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biochrom Anthos Fluido 2, User Manual

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Biochrom Anthos Fluido-2 Microplate Washer v2.0 

 

Page 1 

 

 

Biochrom Anthos Fluido 2 

    Microplate Washer 

  User

’s Manual 

 

 

 

 
 
 
 

 
 
 
 

Biochrom Ltd 

 

22 Cambridge Science Park,  
Milton Rd, Cambridge CB4 0FJ UK 

Tel: +44 (0)1223 423723 
Fax: +44 (0)1223 420164 
Email: [email protected] 
www.biochrom.co.uk 
 
 
 
 
 

 
 
 

Biochrom Ltd 

 

22 Cambridge Science Park,  
Milton Rd, Cambridge CB4 0FJ UK 

Tel: +44 (0)1223 423723 
Fax: +44 (0)1223 420164 
Email: [email protected] 
www.biochrom.co.uk 
 
 
 
 
 

 
 
 
 

Summary of Contents for Anthos Fluido 2

Page 1: ...om Ltd 22 Cambridge Science Park Milton Rd Cambridge CB4 0FJ UK Tel 44 0 1223 423723 Fax 44 0 1223 420164 Email enquiries biochrom co uk www biochrom co uk Biochrom Ltd 22 Cambridge Science Park Milto...

Page 2: ...Standard delivery content 12 4 6 Warnings and directions 13 4 7 Unpacking Procedure 14 4 8 Installation requirements Environmental operating conditions 14 4 9 Instrument installation procedure 15 4 1...

Page 3: ...4 Define Cycle Menu 47 9 5 Define Plate Menu 52 9 6 Setup Menu 65 9 7 Service Menu 69 10 ERROR MESSAGES 70 10 1 1 Duplicate Name 70 10 1 2 Wrong Manifold 70 10 1 3 No Cycle Defined 70 10 1 4 No Plate...

Page 4: ...tes state of the art technology and software both necessary to allow optimum performance and easy use Easy operation through the menu structure of the on board software The display and the keyboard pr...

Page 5: ...disconnect the device from the power source Ensure that the power cord supplied with the unit is used The power cord may only be inserted in a socket outlet provided with a protective ground earth co...

Page 6: ...floppy disks audio or video tapes near to the magnet of the plate carriage risk of data loss Be careful when placing the plate carriage onto other sensitive medical devices which may be influenced by...

Page 7: ...Washer v2 0 Page 7 The symbol on the product indicates that this product shall not be treated as household waste Instead it shall be handed over to the applicable collection point for the recycling of...

Page 8: ...quid does not attack the surface of the housing Regular cleaning The housing of the instrument should be cleaned regularly with a mild household cleaning agent Warning Do not use aggressive solutions...

Page 9: ...ys Ambient temperature 15 C 40 C operation 25 C 50 C storage Relative humidity 15 85 non condensing operation 95 non condensing storage Air pressure tolerance 54 000 106 000 Pascal Maximum altitude op...

Page 10: ...id spillage If any liquid is spilled on the instrument it should be IMMEDIATELY removed so that the liquid does not attack the surface of the housing Regular cleaning The housing of the instrument sho...

Page 11: ...can be used to hold the wash buffer No special bottles are required Quiet operation The Fluido 2 is very quiet due to the special pumps used Additionally the pumps are only operating when the washing...

Page 12: ...rip by strip STRIP mode The number of strips to be processed may be defined at the time the wash procedure is started Options Liquid level detectors to check volumes in wash and waste bottles Remote c...

Page 13: ...nclosed with such substances Never spill fluids in or on the equipment Wash your hands thoroughly after handling test fluids If the equipment has been in contact with hazardous substances it must be d...

Page 14: ...ng note and packing list 7 Please save all packing materials as they maybe required for later transportation 4 8 Installation requirements Environmental operating conditions Flat dry clean and vibrati...

Page 15: ...Afterwards slide the dispense tube on the blue connector of the manifold Place the manifold now on the horizontal manifold holder bar so that the two holding pins are resting in the slots of the mani...

