Beckman Coulter Avanti J-26 XP Series Instructions For Use Manual Download Page 50

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J326XP-IM-5AB

3-10

Operation

Entering Run Parameters

Install the rotor.

Set up sample and buffer reservoirs and tubing lines as described in the rotor manual.

Route the tubing lines through the elutriator port holes at the left side of the door seal.

Close the centrifuge door.

Turn the

ROTOR

knob to the

position.

•

5.0 appears in the

ROTOR ID

display.

Enter the remaining run parameters. Refer to the JE-5.0 rotor manual for rotor setup and
operating instructions.

•

The run is ready to begin.

Press

START

•

The rotor accelerates to set speed.

When the rotor reaches set speed, turn on the strobe controls by pressing the strobe power
switch.

•

The power switch indicator lights.

Strobe Power Switch

ROTOR

JSP

JLA

JCF

ELUTRIATOR

DELAY

Summary of Contents for Avanti J-26 XP Series

Page 1: ...J326XP IM 5AB January 2012 Instruction For Use Avanti J 26XP High Performance Centrifuge Beckman Coulter Inc 250 S Kraemer Blvd Brea CA 92821 ...

Page 2: ...n Coulter Inc All rights reserved No part of this document may be reproduced or transmitted in any form or by any means electronic mechanical photocopying recording or otherwise without prior written permission from Beckman Coulter Inc Find us on the World Wide Web at www beckmancoulter com ...

Page 3: ...ion that should be followed during installation use or servicing of this equipment Safety During Installation and or Maintenance This instrument is designed to be installed by a Beckman Coulter Field Service representative Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty coveringtheinstrument Also iftheinstrument needstobe moved a Beckman CoulterField...

Page 4: ...ohol in this instrument nor handle or store them near the centrifuge Mechanical Safety For safe operation of the equipment observe the following Use only the Beckman Coulter rotors and accessories designed for use in this instrument Do not exceed the maximum rated speed of the rotor in use NEVER attempt to slow or stop a rotor by hand Do not lift or move the centrifuge while a rotor is spinning NE...

Page 5: ...taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection Dispose of all waste solutions according to appropriate environmental health and safety guidelines It is your responsibility to decontaminate...

Page 6: ...J326XP IM 5AB vi Safety Notice Chemical and Biological Safety ...

Page 7: ...ii Conventions xiv CFC Free Centrifugation xiv Radio Interference xiv Recycling Label xv CHAPTER 1 Specifications 1 1 Control Features 1 1 Operational Features 1 2 Physical Data 1 2 Available Rotors 1 3 Preinstallation Requirements 1 7 Electrical Requirements 1 8 Space and Location Requirements 1 11 Securing the Centrifuge to the Floor 1 11 Using J2 Series Rotors in the Avanti J 1 13 Checking for ...

Page 8: ...Entering Run Parameters 3 4 Selecting a Rotor 3 4 Setting Run Speed 3 4 Setting Run Time 3 5 Setting Run Temperature 3 7 Setting Acceleration and Deceleration Rates 3 7 Setting Up an Elutriation Run 3 9 Starting a Run 3 11 Changing Parameters During a Run 3 12 Changing Time Settings 3 12 Stopping a Run 3 12 Unloading the Centrifuge 3 13 CHAPTER 4 Introduction 4 1 User Messages 4 1 Accessing the Ro...

Page 9: ...ix Contents Storage and Transport 5 5 Storage 5 5 Returning a Centrifuge 5 5 Supply List 5 6 Replacement Parts and Supplies 5 6 APPENDIX A Introduction A 1 Avanti J Series Centrifuge Warranty Warranty 1 ...

Page 10: ...ions 1 10 1 4 Rear View and Dimensions 1 12 1 5 Checking the Rotor for Drive Pins 1 13 2 1 The Power Switch 2 4 2 2 The Control Panel 2 4 3 1 Acceleration and Deceleration Settings Graph 3 8 3 2 Elutriation Controls 3 9 4 1 Emergency Door Release Latch Access 4 5 4 2 Manual Door Release 4 6 4 3 Venting the Chamber Vacuum 4 8 4 4 Front Panel Retaining Strip 4 9 ...

