BIO RAD Aurum Plasmid Mini Kit, Instruction Manual

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Fig. 6. Aurum plasmid mini protocol overview: spin format
Growth and Isolation
1. Grow 1–5 ml bacterial culture overnight or
16 hr.
2. Measure A
600
(if higher yield required).
3. Transfer an appropriate volume of culture to
a capped 2 ml tube. Centrifuge and
decant supernatant.
4. Add 250 µl resuspension solution; vortex.
5. Add 250 µl lysis solution; invert 6–8x.
6. Add 350 µl neutralization solution; invert 6–8x.
7. Centrifuge 5 min to pellet cell debris.
Purification
8. Transfer cleared lysate (supernatant) to
mini spin/vac column.
9. Centrifuge 1 min to bind plasmid DNA.
Decant flow-through.
10. Add 750 µl wash solution and
centrifuge 1 min. Decant flow-through.
11. Centrifuge additional 1 min to remove
residual wash solution.
Collection of Purified Samples
12. Transfer mini spin/vac column to a clean
1.5–2.0 ml capped tube.
13. Add 50 µl elution solution. Let stand 1 min
and then centrifuge 1 min to elute.
14. Purified DNA is ready to use or can be
stored at 4°C.
Aurum Plasmid Mini Kit: Cat. #732-6400
For more information, call Technical Services
at 1-800-424-6723.
750 µl
wash
solution
50 µl
elution
solution
Aurum ™ Plasmid
Mini Kit:
Spin Format
Protocol Overview
For complete protocol, consult instruction manual.
Transfer cleared lysate
350 µl
neutralization
solution
250 µl
lysis solution
250 µl
resuspension
solution
Invert 6–8x
Vortex
Invert 6–8x
™