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BV200 Plasma Viscometer Mk2/3 Customer User
Manual

Issue 9

Sep 2020 - Rev 1.8

Page 7 of 53

2.8. Uncertainties and Linearity

Viscosity Range

Dynamic Viscosity mPa.s (cP)

0.3 to 7.4

± 0.07 %

7.4 to 10

± 0.09 %

10 to 30

± 0.12 %

The chart above shows the BV200 viscometer test linearity from 0.89 to 9.99 mPa.s. The PV was
measured at 37

℃

but reported at 25°C

Summary of Contents for BV200

Page 1: ...samples so it is designed to handle bio hazardous materials safely All equipment is manufactured produced and supplied by Benson Viscometers Ltd in the UK Only reagents and viscometer cleaning produc...

Page 2: ...ies please call us on 01646 650065 or e mail info bensonviscometers com Benson Viscometers Ltd Unit H North Estate Withybush Road Haverfordwest SA62 4BS Benson Viscometers Ltd 13 Greenwich Business Pa...

Page 3: ...3 non electrical connection points on the side of the BV200 4mm tube push fit connector for the wash bottle 5mm tube push fit connector for the vacuum regulator 6mm tube push fit connector for the was...

Page 4: ...t PSU viscometer is protected by two 3 15 amp fuses located in the IEC inlet socket The viscometer is also protected by an over voltage protection circuit located within the 36 volt PSU The BV200 110...

Page 5: ...or R 300 Vacuum pump Vacuum Regulator Wash and Waste Bottle 4mm 5mm 6mm and 8mm connection hoses Grey PVC base plate with built in liquid level sensors PC A computer PC that consists of Monitor Compu...

Page 6: ...o ensures viscometer result repeatability The second result is stored in the viscometer but marked as void for LIMs and it is not transmitted The 1 6mPa s check control is measured every ten patient s...

Page 7: ...Rev 1 8 Page 7 of 53 2 8 Uncertainties and Linearity Viscosity Range Dynamic Viscosity mPa s cP 0 3 to 7 4 0 07 7 4 to 10 0 09 10 to 30 0 12 The chart above shows the BV200 viscometer test linearity...

Page 8: ...ma Viscometer Mk2 3 Customer User Manual Issue 9 th Sep 2020 Rev 1 8 Page 8 of 53 The chart above shows the BV200 viscometer test linearity from 0 69 to 6 34 mPa s The PV was measured at 37 and report...

Page 9: ...e warning This symbol is essentially a safety symbol and should be used to highlight the fact that there are specific warnings or precautions associated with the device which are not otherwise found o...

Page 10: ...Biological Growth Reagent is toxic if undiluted The anti biological growth reagent used comprises 0 02 Sodium Azide in the LOW HIGH and CHECK reagents so is not toxic at this level but users should be...

Page 11: ...hazardous and handled according to your organisation s health and safety policy When carrying out the scheduled sterilise maintenance process on the waste bottle always wear suitable safety clothing a...

Page 12: ...he viscometer with water could increase the hazard of an electric shock Only trained operators should clean the viscometer Cleaning fluid should never be used near the electrical components of the sys...

Page 13: ...Warnings Type of Warning Precaution When removing and handling a cassette it must be considered to be a Biohazard Always switch the analyser off before removing replacing the cassette Always decontami...

Page 14: ...e precautions to avoid becoming infected by blood products The Anti Biological Growth Reagent is toxic if undiluted The anti biological growth reagent used comprises 0 02 Sodium Azide in the LOW HIGH...

Page 15: ...document Do not locate the viscometer next to an opening window or in direct sunlight as temperature variations can affect result repeatability 3 7 Transport considerations There is no standard packag...

Page 16: ...e hand to support the rear of the unit can then be placed under the instrument Benson Viscometers will then safely relocate the viscometer to the required location 6 and connect and commission the ana...

Page 17: ...e consult the Benson Support Desk Do not use this device in close proximity to sources of strong electromagnetic radiation e g unshielded intentional RF sources as these can interfere with the proper...

Page 18: ...he Benson automated plasma viscometer uses a regulated vacuum to pull fluid samples through a calibrated capillary tube to determine a fluids viscosity The precise time of the fluid passage between tw...

Page 19: ...BV200 Plasma Viscometer Mk2 3 Customer User Manual Issue 9 th Sep 2020 Rev 1 8 Page 19 of 53 4 3 Pump base 4 4 Viscometer...

Page 20: ...ved by post or from a courier Do not store PV samples in a fridge Plasma samples should be stored at room temperature and should never be stored in a fridge If plasma is stored in a fridge cryoprotein...

Page 21: ...play showing the distribution of all tubes that have been tested Empty tubes will have their symbol e g C flashing In the barcode column on the screen there is a number showing the number of dips take...

Page 22: ...top the viscometer after samples have been processed Benson Viscometers recommends a stop flag is inserted into the black carriage after the last sample in that batch to be tested The system will perf...

Page 23: ...azard Do not routinely use Sodium Hypochlorite or bleach products A trained user is required to perform the following daily weekly and monthly maintenance For all other maintenance issues please conta...

Page 24: ...nson Viscometer cleaning agents are used a When using Benson Viscometers cleaning agents i Select Single or Bulk additive as appropriate ii Select Scan Barcode Then scan the barcode on the single use...

Page 25: ...ls Insert 1 full mixed 3 ENZYME tube protein digestive cleaner immediately followed by 1 STERILISER tube For instructions on making up the Enzyme and Steriliser Solutions please see section 6 5 and 6...

Page 26: ...ning agents are used However when not using Benson Viscometers barcoded cleaning agents you will need to seek approval to override this feature from a Senior within the laboratory This can be complete...