Page 11: ... Tables Tables 1 1 Nominal Supply Voltage Ratings for the Avanti J 26XP 1 8 1 2 Required Wire Connections 1 10 3 1 Acceleration Settings 3 7 3 2 Deceleration Settings 3 8 4 1 Diagnostic Message Chart 4 2 ...

Page 12: ...xii Tables ...

Page 13: ...ons and Preinstallation Requirements contains system specifications and instructions for preparing your site for centrifuge installation CHAPTER 2 Description contains an overall description of the centrifuge including a description of system controls and indicators CHAPTER 3 Operation summarizes procedures for operating the centrifuge CHAPTER 4 Troubleshooting lists system diagnostic messages tog...

Page 14: ... with the limits for a Class A digital device pursuant to Part 15 of FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with this instruction manual may cause interference to radio ...

Page 15: ... device is not to be disposed via the municipal waste collection system of any member state of the European Union It is very important that customers understand and follow all laws regarding the proper decontamination and safe disposal of electrical equipment For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back prog...

Page 16: ...J326XP IM 5AB xvi Introduction Recycling Label ...

Page 17: ...increments Elutriation speed setting range 0 to 5 000 rpm in 10 rpm or 10 g increments Speed display from 0 to 10 000 rpm display indicates actual rotor speed 10 rpm from 10 000 to 26 000 rpm indicates actual rotor speed 0 1 or equivalent RCF Time Setting range to 180 minutes or continuous hold Time display Indicates time remaining timed run or time elapsed hold run Temperature Setting range 10 to...

Page 18: ... may not be able to achieve some higher temperatures Specifications Description Specifications Description Door 6 1 cm 2 4 in thick structural foam with steel plate Rotor Chamber Diameter 51 3 cm 20 in Friction Reduction System FRS 190 mm 7 5 in Hg Specification Description Width 71 cm 28 in Depth 86 cm 34 in including air diverter extending from back panel 102 cm 40 25 in Height with door closed ...

Page 19: ...ditions 2 0 kW 6900 BTu h Humidity restrictions 95 noncondensing Noise Level 0 91 m 3 ft in front of instrument at 26 000 rpm 57 dBa Installation overvoltage Category II Pollution Degree 2b a Unbalanced three phase Split for single phase operation internally b Normally only nonconductive pollution occurs occasionally however a temporary conductivity caused by condensation must be expected Specific...

Page 20: ... 51 500 43 900 32 15 mL J TB 022 JA 20 Fixed Angle 34 8 place rmax 108 mm 20 20 000 48 400 8 50 mL J TB 003 JA 18 1 Fixed Angle 24 place 45 adapter rmax 116 mm 25 adapter rmax 112 mm 18 1 18 000 17 000 42 100 36 300 24 1 8 mL 24 1 8 mL J TB 037 JA 18 Fixed Angle 23 10 place rmax 132 mm 18 18 000 47 900 10 100 mL J TB 035 JA 17 Fixed Angle 25 14 place rmax 132 mm 17 17 000 39 800 14 50 mL J TB 017 ...

Page 21: ...4 mm 12 12 000 23 200 12 50 mL J TB 051 JA 10 Fixed Angle 25 6 place rmax 158 mm 10 10 000 17 700 6 500 mL J TB 006 JLA 10 500 Fixed Angle 20 6 place rmax 166 mm 10 500 10 000 18 600 6 500 mL J TB 048 F10BCI 6x500y Fixed Angle 23 6 place rmax 158 mm F500 previ ously 10 1 10 000 17 696 6 500 mL JLA 9 1000 Fixed Angle 20 4 place rmax 185 mm 9 1000 9 000 16 800 4 1000 mL J TB 073 JLA 8 1000 Fixed Ang...

