TG
RADIENT
Manual __________________________________________________________ 21
Note:
The time increment will have an impact on the total runtime depending on the numbers of
cycles and the size of the increment. A program with many cycles and large time increments will
take a significantly longer time than a standard protocol.
6.2
Program touch down
For some applications it is useful to start with a higher annealing temperature and to decrease the
temperature from cycle to cycle. This subsequent lowering of the annealing temperature is called
touch down.
To decrease the annealing temperature from cycle to cycle enter a negative temperature
increment in the row labeled with dT[s].
Main funct.
dT[°C]
dt[s]
ä
[°/s]
2:
ß
5.00
3:
ß
5.00
4:
ß
-0.20
5.00
5:
ß
5.00
A ?
B header
C pgm OK
D enter
Note:
Be sure that the temperature decrease is set in a step that lies within a loop. Otherwise
there will be no iterative temperature decrease.
6.3
Adjust heating and cooling ramps
Since the TG
RADIENT
is a extreme fast thermocycler it may be necessary to reduce the heating
and cooling ramp for some applications. For example this is helpful if cycling protocols from
other cyclers shall be used.
The heating or cooling ramp respectively can be set in the row labeled with
ä
[°/s].
Main funct.
dT[°C]
dt[s]
ä
[°/s]
2:
ß
5.00
3:
ß
5.00
4:
ß
5.00
5:
ß
5.00
A ?
B header
C pgm OK
D enter
Note:
The default is [5.00 °/s] which means maximum ramping speed.