Bioline ISOLATE II Plant DNA Kit, Product Manual

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Product Manual www.bioline.com/isolate
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1. KIT CONTENTS
2. DESCRIPTION
The ISOLATE II Plant DNA Kit is a simple, reliable and fast method for isolation of high‑quality
genomic DNA from plant cells and tissues as well as filamentous fungi. Plant samples are
first disrupted/homogenized and then lysed in a highly optimized buffer system, containing
chaotropic salt, denaturing agent and detergents. A choice of two lysis buffers based on the
established CTAB or SDS methods are provided. Crude lysates are cleared by centrifugation
and/or filtration using the ISOLATE II Filters to remove polysaccharides, insoluble plant debris
and other contaminants. The cleared lysate is then mixed with the Binding Buffer and processed
through an ISOLATE II Plant DNA Spin Column containing a silica membrane to which the
plant genomic DNA binds. Contaminants and impurities such as salts, metabolites and cellular
components are removed by simple washing steps with two different buffers. High‑quality
purified plant genomic DNA is then eluted in a low salt Elution Buffer or nuclease‑free water.
The DNA is ready‑to‑use for a wide variety of applications e.g. real‑time PCR, next generation
sequencing, restriction analysis, and array technology.
Please read this manual carefully to familiarize yourself with the ISOLATE II Plant DNA
protocol before starting (also available on www.bioline.com). More experienced users can
refer to the bench‑top protocol for quick referencing during the procedure.
COMPONENT 10 Preps 50 Preps 250 Preps
ISOLATE II Filters (violet) 10 50 250
ISOLATE II Plant DNA Spin Columns
(green)
10 50 250
Collection Tubes (2 mL) 20 100 500
Lysis Buffer PA1 5 mL 25 mL 125 mL
Lysis Buffer PA2 4 mL 20 mL 100 mL
Precipitation Buffer PL3 1 mL 10 mL 25 mL
Binding Buffer PB 6 mL 30 mL 125 mL
Wash Buffer PAW1 6 mL 30 mL 125 mL
Wash Buffer PAW2
†
(concentrate) 6 mL 25 mL 50 mL
Elution Buffer PG 13 mL 13 mL 30 mL
RNase A (lyophilized) 1.5 mg 6 mg 2 x 15 mg
Product Manual 1 1 1
Bench Protocol Sheet 1 1 1
†
Before use, add indicated volume of 96‑100% ethanol and mark wash buffer bottle label.