Page 22: ... 200 6 15 mL J TB 058 JS 7 5 S Swinging Bucket 4 place rmax 165 mm 7 5 7 500 10 400 4 50 mL J TB 007 JS 5 3 Swinging Bucket 4 place rmax 194 8 mm 5 3 5 300 6 130 24 microplates 8 deep well plates 4 square well plates J TB 089 JS 4 3 Swinging Bucket 4 place rmax 204 mm 4 3 4 300 4 220 4 750 mL J TB 050 JS 4 0 Swinging Bucket 4 place rmax 226 mm 4 0 4 000 4 050 4 1 liter 4 blood bags 12 microplates ...

Page 23: ... rmax large chamber 168 mm standard chamber 125 mm Sanderson chamber 126 mm 5 0 5 000 4 700 3 500 4 230 40 mL 4 0 mL 5 5 mL JE5 IM a Maximum speeds are based on a solution density of 1 2 g mL with the following exceptions the JA 18 1 rotor is rated for a density of 1 4 g mL the JCF Z rotor is rated for a density of 1 45 g mL and the JE 5 0 rotor is rated for a density of 3 g mL b Temperature perfo...

Page 24: ...sure safety connect the instrument to a remote emergency switch preferably outside the room where the centrifuge is housed or adjacent to the exit from that room to disconnect the centrifuge from the main power source in case of a malfunction WARNING To reduce the risk of electrical shock this equipment uses a three wire or five wire electrical cord and plug to connect the centrifuge to earth grou...

Page 25: ... three phase centrifuges including earth ground and three power leads with minimum 16 ampere circuit breakers and common neutral connection Table 1 2 contains wiring information Figure 1 1 Single Phase Electrical Requirements Figure 1 2 Three Phase Y Electrical Requirements 1 30 ampere Circuit Breaker 2 Wall Outlet Hubell 9930 Bryant 96 30 FR or Equivalent NEMA 6 30 R 3 Earth Ground 4 Measured Lin...

Page 26: ... a motor start delay type The centrifuge can tolerate a drop of 15 volts during the start period at low line voltage see Figure 1 3 for the acceptable voltage ranges The mains power for the centrifuge must be of sufficient wiregauge to provide thiscondition The wire gauge required at aparticularsite must be determined by a facilities engineer at that site Figure 1 3 Correct and Incorrect Three Pha...

Page 27: ...ntrifuge be sure to move the cord out of the way before pushing the centrifuge towards the wall WARNING Do not place the centrifuge near areas containing flammable reagents or combustible fluids Vapors from these materials could enter the instrument s air system and be ignited Securing the Centrifuge to the Floor Avanti J series centrifuges are certified to meet the requirements of the European CE...

Page 28: ...s of an adhesive backed mounting plate is CSA certified for use on epoxy aggregate floors only Non Invasive Centrifuge Restraint System for Vinyl Floors For laboratories with vinyl tile floors or vinyl seamless floors a non invasive installation kit PN A84005 is available The kit which consists of an adhesive backed mounting plate is CSA certified for use on vinyl floors only 1 Air Exit 2 Power Ca...

Page 29: ...ve pins in the rotor drive hole see Figure 1 5 These drive pins engage with the centrifuge spindle hub to ensure that the rotor does notslip duringacceleration SomeBeckman Coulterrotors includingtheJA 10 the JS 7 5 theJA 14 and the JS 13 have been manufactured without drive pins because pins were not needed when these rotors were used in J2 series centrifuges Figure 1 5 Checking the Rotor for Driv...

Page 30: ...rts are contained in the Zonal Bracket Kit part number 366431 The Zonal Bracket Kit includes a bracket mounting hardware and assembly instructions This kit is for use with Cole Parmer tubing size 16 6 4 mm 1 4 in O D the tubing size most commonly used with the JCF Z rotor Two additional kits are available one which contains adapters for size 14 tubing 4 8 mm 3 16 in O D and one which contains adap...

Page 31: ...urs replace the bearings See the JCF Z rotor instruction manual publication JCFZ IM for bearing replacement instructions Be sure to follow instructions in the next paragraph below for wearing in the bearings On the first few uses of a new JCF Z rotor or when the bearings have been replaced in an older JCF Z rotor lubrication has not yet been thoroughly distributed around the bearings in the bearin...

