Beckman Coulter Microfuge 16 Instructions For Use Manual Download Page 54

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Summary of Contents for Microfuge 16

Page 1: ...PN A49436AG February 2014 Instructions For Use Microfuge 16 Centrifuge FX241 5P and FX121 5P Rotors Beckman Coulter Inc 250 S Kraemer Blvd Brea CA 92821 U S A...

Page 2: ...ks Beckman Coulter the stylized logo Microfuge and Beckman Solution 555 are trademarks of Beckman Coulter Inc and are registered in the USPTO All other trademarks service marks products or services ar...

Page 3: ...on which if not avoided may result in minor or moderate injury It may also be used to alert against unsafe practices IMPORTANT IMPORTANT is used for comments that add value to the step or procedure be...

Page 4: ...rve the following Use only the rotors and accessories designed for use in this centrifuge Before starting the centrifuge make sure that the rotor tie down screw is securely fastened Do not exceed the...

Page 5: ...a Beckman Coulter centrifuge that has been modified without Beckman Coulter s approval Handle body fluids with care because they can transmit disease No known test offers complete assurance that such...

Page 6: ...ements China RoHS Caution Label This label indicates that the electronic information product contains certain toxic or hazardous substances The center number is the Environmentally Friendly Use Period...

Page 7: ...afety Notice v RoHS Notice vi China RoHS Caution Label vi China RoHS Environmental Label vi CHAPTER 1 Introduction 1 1 Certification 1 1 Scope of Manual 1 1 Conventions 1 2 CFC Free Centrifugation 1 2...

Page 8: ...3 2 Prerun Safety Checks 3 2 Rotor Preparation 3 2 Starting a Timed or Continuous Run 3 5 RCF Display 3 7 Starting a Short Pulse Run 3 7 Removal and Sample Recovery 3 8 CHAPTER 4 Troubleshooting 4 1 T...

Page 9: ...or Rotor 5 5 Supply List 5 6 Replacement Parts 5 6 Supplies 5 6 APPENDIX A Installation A 1 Installing the Centrifuge A 1 Electrical Requirements A 2 Test Run A 3 Beckman Coulter Inc Microfuge 16 Cent...

Page 10: ...Illustrations Illustrations 2 1 The Microfuge 16 Centrifuge 2 1 2 2 The Control Panel 2 4 3 1 Installing the Rotor and Tightening the Tie Down Screw 3 3 A 1 Dimensions of the Microfuge 16 Centrifuge A...

Page 11: ...bles Tables 2 1 Available Tubes and Adapters for the FX241 5P and FX121 5P Rotors 2 11 3 1 RCF Values at Various Speeds for the FX121 5P Rotor 3 7 4 1 Troubleshooting Chart 4 1 4 2 Error Message Chart...

Page 12: ...xii Tables...

Page 13: ...ntenance CHAPTER 2 Description contains system specifications and a brief physical and functional description of the centrifuge including the operating controls and indicators CHAPTER 3 Operation cont...

Page 14: ...Electronic Equipment WEEE Directive of the European Union The presence of this marking on the product indicates 1 the device was put on the European market after August 13 2005 and 2 the device is not...

Page 15: ...er FX241 5P and FX121 5P rotors designed specifically for use in this centrifuge applications include Nucleic acid plasmids and bacteriophages isolation Routine processing such as sample preparations...

Page 16: ...et time 10 sec to 99 min 59 sec in 1 second increments continuous run or short run pulse Ambient temperaturea a The Microfuge 16 centrifuge can be used in a humidity controlled cold room 4 C 39 F to 4...

Page 17: ...oor The diecast door has a strobe port for speed verification The door is secured by a solid hinge at the back of the centrifuge and by a motorized auto lock closure system at the front This system ke...

Page 18: ...following parameters in succession Speed in rpm speed in rcf g Time in minutes and seconds see page 3 7 for more information on time settings Soft slow acceleration and or deceleration Slow accelerati...

Page 19: ...between rpm and rcf START STOP Key The START STOP key is used to Start a timed run by pressing and releasing START STOP The rotor accelerates to the set speed and continues until the time runs down or...

Page 20: ...me remaining timed run setting The optional slow acceleration and deceleration settings soft or soft stop are displayed if selected Short run is displayed during a short pulse run Speed Speed settings...

Page 21: ...ccessed either by selecting a time setting of 10 seconds 0 10 and pressing the down arrow once or by selecting 99 59 and pressing the up arrow once When the START STOP key is pressed the rotor acceler...

Page 22: ...he FX121 5P rotor can centrifuge up to twelve 1 5 to 2 2 mL reaction vials as well as 200 to 750 L vials using adapters The FX241 5P and FX121 5P rotors develop centrifugal forces that can efficiently...

Page 23: ...inal rotor capacity 24 1 5 2 2 mL 36 52 8 mL Approximate acceleration time to maximum speed fully loaded 15 seconds Approximate deceleration time from maximum speed fully loaded 13 seconds Weight of f...

Page 24: ...o the standard acceleration of gravity g according to the following formula RCF r 2 g where r is the radius in millimeters is the angular velocity in radians per second 2 RPM 60 and g is the standard...

Page 25: ...s Description Dimensions Volume Part Number Adapter Max Speed rpm a polyethylene tube with attached cap 11 45 mm 1 8 mL 340196 pkg 500 none 10 000 polypropylene tube with attached cap natural 11 40 mm...

