BIOLABO Diagnostics KENZA 240 TX, User Manual, Page: 13 / 56

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8) Press “Exit” to return to the main menu. Press the “CK Cuvettes” key and then the “Enter” key.
The cuvettes will first be filled with approximately 700 µl of distilled water, after which each will undergo a light
beam reading, representing zero or the starting point for analytical measurement.
On completion, the following table will be shown with readings performed on 50 cuvettes of various wavelengths.
•
Line 01 shows the reading in volts
performed on cuvette 1
•
Differences between cuvette 1
and the other 49 are shown from
line 02 to line 50.
In other terms, the system measures
transmitted light, or transmittance, via
cuvette 1 at different wavelengths and
writes the values in line 01 of the table. It
then performs this same operation for
cuvette 2, subtracting the transmittance
values from those of cuvette 1 and then
bringing them to line 02 of the table. This
process is repeated for all cuvettes for
varying wavelengths.
The symbol “-” indicates that the subtraction of transmittance can be positive or negative.
The Offset indicates the value measured in the absence of light (in this case, the filters wheel will select a black
window). This check, beside setting the zero or starting point of photometric reading, is useful for verifying the
status of the cuvettes.
Voltage values between 3.5-8.5 volts are acceptable for cuvette 01 at various wavelengths. The Offset for the
recommended range is 0.002-0.006.