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Doc ID: 76955 Rev. 2.0

BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide

16

Maintaining the BD Rhapsody™ Express 
instrument

Introduction

This section describes how to maintain and decontaminate the 
BD Rhapsody Express instrument.

General 
maintenance 
guidelines

Use 10% (v/v) bleach, 70% (v/v) ethyl alcohol, or 70% (v/v) 
isopropyl alcohol to clean the BD Rhapsody Express instrument.

Cleaning the drip 
tray and tube 
receptacles

1. During cleaning, wear suitable protective clothing, eyewear, 

and gloves.

2. After each use, move the front slider to 

OPEN

:

OPEN

WASTE

BEADS

Summary of Contents for BD Rhapsody Express

Page 1: ...n and Company BD Biosciences 2350 Qume Drive San Jose CA 95131 USA Tel 1 877 232 8995 prompt 2 2 researchapplications bd com BD Rhapsody Express Single Cell Analysis System Installation and Maintenance Guide For the BD Rhapsody Express instrument ...

Page 2: ...r any damages resulting from the application or use of this information BD Biosciences welcomes customer input on corrections and suggestions for improvement Regulatory information For Research Use Only Not for use in diagnostic or therapeutic procedures Patents The BD Rhapsody Targeted mRNA and AbSeq Reagent Kit is covered by one or more of the following U S patents 8835358 9315857 9290808 929080...

Page 3: ...ntenance 15 Maintaining the BD Rhapsody Express instrument 16 Returning the BD Rhapsody Express instrument for service 20 Maintaining the BD Rhapsody P1200M and P5000M pipettes 20 Returning the BD Rhapsody P1200M and P5000M pipettes for service 21 Chapter 4 Troubleshooting 23 BD Rhapsody Express instrument troubleshooting 24 Appendix A Safety 27 General safety and limitations 28 Chemical safety 28...

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Page 5: ...Doc ID 76955 Rev 2 0 1 Introduction ...

Page 6: ... BD Rhapsody Single Cell Analysis system see the BD Rhapsody Express Single Cell Analysis System Instrument User Guide Doc ID 214063 For detailed site requirements and technical specifications see the BD Rhapsody Express Single Cell Analysis System Site Preparation Guide Doc ID 75750 Genomics technical publications are available for download from the BD Genomics Resource Library at bd com genomics...

Page 7: ...ionary information such as warnings and precautions that cannot for a variety of reasons be presented on the device itself Biological hazard All surfaces that come in contact with biological specimens can transmit potentially fatal disease Use universal precautions when cleaning surfaces Wear suitable protective clothing eyewear and gloves Lifting hazard ...

Page 8: ... This topic describes how to get technical support for the BD Rhapsody Express instrument Contacting technical support If technical assistance is required contact researchapplications bd com or 1 877 232 8995 prompt 2 2 or 353 61 307734 You can contact technical support in Europe at help biosciences europe bd com or at these telephone numbers Location Telephone number Location Telephone number Wor...

Page 9: ...e Product name model number and serial number on the rear of the instrument Details of recent system performance Germany 069 22 22 25 60 Sweden 08 50 69 21 54 Greece 00800 12 75 06 Switzerland 044 58 04 373 Italy 02 36 00 36 85 United Kingdom 0207 07 53 226 Location continued Telephone number Location Telephone number ...

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Page 11: ...Doc ID 76955 Rev 2 0 2 Installation ...

Page 12: ...ite Prepare the site for the Express instrument See the BD Rhapsody Express Single Cell Analysis System Site Preparation Guide Doc ID 75750 Installation Contact BD Biosciences technical support at researchapplications bd com Lifting the BD Rhapsody Express instrument Lift the BD Rhapsody Express instrument by firmly grasping the cutout on each side of the instrument ...

Page 13: ... 76955 Rev 2 0 Chapter 2 Installation 13 Installing the drip tray and splash shield 1 Move the front slider to OPEN 2 Review the location of parts OPEN WASTE BEADS Insert drip tray here Drip tray Splash guard ...

Page 14: ... so that its larger compartment faces upwards and is positioned towards the interior of the instrument The compartments of the drip tray correspond to the tube sizes above it 4 Insert the drip tray fully until it stops 5 Move front slider to BEADS 6 Slide the splash shield fully underneath the drip tray until it stops OPEN WASTE BEADS ...