Page 32: ...J326XP IM 5AB 1 16 Specifications and Preinstallation Requirements Using J2 Series Rotors in the Avanti J ...

Page 33: ...in precipitates large particles and cell debris Preparation of subcellular organelles such as mitochondria nuclei chloroplasts and crude microsomes Separation of blood cells and cellular components Pelleting of prokaryotic and eukaryotic cells Gradient separation for example Ficoll Hypaque and Percoll Nucleic acid precipitation Virus isolation Bacteriophase isolation The Avanti J 26XP centrifuge i...

Page 34: ...system during operation causing automatic shutdown if rotor loads are severely out of balance Housing and Door The instrument control housing is made of aluminum and molded structural foam The door and structural foam cover panels are finished with polyurethane enamel The control panel is covered by a protective overlay made of coated polycarbonate The door is opened by stepping on a foot pedal wh...

Page 35: ...he end of a run the system continues controlling the temperature to prevent freezing or overheating more than 4 C over set temperature of the sample Overtemp System An overtemp over temperature system provides sample protection and safety for the user During a run if the system temperature rises 4 C above the set temperature the TEMP diagnostic will light The run will continue unless stopped by th...

Page 36: ...on The display shows both actual centrifuge values and user set parameters The SET ACTUAL key is used to toggle between the two views During a run actual values are displayed If you turn a knob other than the TIME knob during operation the display will change to show the set values If no parameters are entered or changed for 5 seconds the display will revert to showing actual values During a timed...

Page 37: ...h Beckman Coulter rotor that can be run in the centrifuge appears in succession in the ROTOR ID display and the LED by each rotor type abbreviation JCF continuous flow rotors JSP special type rotors JS swinging bucket rotors JA fixed angle rotors JLA lightweight fixed angle rotors JV vertical tube rotors JE elutriator rotors lights when appropriate SPEED Selection The RPM RCF key is pressed to sel...

Page 38: ...pressed If you switch from HOLD mode to HR MIN mode during a run the system remembers the previous time setting Turning the TIME knob increments or decrements the set time from that point TEMPERATURE Knob Used to select the rotor temperature from 10 to 40 C The minimum and maximum allowable set temperatures depend on the set speed and the rotor in use If a temperature is entered that cannot be ach...

Page 39: ...ion begins STOP Pressed to end a run the centrifuge decelerates to a complete stop according to the deceleration setting The red STOP light blinks during deceleration Deceleration can be terminated and the centrifuge restarted by pressing START CLEAR Pressed to stop the flashing of a diagnostic indicator light See CHAPTER 4 Troubleshooting for information on diagnostic messages SET ACTUAL Pressed ...

Page 40: ...J326XP IM 5AB 2 8 Description Controls and Indicators ...

Page 41: ...ertain systemic fungi further emphasize the need for aerosol protection Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper precautions for aerosol containment Do not run toxic pathogenic or radioactive materials in this centrifuge without taking appropriate safety precautions Biosafe c...

Page 42: ...required run speed rpm appears in the SPEED display Or press RPM RCF to select RCF mode and turn the SPEED knob until the required rcf appears in the SPEED display 5 Turn the TIME knob until the required run length appears in the TIME display Or press TIME HOLD for continuous operation 6 Turn the TEMP knob until the required temperature appears in the TEMP C display 7 Press ACCEL repeatedly to sel...

Page 43: ...otor onto the drive hub The drive shaft can be bent if the rotor is forced sideways or dropped onto the hub Install the rotor by centering it over the hub and carefully lowering it straight down 4 Securely attach the rotor lid knob or tie down knob in rotors without lids to the drive shaft by turning it to the right clockwise NOTE If the knob turns loosely and you do not feel threads engaging the ...

