BIO RAD Trans-Blot Turbo, Instruction Manual

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3.5 Transfer Using Preprogrammed Protocols
All steps are identical to those in Sections 3.3 and 3.3.a, except for Section 3.3, step 2, which is
described here.
On the Home screen press the Navigation button that corresponds with LIST (Figure 12) to choose
between the Bio-Rad preprogrammed protocols and user-defined protocol lists (Figure 16).
Pressing the Bio-Rad preprogrammed protocols button accesses the protocols described in Table 3.
• STANDARD SD provides typical semi-dry transfer conditions for use with conventional semi-dry
western blotting consumables (see Section 3.6)
• 1.5 mm GEL uses a longer transfer time (10 min) for more efficient transfer when using 1.5 mm thick
gels
• HIGH MW is optimized for more efficient transfer of large proteins (>150 kDa)
•
LOW MW
is optimized for more efficient transfer of small proteins (<30 kDa)
•
MIXED MW
is for efficient transfer of proteins over a broad range of molecular weights (5–150 kDa).
This protocol is also accessed via the Turbo Navigation button
•
Mini-TGX
is an ultrafast protocol that will transfer a single Mini-PROTEAN TGX gel with mixed MW
proteins (5–150 kDa) in 3 min with excellent efficiency.
Table 3. Bio-Rad preprogrammed protocols.
Fig. 16. Protocol options.
Protocol Name MW (kDa) Time (min)
2 Mini Format Gels or
1 Midi Format Gel
(per cassette)
1 Mini Format Gel
(per cassette)
Standard SD Any 30 Up to 1.0 A; 25 V
1.5 mm GEL Any 10
2.5 A; up to 25 V 1.3 A; up to 25 V
High MW >150 10
Low MW <30 5
Mixed MW (Turbo) 5–150 7
1 Mini-TGX 5–150 3 – 2.5 A; up to 25.0 V
Trans-Blot® Turbo™ Blotting System 13