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V1.6.3
DNA Quality Problems
Problem
Cause
Solution
Low DNA yield
Incomplete lysis
Decrease the amount of starting material used.
Be sure to add Proteinase K during lysis, if included in
the protocol.
Make sure that the sample is completely immersed in
the Lysis Buffer.
Poor quality of starting material
Be sure to process sample immediately after collection
or store the sample at appropriate temperature. The
yield and the quality of DNA isolated depend on the
starting material.
Insufficient amount of magnetic
beads added
During shipping, some magnetic bead solution may ad-
here to the sealing foil of the cartridge. To collect any
bead solution from the foil, tap the cartridge to deposit
the bead solution at the bottom of the well.
Clogged Tips resulting in DNA loss
Ensure that the lysate does not contain any particulate
material that can clog the tip sprout. If needed, centri-
fuge the sample prior to the BioMagPure purification.
No DNA recovered
Magnetic beads stored or handled
improperly
Store cartridge containing the beads at room tempera-
ture.
Do not freeze the cartridge as the beads may be irrepa-
rably damaged.
Make sure that the beads are in solution at all times and
do not dry. Dried beads are non-functional.
Elute containing DNA
is discoloured
Magnetic beads present in the elute
Remove any magnetic beads using a magnetic separa-
tor or centrifuge the sample in a micro centrifuge for 1
minute at maximum speed.
DNA contaminated with heme
Minimize the amount of blood or bloodstained sample
used (≤ 20μl blood spot for forensics sample).
DNA is sheared or de-
graded
Bubbles formed during mixing steps
To prevent bubble formation during mixing, make sure
the sample volume is at least the recommended volume
listed in the manual supplied with Reagent Kits.
Purified DNA repeatedly frozen and
thawed
Aliquot purified DNA and store at 4°C (short-term) or -
20°C (long-term). Avoid repeated freezing and thawing.
DNA contaminated with DNases
Maintain a sterile environment while working (i.e. wear
gloves and use Dnase-free reagents).
Summary of Contents for BioMagPure 12 Plus
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