Page 16: ...n Not illuminated Instrument is not ready for operation Please Note The power indicator does not indicate that the instrument is turned on or off or that it is connected with mains but only that the i...

Page 17: ...n picture 1 3 Put the transport lock plate at the location shown in picture 2 and secure the plate with adhesive tape 4 Place the foam parts left and right on the washer and put the washer into the tr...

Page 18: ...alve and the manifold The input selection valves are connecting the respective liquid reservoirs wash and rinse solution to the internal dispense circuits The valves are controlled by the washer softw...

Page 19: ...Biochrom Anthos Fluido 2 Microplate Washer v2 0 Page 19 Diagram 1 Hydraulic Diagram...

Page 20: ...ing return transport due to wrong packing e g not in original packing material The Fluido 2 was damaged due to wrong operation not according to the descriptions in the manual The instrument may only b...

Page 21: ...tion key 2 F2 When pressing F1 or F2 the next respectively the previous menu will be displayed The two function keys may have different functions assigned in the various sub menus The current assignme...

Page 22: ...Biochrom Anthos Fluido 2 Microplate Washer v2 0 Page 22 By pressing enter the current selection is confirmed and the software leads to the next menu to allow further selections...

Page 23: ...m e g bottom wash and plates defines the shape and measures of the plate used are necessary Drawing 3 Software Principle Note Please make sure to program the cycle and plate BEFORE the procedure 7 3 M...

Page 24: ...to chapter 9 3 Define Procedure Menu For details please refer to chapter 9 4 Define Cycle Menu For details please refer to chapter 9 5 Define Plate Menu For details please refer to chapter 9 6 Setup...

Page 25: ...Run Procedure Run Procedure Washliquid OK Waste empty Plate inserted Yes Yes Perform Procedure Yes No No No Nr of strips Esc if disabled if disabled if disabled if Ask strip nr No Stop Table 4 Run Pr...

Page 26: ...ge 26 7 3 2 Prime Rinse Prime Rinse Manual Automat Period Liquid Liquid Volume Volume Rinse interval Manual prime rinse Esc Automatic Rinse Periodic Rinse Esc Esc Esc Liquid Esc Esc Esc Stop Table 5 P...

Page 27: ...asp power Processing m ode Plate Ask strip nr D elay final asp C ycle Storing Final asp tim e if Processing m ode Strip AN D Final asp tim e 0 Esc Esc Esc Esc Esc Esc Esc Liquid 1 8 C ycles Esc if Fi...

Page 28: ...Aspir tim e Aspir power Shaking tim e Shaking intensity Minim um soak tim e Disp volum e Disp power Storing if Disp volum e 0 if Aspir tim e 0 if Shaking tim e 0 W ash m ode Esc Esc Esc Esc Esc Esc E...

Page 29: ...ate form at W ell shape Side pos front Side pos rear Center pos Aspiration Ht Disp pos Bottom disp ht Crosswise asp Storing If Cross wise asp No Flat Round Esc Esc Esc Esc Esc Esc Esc Esc Esc Esc Ovfl...

Page 30: ...ids W ast full det Rinse em pty det W ash1 em pty det W ash2 em pty det Storing Esc Esc Esc Esc Esc Esc Esc W ash3 em pty det Liquid warning W aste warning Plate warning Key click Esc Esc Esc Esc Esc...

Page 31: ...sher v2 0 Page 31 7 3 7 Service Service Sensors Valves Pumps PlatInit PlatPos MFInit Esc Esc Esc Esc Esc Esc MFPos MFAdjust PlAdjust Eeprom DelParam Esc Esc Esc Esc Esc Table 10 Service For details pl...

Page 32: ...ending on the menu other functions can be assigned to these keys The current function is always shown in the display right above the key These keys are used for numerical or text input and to select o...

Page 33: ...2 performs the complete initialization and shows the main menu after approx 10 seconds Display Screen 11 Display Description The first line of the display shows the name of the menu or the current fu...

Page 34: ...2 0 Page 34 Screen 12 Setup Menu Please make sure to set all options according to your requirements before programming new procedures plates or cycles and before running procedures For details on this...

