Section 5
Guidelines for Using the Aurum
Plasmid Mini Kit
Please read the following guidelines before starting the plasmid purification.
Bacterial Growth Guidelines:
• The Aurum plasmid mini kit can process cultures grown in a variety of
different broths, such as LB (Luria-Bertani broth), LBG (LB + 2% glycerol),
SB (Super Broth) and 2x YT. For optimum performance, LB or LBG is
recommended for most strains of
E. coli.
TB (Terrific Broth) cultures
generally produce lower plasmid yields of more variable quality and are
therefore not recommended.
• For optimum plasmid quality, plasmid propagation in an
endA-
host, such as
DH5
a
, JM109, or XL1-Blue, is recommended.
• Spectrophotometric determination of culture density is a REQUIREMENT for
optimum performance. Optimized concentrations of purified plasmid will
generally result with most high copy number constructs, bacterial hosts and
3
Fig. 1. Aurum vacuum manifold