Page 15: ...Doc ID 76955 Rev 2 0 3 Maintenance ...

Page 16: ... how to maintain and decontaminate the BD Rhapsody Express instrument General maintenance guidelines Use 10 v v bleach 70 v v ethyl alcohol or 70 v v isopropyl alcohol to clean the BD Rhapsody Express instrument Cleaning the drip tray and tube receptacles 1 During cleaning wear suitable protective clothing eyewear and gloves 2 After each use move the front slider to OPEN OPEN WASTE BEADS ...

Page 17: ...ollection Container Cat No 650000090 and Bead Retrieval Tube 5 mL LoBind Tube 6 Use a dry lint free wiper to dry the components 7 Orient the white drip tray so that its larger compartment faces upwards and is positioned towards the interior of the instrument and then reinsert the drip tray fully until it stops The compartments of the drip tray correspond to the tube sizes above it 8 Move the front...

Page 18: ...955 Rev 2 0 BD Rhapsody Express Single Cell Analysis System Installation and Maintenance Guide 18 Cleaning the splash shield 1 Move the front slider to BEADS for access to the splash shield tab OPEN WASTE BEADS ...

Page 19: ...yl alcohol 5 Use a dry lint free wiper to dry the splash shield 6 Slide the splash shield fully into the left opening of the instrument until it stops 7 Dispose of the used cleaning materials in biohazard containers Cleaning the cartridge tray 1 Remove the BD Rhapsody Cartridge if present 2 Use a dry lint free wiper to dry the cartridge tray and then use a lint free wiper moistened with 10 bleach ...

Page 20: ...iences 3 Contact BD Biosciences at researchapplications bd com for return authorization 4 Complete the return shipping label 5 Ship the BD Rhapsody Express instrument to BD Biosciences according to shipping instructions on the label Maintaining the BD Rhapsody P1200M and P5000M pipettes Introduction This section describes how to maintain and decontaminate the BD Rhapsody P1200M and P5000M pipettes...

Page 21: ... calibration follow the cleaning protocol See Maintaining the BD Rhapsody P1200M and P5000M pipettes page 20 2 Complete the decontamination label provided by BD Biosciences 3 Obtain the purchase order number required to pay for calibration by the Gilson Service Center In the purchase order for either pipette include one of these part numbers LVL3SBD Non ISO 17025 calibration LVL3SISOBD ISO 17025 c...

Page 22: ... Guide 22 4 Ship the pipettes to the appropriate Gilson Service Center Location Shipping address USA Gilson Service Center 3101 Laura Lane STE 100 Middleton WI 53562 USA Europe Gilson International France SAS Attention OEM Department 19 Avenue des Entrepreneurs CS 55501 95400 VILLIERS LE BEL FRANCE ...

Page 23: ...Doc ID 76955 Rev 2 0 4 Troubleshooting ...

Page 24: ...xpress instrument Difficulty removing the cartridge from the BD Rhapsody Express instrument Possible causes Recommended solutions Cartridge remains clipped at the rear of the tray Push the cartridge on the tray toward the back of the BD Rhapsody Express instrument to compress the metal clip and then lift the cartridge to remove it If the cartridge does not detach from the instrument contact BD Bio...

Page 25: ...f the Retrieval top magnet is not away from the cartridge tray lift and push the Retrieval magnet away from the cartridge tray Push magnet away from tray Magnet away from tray Possible causes Recommended solutions Various Clean the splash shield See Cleaning the splash shield page 18 and reinstall it See Installing the drip tray and splash shield page 13 Ensure you install the splash shield fully ...

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Page 27: ...Doc ID 76955 Rev 2 0 A Safety ...

Page 28: ...m Instrument User Guide Doc ID 214063 Genomics technical publications are available for download from the BD Genomics Resource Library at bd com genomics resources Chemical safety Waste disposal Dispose of waste using proper precautions and in accordance with local regulations Waste disposal Disposal of the instrument Contact BD Biosciences technical support at researchapplications bd com before d...

Page 29: ...Doc ID 76955 Rev 2 0 Glossary ...

Page 30: ...Express instrument Mechanical instrument used for loading the Cell Capture Beads and cells into the BD Rhapsody Cartridge BD Rhapsody Express Single Cell Analysis system The system includes BD Rhapsody Express instrument BD Rhapsody P1200M pipette BD Rhapsody P5000M pipette Bioinformatics software and sequencing analysis ...

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