Page 35: ...F1 or F2 Select another menu or Select a procedure to be started enter Confirm the selection of the procedure and go to the next screen Screen 14 Wash liquid Check A warning to check the liquid level...

Page 36: ...her a plate is inserted is displayed this function needs to be activated in the Setup Menu Function keys F1 or F2 Select Yes or No If No is selected the instrument will return to the main menu Warning...

Page 37: ...croplate Washer v2 0 Page 37 enter Confirms the selection and starts procedure After confirmation of the above warnings the selected procedure will be started During the run the display will show the...

Page 38: ...prime rinse Available options are manual automatic periodic enter Confirm the selection of the prime rinse mode and go to the next screen 9 2 1 Manual Prime Rinse Screen 19 Liquid Selection Select th...

Page 39: ...u or Go to start the prime rinse keep this key pressed as long as you like the prime rinse to be performed 9 2 2 Automatic Prime Rinse Screen 21 Liquid Selection Select the liquid to be used for prime...

Page 40: ...en 23 Start Prime Rinse Function keys F1 or F2 Select Esc to return to the main menu enter Press enter to start the prime rinse cycle with the above set parameters Screen 24 Status Message Function ke...

Page 41: ...ys F1 or F2 Select Esc to return to the main menu or Select the desired volume in 1ml steps max 200ml enter Confirm the selection of the liquid volume and go to the next screen Screen 27 Rinse Period...

Page 42: ...t Stop to cancel the current prime rinse cycle and return to the main menu 9 3 Define Procedure Menu Screen 30 Define Procedure Menu In this menu a procedure can be defined Every procedure contains on...

Page 43: ...return to the main menu without saving any changes or Sel to select the current letter number or Use these keys to scroll through the available letters A Z and numbers 0 9 enter Confirm the name of th...

Page 44: ...h the available cycles enter Confirm the selection of the first cycle and go to the next screen Screen 35 Cycle2 8 Selection Select the cycles 2 to 8 to be used for this procedure The cycles must be d...

Page 45: ...screen Screen 37 Final Aspiration Power Entry Select the power for the final aspiration Function keys F1 or F2 Select Esc to return to the main menu without saving any changes or to return to the prev...

Page 46: ...scroll through the available plates enter Confirm the selection of the plate and go to the next screen Screen 40 Ask for Last Strip Select Yes if the selection of a number of strips should be possibl...

Page 47: ...the previous screen or Use these keys to toggle between Yes and No If Yes is selected the final aspiration is performed after the last cycle for all strips is finished enter Confirm the selection The...

Page 48: ...ys to scroll through the available letters A Z and numbers 0 9 enter Confirm the name of the cycle and go to the next screen Screen 45 Aspiration Time Entry Function keys F1 or F2 Select Esc to return...

Page 49: ...um High enter Confirm the selection of the aspiration power and go to the next screen Screen 47 Dispense Volume Entry Function keys F1 or F2 Select Esc to return to the main menu without saving any ch...

Page 50: ...ed position for the used plate during the wash procedure This means that basically only the bottom of the well is washed Overflow the needles of the manifold are placed to a certain position above the...

Page 51: ...u without saving any changes or to return to the previous screen or Use these keys to change the intensity for shaking Low Medium High enter Confirm the selection of the shaking intensity and go to th...

Page 52: ...set parameters will be stored and the program returns to the main menu 9 5 Define Plate Menu The following drawings help to find the correct positions of the manifold and plate Please note that curved...

Page 53: ...ttom and curved bottom plates Vertical 0 Position Aspiration ht Side pos rear Side pos front Vertical 0 Position Horizontal 0 Position Horizontal 0 Position Center pos Aspiration ht Dispense needle As...

Page 54: ...een 56 Plate Name Entry The name of the plate may be changed Function keys F1 or F2 Select Esc to return to main menu without saving any changes Select Chg to change the name for the plate enter Confi...

Page 55: ...F2 Select Esc to return to the main menu without saving any changes or to return to the previous screen or Use these keys to toggle between flat and round bottom plates enter Confirm the selection of...

