain Reaction PPE Personal Protective Equipment SBS Society for Bio molecular Screening Semi skirted PCR Plate PCR plate with an outer surrounding half edge Skirted PCR Plate PCR plate with an outer surrounding edge Un skirted PCR Plate PCR plate without an outer surrounding edge Well xxplate A single sample cavity in a Microtit...

Page 5: ... which could if not avoided result in serve injury or death WARNING Indicates a hazardous situation which could if not avoided result in serve injury or death or severely damage the unit CAUTION Indicates a hazardous situation which could if not avoided result in minor or moderate injury or degrade or impair the functionality of the unit CAUTION Indicates a Risk of Crush hazard due to moving parts...

Page 6: ...sealing time and temperature and gives a visual countdown display during the sealing process for consistent results The unit has the following external features Table 2 Unit Features Seal Activation Handle 7 2 User Interface Display and Control Buttons 7 1 Heater Plate Internal 7 1 Adaptor Block Carrier Table 3 Rear Ventilation Holes 5 Mains Voltage Selector 5 Product Information Label 4 Power Inl...

Page 7: ...Types BJS xxplate Suitable for 24 54 and 96 well xxplates 96 Well Microtiter Plate 384 Well Microtiter Plate PCR 96 Well Skirted Plate PCR 96 Well Semi skirted Plate PCR 96 Well Un skirted Plate Deepwell Plates Plate Material Types Polypropylene Polyethylene or Polystyrene Other specialist adaptor plates may be available at request Please contact your distributor for details XXSEALER USER MANUAL 1...

Page 8: ...ear Polyester Polypropylene Laminate Clear Polymer Thin Clear Polymer Foils Foil Polypropylene Laminate Foil Laminate Foil Note The only film approved for use with xxplates is the BJS Bio s xxplate sealing film Part number HS 3001 01 1 XXSEALER USER MANUAL 1 0 ...

Page 9: ... C14 DPST Single Fuse 115 110 to 120 VAC 10 230 220 to 230 VAC 10 50 to 60 Hz 5 350 W max 115 T3 15AH 250V 20x5mm 230 T1 6AH 250V 20x5mm Operating Environment Temperature Range Relative Humidity Range Maximum Operating Altitude 18 to 30 C 20 to 80 non condensing 2200 m above sea level Storage and Transportation Temperature Range Relative Humidity Range 10 to 50 C 20 to 95 non condensing Sealing Pa...

Page 10: ...ecautions and warnings given in these instructions before attempting to operate the unit Improper use of this unit or its accessories may impair their functionality and invalidate the manufacturer s warranty Unit Handling Precautions CAUTION Care should be taken not to drop the unit or subject it to rough physical handling both during normal use and during transportation and storage WARNING The un...

Page 11: ...power cord provided with the unit or as specified Section 4 The unit must be connected to a suitably earthed mains supply with appropriate earth leakage and over current protection WARNING Always ensure that the mains power connector is securely inserted into the rear of the unit and any excess power cord does not pose a potential trip or pull hazard DANGER Do not operate the unit in any area whic...

Page 12: ...nsiderable time after being turned off Extreme care must be taken not to touch the heater plate as it will cause a serve burn injury DANGER The unit is intended for use with plates containing biological samples only Never use the unit to seal any explosive volatile or highly reactive substances or chemicals CAUTION There is a possible finger crush hazard due to the moving parts of the handle and p...

Page 13: ...tails DANGER Always switch off the unit and disconnect the power cord before performing any cleaning or decontamination procedure If liquid is spilt into or over the unit switch off and disconnect the power from the AC mains outlet before attempting to deal with the spillage CAUTION Ensure that the heater plate has cooled down to room temperature before performing any cleaning operation and before...

Page 14: ...ent see Section 5 Solid stable level working surface At least 10cm clearance around the unit to adjacent objects and walls Earthed AC mains power connection see Section 4 WARNING Please also observe and abide by the Unit Installation and Operating Environment safety precautions and preconditions listed in Section 5 It is important the check that the Mains Voltage Selector switch on the rear of the...