Page 27: ...Customer User Manual Issue 9 th Sep 2020 Rev 1 8 Page 27 of 53 a When complete select Click to Finish The viscometer will then process the Enzyme and Steriliser tubes Note This process takes approxim...

Page 28: ...ev 1 8 Page 28 of 53 6 2 Weekly maintenance Click in the Weekly days or due area from the cleaning activities section in the top bar 1 or from the maintenance manu Follow the on screen instructions to...

Page 29: ...emove and clean the drain line filter 3 a When complete select Click to Proceed Follow the on screen instructions to inspect and clean the probe 4 Always remove the probe from the cassette before clea...

Page 30: ...d up and down several times or until any deposit stops being forced through the aspiration hole iv Inspect the holes in the probe to ensure they are clear and clean if necessary using a pin Note do no...

Page 31: ...pillary 5 When looking at the aspiration hole identify how much of the capillary is visible and is occupying the hole Ideally the capillary should only just be visible as in the top picture below If y...

Page 32: ...the waste bottle cap with a cloth 6 soaked in laboratory sterilisation fluid Some staining of the rubber seal is normal and acceptable a When completed select Click to Proceed Follow the on screen in...

Page 33: ...atively enter manually by selecting Manual Entry and use the keyboard to type the barcode from the single use tube or bulk additive iv When complete select Click to Finish a It is recommended that Ben...

Page 34: ...the on screen instructions for Decontamination Clean Stage 1 once 9 a When complete select Press here to continue Follow the on screen instructions for Stage 2 10 a When complete select Press here to...

Page 35: ...th Sep 2020 Rev 1 8 Page 35 of 53 Follow the on screen instructions for Stage 7 11 Note You do not need to empty and refill the wash and waste bottle if already completed as part of the weekly mainte...

Page 36: ...stomer User Manual Issue 9 th Sep 2020 Rev 1 8 Page 36 of 53 6 3 Monthly maintenance Click in the Monthly cleaning from the top bar 1 Follow the on screen instructions to clean the carriages 2 a When...

Page 37: ...reen instructions to rinse the base a To drain the fluid from the base area open the Sump Drain by clicking on the Sump Drain button This process can be repeated if necessary to fully drain all the fl...

Page 38: ...BV200 Plasma Viscometer Mk2 3 Customer User Manual Issue 9 th Sep 2020 Rev 1 8 Page 38 of 53 Follow the on screen instructions and add any additional notes 5...

Page 39: ...oded decontamination additive shown below From the Maintenance Menu on the main screen select do Decontamination Clean 1 The following popup will be displayed with the following text 2 Add the barcode...

Page 40: ...e adaptor and re connect to the Wash Bottle Pour approx 100ml of tap water into the carriage area near the front of the BV200 unit so it drips down into the drainage channels below the carriages to fl...

Page 41: ...BV200 Plasma Viscometer Mk2 3 Customer User Manual Issue 9 th Sep 2020 Rev 1 8 Page 41 of 53 Once complete click the Press here to continue button 5...

Page 42: ...the enzyme is only mixed when it is needed this should not be an issue To Commence Enzyme Cleaning 2 An enzyme clean will commence when a tube displaying an ENZYME label is read by the barcode reader...

Page 43: ...en has a 14 day use by date As the steriliser is only mixed when it is needed this should not be an issue To Commence a Steriliser Clean 2 A steriliser clean will be carried out when a tube displaying...

Page 44: ...on the Drain Valve button whilst observing the waste vacuum gauge 7 The vacuum pressure should only drop by about 0 05 bar and then hold steady 8 If the vacuum level drops greater than 0 05 bar pleas...

Page 45: ...Flush the entire contents of the syringe through the filter ensuring that the waste is 5 going in to a dirty sink If debris remains in the filter repeat steps 3 5 6 Fluid can pushed and sucked through...

Page 46: ...available For 75 x 13 ml tubes transfer only a small amount of plasma 0 4 to 0 5 ml maximum to a 1 5 ml hanging cup or 1 5 ml mini tube using a Pasteur Place the hanging cup or mini tube in an empty p...

Page 47: ...just the regulator so the runtime is 2 00 0 05 Vacuum on waste bottle vac gauge should be a minimum of 0 70 Make sure the cap on the waste bottle is fully tightened and the cap tubes are fully inserte...

Page 48: ...into an Steriliser labelled empty collection tube Add 4 drops of the Steriliser Additive from the 30ml dropper to the tube Replace the cap of the tube and mix well Remove the cap and present the tube...

Page 49: ...witch on power to the Viscometer 2 Open the front cover of the Viscometer 3 Sweep the barcode strips through the red barcode beam from set to end 4 As the barcode reader scans the set barcode the soun...

Page 50: ...ic Shelf life 1 year from fill date the expiry date is shown on the box and on the tube 9 5 Control 2 5 6 dip units per Sarstedt 2 7 tube contains glycerine and Sodium Azide Toxic Shelf life 1 year fr...

Page 51: ...uge If possible mark the 1 position of the needle on the gauge and the adjustment knob For regulators with a light grey regulator knob release the lock on the vacuum regulator 2 valve by gently liftin...

Page 52: ...Untreated myeloma plasma frequently has viscosity values of 3 mPa s while plasma containing macroglobulins can have values 10 mPa s 11 1 Samples Required Plasma from EDTA anticoagulated peripheralven...

Page 53: ...ition however when taken in conjunction with a clinical assessment a result in the range of 1 75 2 00 mPa s can give an indication of an underlying problem Malignant disease 3 Early stage malignancy m...

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