Page 56: ...ttom of the well changes to Select to move the manifold up Select Mov to move the plate transport to the current center position or Use these keys to enter the center position The plate can be moved i...

Page 57: ...Biochrom Anthos Fluido 2 Microplate Washer v2 0 Page 57 aspiration is advisable Crosswise aspiration can only be used for flat bottom plates...

Page 58: ...position the needle close to one wall The side position front may be defined by teach in move the plate to the correct position with the and keys Function keys F1 or F2 Select Esc to return to the mai...

Page 59: ...on The side position rear may be defined by teach in move the plate to the correct position with the and keys Function keys F1 or F2 Select Esc to return to the main menu without saving any changes Se...

Page 60: ...Biochrom Anthos Fluido 2 Microplate Washer v2 0 Page 60 Select to move the manifold up Select Mov to move the plate transport to the current side position rear...

Page 61: ...Select Mov to position the manifold to the current aspiration height and switch to the teach in mode or Use these keys to enter the aspiration height in steps of 0 1mm range 0 18 8mm enter Confirm the...

Page 62: ...ach in move the plate to the correct position with the and keys Function keys F1 or F2 Select Esc to return to the main menu without saving any changes Select Mov to position the plate to the current...

Page 63: ...h cycle The dispensing height must be higher or equal the aspiration height It may be defined by teach in move the manifold to the correct position with the and keys Function keys F1 or F2 Select Esc...

Page 64: ...t be higher or equal as the aspiration height It may be defined by teach in move the manifold to the correct position with the and keys Function keys F1 or F2 Select Esc to return to the main menu wit...

Page 65: ...the program returns to the main menu 9 6 Setup Menu Please make sure to set all options according to your requirements Screen 75 Setup Menu Use the F1 F2 keys to select the setup menu Confirm the sel...

Page 66: ...0 Page 66 Screen 78 Liquids Setup Use the keys to select the number of wash liquids connected to your instrument and confirm with enter Depending on the hardware configuration of your instrument you c...

Page 67: ...tector must be connected to the instrument and the rinse bottle If activated the liquid level of the rinse bottle for a started procedure will be checked before the procedure is started In case that t...

Page 68: ...ys to select whether a liquid level sensor is attached to the wash3 bottle and confirm with enter To use this function a liquid level detector must be connected to the instrument and the wash3 bottle...

Page 69: ...activate the keyclick and confirm with enter Screen 88 Emergencystop Setup Use the keys to activate deactivate the emergencystop and confirm with enter If a strip of a microplate or the microplate its...

Page 70: ...r Confirm the message 10 1 2 Wrong Manifold Screen 91 Wrong Manifold The wrong manifold for the selected procedure is installed Function keys enter Confirm the message 10 1 3 No Cycle Defined Screen 9...

Page 71: ...rm the message 10 1 5 Waste Full Screen 94 Waste Full The waste is full Empty the waste bottle Function keys enter Confirm the message 10 1 6 Liquid Empty Screen 95 Liquid Empty The selected wash liqu...

Page 72: ...the message 10 1 8 Plate Transport Error Screen 97 Plate Transport Error The plate transport failed Function keys enter Confirm the message 10 1 9 Manifold Transport Error Screen 98 Manifold Transport...

Page 73: ...hos or an authorized Anthos Distributor may be installed in or used with Anthos Fluido 2 Fuses as specified in chapter 4 1 1 Rated operating conditions Power cable CE marked power cable with connected...

Page 74: ...ions The area surrounding the plate carrier should be cleaned with a dry cloth Fittings and tubing The usual wash buffer tends to crystallize easily on air and can block tubing and fittings then Use t...

Page 75: ...t 24 hours 7 After the standing time remove the instrument from the plastic bag and wipe all the outside surfaces of the instrument and the plate support area with a wad of cotton wool which has been...

Page 76: ...he concentration of the sample shall be diluted until no diagnostically relevant rest remains The main field of use is ELISA testing operated and carried out by trained specialist stuff medical and or...

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