BIO RAD Trans-Blot Turbo, Instruction Manual

Page: 17 / 40

Acceptable Gel Combinations Unacceptable Gel Combinations
1 2 1 2
Upper bay A 1 mini format gel 2 mini or 1 midi
format gels
1 mini format gel 2 mini or 1 midi
format gels
Lower bay B
1 mini format gel 2 mini or 1 midi
format gels
2 mini or 1 midi
format gels
1 mini format gel
The cassette bays are labeled A and B for convenience, and the cassettes are freely interchangeable
between the bays. There is no preference as to which bay contains which cassette when running the
combination of 1 midi and 2 mini gels. Also, one bay (either A or B) can remain empty during a run.
Table 1. Acceptable gel transfer combinations when both bays are in use.
2.1 User Interface
The user interface consists of an LCD menu screen on the front of the system. Three buttons below the
screen are used to navigate among menu screens. Three buttons to the right of the screen are for user
selections. A standard alphanumeric keypad is used for input of text and numbers.
From the Home menu, three modes are available:
• NEW mode. Create, run, and save a new protocol for your protein of interest
• LIST mode. Select from either a list of Bio-Rad preprogrammed protocols optimized for a variety of
protein and gel types or a list of user-defined protocols
• TURBO mode. Quick access menu for transfers of mixed MW proteins (MW 5–150 kD). Designed for
efficient transfer of a wide variety of proteins over a broad range of molecular weights
Fig. 4. Trans-Blot Turbo system user interface.
Trans-Blot® Turbo™ Blotting System 5