BIO RAD Trans-Blot Turbo, Instruction Manual

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Fig. 17. User protocols screen.
3.6 Transfer Using a New Protocol
Assembly of the blotting sandwich is described in sections 3.1 and 3.2. This section describes setting
up a new protocol.
On the Home screen (Figure 12), press the navigation button that corresponds with NEW.
Pressing NEW will bring up the Edit screen (Figure 18), where the user can set the three parameters of
current (maximum 2.5 A), voltage (maximum 25 V), and time (maximum 90 min) using the alphanumeric
keypad. The user can toggle between setting a constant voltage (CONST V) or a constant current
(CONST A) using a navigation button. After the settings have been determined, a prompt will appear
to name and save the protocol for subsequent use, if desired. A saved protocol can later be accessed
under LIST > USER-DEFINED protocols. Saved protocols can be deleted when no longer required.
Fig. 18. Edit NEW protocol screen.
Selection buttons for
voltage, current, and
time
Toggle between CONST A and CONST V
Selecting USER-DEFINED protocols accesses the protocols that have been previously saved by users (Figure
17). Use the top and button Selection buttons to scroll to the appropriate choice. Push RUN to go to the
system run screen where a transfer can be started.
To prevent damage to the cassette or instrument, the Trans-Blot Turbo system will terminate a transfer if
30 watts per hour is exceeded over the course of the run. Depending on the initial run conditions when
designing a protocol, the system may display a message warning that the 30 watts/hour limit may be
exceeded during the run.
14 Trans-Blot® Turbo™ Blotting System