Bioline ISOLATE II Plant DNA Kit, Product Manual

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Plant DNA Kit
5
3. STORAGE
Store lyophilized RNase at 4°C on arrival (stable for up to 1 year). Reconstituted RNase I
solution is stable for 12 months at ‑20°C. All other kit components should be stored at room
temperature (18‑25°C) and are stable for at least one year. Storage at lower temperatures
may cause precipitation of salts.
4. SAFETY INFORMATION
When working with chemicals, always wear a suitable lab coat, gloves and safety glasses.
Buffers PA1, PA2, PB and PAW1 contain guanidine hydrochloride and/or detergents like
CTAB or SDS.
For detailed information, please consult the material data safety sheets (MSDSs) available on
our website at www.bioline.com.
5. PRODUCT SPECIFICATIONS
The ISOLATE II Plant DNA Kit is specially designed for the rapid and efficient isolation of
extremely pure genomic DNA from plant cells and tissues or filamentous fungi. The DNA
is suitable for a wide variety of applications e.g. real‑time PCR and DNA sequencing. The
preparation time is 30 min for 6 preps. The isolated DNA is of high‑purity, yield and is free
from enzymatic inhibitors (see below).
ISOLATE II PLANT DNA SPIN COLUMN SPECIFICATIONS
Max. binding capacity 50 μg DNA
A
260
/A
280
ratio* 1.6–1.9
Typical yield* 1‑30 μg
Elution volume 2 x 50 μL
Max. amount of starting material
Plant tissues
Filamentous fungi
100 mg (wet)/20 mg (dry)
100 mg (wet)/20 mg (dry)
* DNA yields vary between different species and tissues depending on genome size, ploidy,
cell number and sample age.
The following components are also included in the kit:
•
ISOLATE II Filters for homogenization and to clear crude lysates.
• RNase A to eliminate RNA and to facilitate quantification of genomic DNA by photometry.
• Two highly optimized lysis buffers, PA1 and PA2 containing CTAB and SDS detergents,
respectively.