Gene Explorer Thermal Cyeler Operation Manual
Chapter Four Operation Instructions
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2.4 Extended parameter
On the interface of PCR program setting, click “
Extended parameter
” and it will
prompt a box for setting extended temperature, extended time, speed, and gradient and other
operations.
Extended temperature: the volume of temperature increase or decrease for each loop.
“-/+” changes the positive and negative of data.
Extended time: time increase for each loop.
Speed: ramp rate
Ladder Temp: It refers to the temperature difference between two adjacent modules .The
range is -6
℃
~6
℃
. “Temperature” is set to the temperature of the leftmost module.
2.5 Save experiment
On the interface of PCR program setting, click “Save”, and input user name and file
name to save the file. Files saved can be found under “Open Experiment”.
3 Run program
Before running experiment, set up hot lid temperature, reaction volume, temperature
control mode and control mode on the interface of PCR program setting and then click “
Run
”
to start the experiment. You can also run experiment by directly selecting the file under
“
Open Experiment
” and “
Recently Opened
”.