Page 44: ...ed Enter a run speed up to 26 000 rpm or up to the maximum speed of the rotor in use Or enter a relative centrifugal field RCF value up to the maximum achievable RCF of the rotor in use Setting RPM 1 Check to see if the red RPM light is lit on the display RPM is the default speed mode a If it is lit go to step 3 b If the centrifuge is already in the RPM mode skip step 2 and go on to step 3 2 Press...

Page 45: ...CF 1 Check to see if the red RCF light is lit on the display a If it is lit go to step 3 2 Press the RPM RCF key to select the RCF mode On the display the red RCF g LED lights 3 Turn the SPEED knob until the required RCF value appears in the SPEED display The centrifuge operates at a speed calculated to produce the set RCF value a Press RPM RCF to display the RPM value Setting Run Time Pressing th...

Page 46: ...s to accelerate and the display begins showing the remaining run time The run will end when the time counts down to 0 or when STOP is pressed Continuous HOLD Run 1 Press the TIME HOLD key to select the HOLD mode The red HOLD indicator light comes on in the TIME display 2 When all run parameters are entered press START The rotor begins to accelerate and the display begins showing the elapsed time T...

Page 47: ...ation NOTE The system controls the chamber temperature to within 2 C of the set temperature To control temperature within 1 C of set perform the temperature calibration procedure provided in the Appendix Setting Acceleration and Deceleration Rates Table 3 1 and Table 3 2 describe the acceleration and deceleration settings Figure 3 1 provides a graphic representation of these settings During transi...

Page 48: ...s used during the last few rpm until 0 is reached to minimizedisturbanceofthe sample Reduced torque is used from set speed to 500 rpm causing deceleration to 500 rpm to take twice as long as maximum deceleration The exact rate depends on the mass of the rotor in use Larger rotors will take longer Deceleration time from 500 to 0 rpm is approximately 2 minutes larger rotors take longer No brake is u...

Page 49: ... deceleration settings The selected setting lights Setting Up an Elutriation Run Centrifuges equipped for elutriation have elutriation controls as shown in Figure 3 2 along with a view port in the door and an elutriation power box mounted on the back of the control panel See the JE 5 0 elutriator rotor manual JE5 IM for complete rotor setup and operating instructions Figure 3 2 Elutriation Control...

Page 50: ...ge door 3 Turn the ROTOR knob to the JE position 5 0 appears in the ROTOR ID display 4 Enter the remaining run parameters Refer to the JE 5 0 rotor manual for rotor setup and operating instructions The run is ready to begin 5 Press START The rotor accelerates to set speed 6 When the rotor reaches set speed turn on the strobe controls by pressing the strobe power switch The power switch indicator l...

Page 51: ...use a diagnostic shut down during the next run Starting a Run 1 Check that all parameters are correct and the door is firmly closed 2 Press START The display will start showing actual centrifuge values within 5 seconds The LED on the START key flashes during acceleration When set speed is reached the light stays on continuously until the centrifuge begins decelerating As the run begins the system ...

Page 52: ... display the run will end 2 To set a completely new run time while a timed run is in progress turn the TIME knob to the new time setting and press START The system will ignore any elapsed time 3 To change from HR MIN mode to HOLD mode press the TIME HOLD key The display will show the total accumulated run time and will begin counting the time elapsed from that point 4 To change from HOLD mode to H...

Page 53: ...in several locations around the door opening and depress the foot pedal again To minimize icing remove moisture from inside the chamber from the chamber gasket and from the inner door surface before each run by wiping these surfaces with a clean cloth Also keep the door closed as much as possible Unloading the Centrifuge At the end of a run unload the rotor following the instructions in the applic...

Page 54: ...J326XP IM 5AB 3 14 Operation Unloading the Centrifuge ...

Page 55: ...he recommended actions Two kinds of diagnostic conditions can occur Cautionary diagnostic messages alert you to a condition that may need attention when the run is complete Cautionary diagnosticmessages provide information only they do not shut down the run in progress Press CLEAR to stop the light from flashing If the condition still exists the diagnostic message will begin flashing again even th...