Page 26: ...ications The maximum run speed listed in the rotor specifications is for operation when all conditions are within the standard specifications If nonprecipitating solutions more dense than 1 2 g mL are...

Page 27: ...up require more than one level of protection WARNING The centrifuge must not be used in the vicinity of flammable liquids or vapors and such materials should not be run in the centrifuge Never bring a...

Page 28: ...luid containment is required use capped tubes All containers carrying physiologicalfluids should be capped andnot overfilled to prevent leakage a Verify that the tubes being used are listed in Table 2...

Page 29: ...crew A46544 is in good condition and the threads are free of foreign matter 3 Center the rotor over the drive shaft and carefully lower it straight down see Figure 3 1 Figure 3 1 Installing the Rotor...

Page 30: ...Pull up on the snap on lock and set the lid in place on the rotor a Release the lock then make sure that the lid is firmly in place b Press down on the lock to ensure engagement NOTE If the rotor is l...

Page 31: ...er 20 seconds 3 Press the MENU key until m and s flash in the display The time setting can be adjusted 4 Timed run press the up and or down arrow keys to select a run time The time setting is saved af...

Page 32: ...and soft stop settings continue to be displayed after 20 seconds 8 Check that the door is shut and securely latched 9 Press START STOP Timed run The time remaining is displayed and begins to count dow...

Page 33: ...centrifuge display apply to the FX241 5P rotor For the FX121 5P rotor maximum RCF values at various speeds are shown in Table 3 1 Starting a Short Pulse Run 1 Check that the door is shut and securely...

Page 34: ...attempt to override the door interlock system while the rotor is spinning Removal and Sample Recovery CAUTION If disassembly reveals evidence of leakage you should assume that some fluid escaped the r...

Page 35: ...such as speed or load type Note any unusual environmental and or operating conditions such as ambient temperature or voltage fluctuations Add any other information that may be helpful Table 4 1 Troubl...

Page 36: ...turn power on I If power cannot be restored see Accessing the Rotor in Case of Power Failure below to retrieve your sample Table 4 1 Troubleshooting Chart Continued Problem Probable Cause Recommended...

Page 37: ...g Never attempt to override the door interlock system while the rotor is spinning 1 Turn the power switch to off O and unplug the power cord from the power source The display is inactive 2 Make sure t...

Page 38: ...ooting Accessing the Rotor in Case of Power Failure 5 Remove the tool return the instrument to its normal position and remove the rotor WARNING Never try to slow or stop the rotor by hand 6 Open the d...

Page 39: ...ock or personal injury Make sure that the power switch is off O and the centrifuge is disconnected from the main power source by removing the Mains power plug from the outlet receptacle and refer such...

Page 40: ...mpatibilities of rotor and accessory materials Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center Cleaning Frequent cleaning will...

Page 41: ...ith water b Rinse thoroughly and dry completely c Lubricate the drive shaft with Spinkote 306812 after cleaning 3 Wash the bowl using a mild detergent such as Solution 555 a Rinse thoroughly and dry c...

Page 42: ...genic solutions follow appropriate decontamination procedures as outlined by your laboratory safety officer Refer to Chemical Resistances to be sure the decontamination method will not damage any part...

Page 43: ...ifuge for an extended period return it to the original shipping container to protect it from dust and dirt Temperature and humidity conditions for storage should meet the environmental requirements de...

Page 44: ...ice Supply List NOTE Publications referenced in this manual can be obtained by calling 1 800 742 2345 in the United States or by contacting your local Beckman Coulter office or visit www beckmancoulte...

Page 45: ...age 2 Select a location away from heat producing laboratory equipment and direct sunlight with sufficient ventilation to allow for heat dissipation 3 Position the centrifuge on a level surface such as...

Page 46: ...with the voltage listed on the name rating plate affixed to the centrifuge Then plug in both ends of the centrifuge power cord Never use a three to two wire plug adapter Never use a two wire extensio...

Page 47: ...rce in case of a malfunction Test Run We recommend that you make a test run to ensure that the centrifuge is in proper operating condition following shipment See CHAPTER 3 for instructions on operatin...

Page 48: ...PN A49436AG A 4 Installation Test Run...

Page 49: ...oulter will repair or at its election replace such component or accessory What constitutes either reasonable service and a reasonable period of time shall be determined solely by Beckman Coulter Repla...

Page 50: ...PN A49436AG Warranty 2 Beckman Coulter Inc Microfuge 16 Centrifuge Warranty...

Page 51: ...only and Beckman Coulter shall not be liable for damage to accessories or ancillary supplies including but not limited to i tubes ii tube caps iii tube adapters or iv tube contents 2 This warranty is...

Page 52: ...PN A49436AG Warranty 4 Beckman Coulter Inc Benchtop Rotor Warranty...

Page 53: ...biol gico Risque biologiqu Pericolo biologico Material infeccioso potencial On power Ein Netzverbindung Encendido Marche mise sous tension Acceso sotto tensione Fora o poder Off power Aus Netzverbindu...

Page 54: ...r Centrifugation Products PN IN 175 Available in hard copy or electronic pdf by request Available at www beckmancoulter com Use and Care of Centrifuge Tubes and Bottles PN IN 192 Available in hard cop...

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