Page 15: ...uitably selected working surface as specified above ensuring that the ventilation holes on the rear of the unit are not obstructed 2 Connect the unit to the AC mains power outlet using the mains power cord supplied or as specified in Section 4 3 Switch the mains power on at supply outlet first and then switch the unit on using the power switch located at the rear of the unit 4 Check that the unit ...

Page 16: ...ts of a large 3 digit LED display four control buttons and three status indicator LEDs The LED display shows the current temperature or sealing time set point depending on the mode The button and LED functions are listed in Table 6 Table 6 User Buttons and Status LEDs Heater Status LED HEATER On Off Button Off Heater Turned Off Flashing Warming Up On Unit Ready For Use DOWN Button UP Button MODE S...

Page 17: ...ps Pressing the HEATER button toggles the plate heater between on and off When the Heater LED is flashing the heater is on and the plate is warming up which will normally take about 10 minutes Once the Heater is on the LED display shows the current temperature or sealing time set point depending on the mode When the heater plate is up to temperature within 2ºC of the set point the Heater LED will ...

Page 18: ...low sufficient time for the unit to reach temperature the Heater LED will stop flashing and remain constantly on 4 Fit the BJS xxplate Adapter Block into the PCR 96 Well Skirted Plate Adapter Block both provided with the xxsealer and locate in the Adapter Block Carrier 5 Load the xxplate onto the xxplate Adapter Block locating it onto the sprung pins It should sit flat on the adapter block And add...

Page 19: ...iately return the handle to its upmost position to retract the sealed sample plate from the heater plate CAUTION Failure to release the handle when sealing is complete could degrade the sample plate and seal quality 9 Remove the sealed plate from the unit and review the seal integrity WARNING The sample plate and film foil may remain hot for several seconds after being removed from the unit For op...

Page 20: ... necessary press the MODE button to select the Temperature LED setting mode 2 Next simultaneously press both the HEATER and MODE buttons together 3 The display should show 01 or 00 If it does not repeat this procedure from step 1 above 4 Now press the DOWN button to turn the alert off display shows 00 or the UP button to turn the alert back on display shows 01 5 Finally press the MODE button to st...

Page 21: ...echnician DANGER The unit fuse will only blow as a result of an internal unit fault or if the voltage selector switch has been incorrectly set see Section 5 This fuse should only be changed after the unit has been thoroughly inspected and must be replaced with the exact type specified in Section 4 Thoroughly inspect the unit for any signs of damage loose components or liquid spillage or ingress If...

Page 22: ...s or cleaning 2 Before cleaning always inspect the unit casework heater plate and moving parts for any signs of wear damage cracks or other defects 3 Clean the casework using a damp cloth soaked with a disinfectant solution such as Virkon whilst wearing suitable PPE 4 Carefully clean the heater plate surface to remove any debris or sealing material 5 Remove any debris or fluff from around or betwe...

Page 23: ... with suitable disinfectant solution such as Virkon 8 5 Transportation and Storage The xxsealer unit and its accessories should be thoroughly decontaminated using the procedure detailed in Section 8 4 before being placed in its original packaging for transportation or storage WARNING Refer to Section 4 for the acceptable range of Storage and Transportation environmental conditions Always ensure th...

Page 24: ...packaging etc with a damp cloth soaked with suitable disinfectant solution such as Virkon Allow to dry fully before packing 2 Decontamination Declaration Company Name Address Product Code xxsealer 953 A VTS Serial Number Reason For Return Where Product Used Please tick the appropriate option s below I certify that I have decontaminated the product as per the above procedure Decontaminant Used I ce...

Page 25: ...ed by BJS Biotechnologies will invalidate this warranty This warranty is provided to the original purchaser of the product for one year from the date of purchase Under the terms of this warranty the product must be returned in its original packaging transportation prepaid by the sender with a copy of the Proof of Purchase and a detailed description of the problem WARNING The product must be decont...

Page 26: ...c equipment The unit and its accessories should be decontaminated using the procedure detailed in Section 8 4 before disposal or shipping Do not dispose of this product into unsorted municipal waste or public landfill Please contact your distributor or BJS Biotechnologies at the address on Page 2 of this manual for details of how to correctly dispose of this product XXSEALER USER MANUAL 1 0 ...

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