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BIO RAD Trans-Blot Turbo, Instruction Manual

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iv     Trans-Blot® Turbo™ Blotting System

Summary of Contents for Trans-Blot Turbo

Page 1: ...Trans Blot Turbo Blotting System Instruction Manual Catalog number 170 4155...

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Page 3: ...this publication may be reproduced or transmitted in any form or by any means electronic or mechanical including photocopy recording or any information storage or retrieval system without permission...

Page 4: ...ii Trans Blot Turbo Blotting System...

Page 5: ...ranties and can put you at risk for electric shock Return the Trans Blot Turbo instrument or cassettes to the factory US customers or to an authorized distributor all other customers if repairs are ne...

Page 6: ...iv Trans Blot Turbo Blotting System...

Page 7: ...o operate the equipment This Bio Rad instrument is designed and certified to meet EN61010 and the EMC requirements of EN61326 for class A safety standards Certified products are safe to use when opera...

Page 8: ...vi Trans Blot Turbo Blotting System...

Page 9: ...ity noncondensing User Interface 18 button keypad 128 x 64 pixel monochrome display Programmable methods Up to 25 user defined Preprogrammed methods Standard SD 1 5 mm gels High MW Low MW Mixed MW 1 M...

Page 10: ...polyethylene terephthalate glycol PETG and can be recycled according to local regulations for class 1 plastics Trans Blot Turbo RTA Transfer Kits Membrane dimensions L x W Mini format 7 0 x 8 5 cm mi...

Page 11: ...ansfer Setup 6 3 1 Transfer Using Transfer Packs 6 3 2 Placing a Transfer Pack into the Cassette 8 3 3 Transfer Using RTA Transfer kits 10 3 4 Transfer Using the Turbo Protocol 11 3 4 a Disassembling...

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Page 13: ...t to access the power supply for protein transfer A preprogrammed user defined transfer protocol is easily selected from the firmware menus using the Navigation and Selection buttons and the alphanume...

Page 14: ...or operation at 15 31 C ambient temperature and 0 95 relative humidity noncondensing Operating the unit outside these conditions is not recommended To ensure adequate cooling be sure that there is at...

Page 15: ...ries that are not EN 61010 certified even when used with this unit This instrument should not be modified or altered in any way Alteration of this instrument will void the manufacturer s warranty void...

Page 16: ...consumables can also be used with the system See Section 3 7 page 15 for details For transfer a gel is sandwiched between two ion reservoir stacks in the cassette Each cassette can hold 1 or 2 mini f...

Page 17: ...use 2 1 User Interface The user interface consists of an LCD menu screen on the front of the system Three buttons below the screen are used to navigate among menu screens Three buttons to the right of...

Page 18: ...tin 2895 3 1 Transfer Using Transfer Packs Recommendations Wear gloves at all times during the blotting process to prevent contamination of the gels or membranes Transfer gels immediately after electr...

Page 19: ...a tab on the right side and is for the anode below the gel This stack is layered with the transfer membrane The ion reservoir stack for the cathode above the gel is located below the membrane and ano...

Page 20: ...tack must be moved after placement use a blot roller to expel any trapped air bubbles Each stack uses multiple layers of material for maximum transfer efficiency Removing stack layers is not advised R...

Page 21: ...tly use the blot roller to remove air bubbles between the gel and membrane If transferring two mini gels place them on the membrane so that the feet of the gels are facing toward each other c Gently p...

Page 22: ...els on a midi stack Set the transfer stack close to the center of the cassette A midi stack only fits along the long axis of the cassette It is not necessary to move an assembled transfer pack if it i...

Page 23: ...e of gel to be transferred Use the corresponding selection button to choose the option for the combination of gels in the run The option varies the current for the run 1 3 A for a single mini format g...

Page 24: ...gels that can be used in a single run and proceed to the Home menu to select the correct protocol 2 Unlock the cassette by turning the dial counterclockwise to the Unlock position 3 Disassemble the b...

Page 25: ...thick gels HIGH MW is optimized for more efficient transfer of large proteins 150 kDa LOW MW is optimized for more efficient transfer of small proteins 30 kDa MIXED MW is for efficient transfer of pr...

Page 26: ...uent use if desired A saved protocol can later be accessed under LIST USER DEFINED protocols Saved protocols can be deleted when no longer required Fig 18 Edit NEW protocol screen Selection buttons fo...

Page 27: ...the electrical contacts fit closely into the slots in the base Press the lid down firmly and turn the dial clockwise to engage the lid pins into the locking slots 6 Load a second cassette if desired...

Page 28: ...power conditions for most effecient transfers Use the blot roller to remove any air bubbles when assembling the transfer sandwich Air bubbles between layers of the assembled sandwich will prevent prot...

Page 29: ...ch contact to ensure that they all depress freely The contacts should be checked and cleaned monthly as part of standard equipment maintenance procedures Clean any accidental buffer spills in the inst...

Page 30: ...The detection system is not working or is not sensitive enough Include proper antigen controls to test detection kit sensitivity or use a total protein stain or Criterion Stain Free system to detect p...

Page 31: ...onsult your electrophoresis manual for more details When using a single mini transfer pack the assembled transfer pack may have been placed at the extreme edge of the cassette bottom Ensure that assem...

Page 32: ...Overview of the Three Protocol Modes TURBO 1 mini format or 2 mini format gels or 1 midi format gel in 7 min 1 Mini PROTEAN TGX gel in 3 min 20 Trans Blot Turbo Blotting System...

Page 33: ...LIST Bio Rad preprogrammed protocols or user defined protocols Trans Blot Turbo Blotting System 21...

Page 34: ...NEW User can edit protocol parameters and name save and run the protocol 22 Trans Blot Turbo Blotting System...

Page 35: ...Blot Turbo RTA Tranfer Kit PVDF midi format 13 5 x 8 5cm for 40 blotsl 170 4275 Trans Blot Turbo RTA Tranfer Kit LF PVDF mini format 7 0 x 8 5 cm for 40 blots 170 4276 Trans Blot Turbo RTA Tranfer Kit...

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Page 40: ...many 089 31 884 0 Greece 30 210 9532 220 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91124 4029300 Israel 03 963 6050 Italy 39 02 216091 Japan 81 3 6361 7000 Korea 82 2 3473 4460 Mexico 52 555...

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