Section 3
Storage Conditions
Solutions and columns should be stored at room temperature. If
precipitation is observed in any solution, warm solution to 37ºC to
redissolve, and allow to return to room temperature before use. Do not
expose any of the solutions to temperatures above 37ºC. If the kit is
used infrequently, storage of the resuspension solution at 4ºC is
recommended to preserve the RNase.
Section 4
Necessary Supplies
Equipment and supplies to be provided by the customer:
•
1.5–2.0 ml capped microcentrifuge tubes
200
•
95–100% ethanol, or reagent-grade (denatured) ethanol, 100 ml
•
Microcentrifuge (12,000 x g)
Additional equipment required for vacuum format:
•
Bio-Rad Aurum vacuum manifold with vacuum regulator and
column adaptor plate (Cat. #732-6470), or other vacuum manifold
with luer fittings (Figure 1).
Note:
Please read Section 7,
Instrument Setup and Use for the
Column Adaptor Plate
in the Aurum vacuum manifold instruction
manual for proper vacuum setup conditions.
•
Vacuum source (capability of -20 to -23" Hg required)
2
