13
Problem
Possible Cause
Possible Solution
More than one band
Presence of multimers –
Try different host or
on analytical gel
typical and variable
growth conditions
depending upon plasmid,
bacterial host, etc.
Nicking due to
Use
endA-
host such as
endonuclease activity
JM109, DH5
a,
or
XL1-Blue
Prolonged exposure to
Add neutralization solution
alkaline conditions
within 5 min after
addition of lysis solution
Plasmid nicked/
Use of
endA+
bacterial
Use
endA-
host such as
degraded
host
JM109, DH5
a,
or
XL1-Blue
Plasmid prep
Plasmid nicked, degraded Check integrity of plasmid
performs poorly in
or denatured
on an analytical gel
enzymatic reactions
Use
endA-
host
Section 8
Ordering Information
Catalog #
Description
732-6470
Aurum Vacuum Manifold,
includes column adaptor plate,
4 replacement luer caps, A and B stages, waste collection
tray, vacuum regulator and gauge, tubing, protocol overview,
and instruction manual
732-6400
Aurum Plasmid Mini Kit,
100 preps, includes 100
plasmid binding columns, 100 capless collection tubes (2.0
ml), reagents, protocol overview, and instruction manual