Page 56: ...ge Chart Diagnostic Message Problem Result Recommendation FRS friction reduction system FRS integrity is not established within 10 seconds after the pump is turned on Run shuts down with maximum brake Check and clean door sealing area and door gasket Wipe excess moisture from chamber Make sure that elutriator port stoppers are in place at left side of door seal Required FRS level not reached withi...

Page 57: ...ield Service a DOOR Door is not latched properly or interlock is out of position DOOR LED lights after START is pressed and system shuts down Open the door and close it firmly press CLEAR to stop LED from flashing Restart the run Door stays locked after rotor stops spinning Sample cannot be retrieved Restart the centrifuge and perform a brief run If door will still not open after deceleration call...

Page 58: ...otor Follow the directions under Accessing the Rotor in Case of Power Failure below REFR refrigeration system Refrigeration system malfunctioning Run shuts down with maximum brake Call Beckman Coulter Field Service TEMP The refrigeration system is not cooling and the chamber temperature has exceeded 50 C Run shuts down with maximum brake Call Beckman Coulter Field Service During low temperature ru...

Page 59: ... noise coming from the chamber Proceed only if he instrument is quiet Never attempt to override the door interlock system while the rotor is spinning If the facility power fails only momentarily the centrifuge will resume operation when the power is restored and the rotor will return to the set speed In the event of an extended power failure it may be necessary to override the door locking mechani...

Page 60: ...nd latch disengages the front panel will fall forward about an inch from the top The bottom of the front panel is held in place by three grooved brackets attached to the front panel that seat over a metal retaining strip on the centrifuge bottom panel 4 Lift the front panel up and off the centrifuge set it aside You will see an inner front panel that extends about halfway down from the centrifuge ...

Page 61: ...r vacuum grasp the red rubber hose and pull it up until it comes off of the pump fitting see Figure 4 3 a Use a back and forth motion as you pull this takes quite a bit of force The vacuum will be completely vented several seconds after the hose is detached NOTE Several tubing lines are tied to the red hose As you grasp the hose place your hand over these lines Make sure that they stay connected a...

Page 62: ...p with the instrument label facing out insert the three grooved brackets on the bottom inside of the door over the front panel retaining strip see Figure 4 4 a Push the top of the front panel into place 1 Step 1 2 Step 2 3 Step 3 4 Vacuum Pump 5 Grasp red hose and pull up Do not disconnect the tubing lines tied to the red hose 6 Red hose removed from fitting 7 Replace red hose 4 5 6 7 ...

Page 63: ...sidentification has occurred if a rotor name other than JCF Z appears in the ROTOR display during the run when rotor identification is complete If the rotor is misidentified stop the run and follow one of the steps below NOTE Misidentification can be prevented by returning your JCF Z rotor to the Beckman Coulter factory to have magnets added to the rotor body The magnets ensure correct identificat...

Page 64: ... not been replaced recently follow the same procedure as for a new JCF Z rotor perform three runs from 0 to 5000 rpm and back to 0 rpm to ensure that the bearings are properly lubricated If misidentification recurs after the third run when the bearings are lubricated this indicates that the bearings are worn and need to be replaced a Replace the bearings following the instructions in the JCF Z man...

Page 65: ...enance procedure or servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury Make sure that the power switch is off O and the centrifuge is disconnected from the main power source and refer such servicing to qualified service personnel WARNING Do not use alcohol or other flammable substances in or near operating ce...

Page 66: ...uipment Cleaning Clean the centrifuge frequently Always clean up spills when they occur to prevent corrosives or contaminants from drying on component surfaces 1 To prevent accumulations of sample dust and or glass particles from broken sample tubes keep the chamber clean and dry by frequent wiping with a cloth or paper towel a For thorough cleaning wash the chamber using a mild detergent such as ...

Page 67: ...efer to Appendix A in Rotors and Tubes or publication IN 175 to be sure the decontamination method will not damage any part of the instrument Sterilization and Disinfection The centrifuge is finished with acrylic baking enamel on most surfaces see CHAPTER 1 Specifications Ethanol 70 may be used on this surface See Appendix A in Rotors and Tubes or publication IN 175 for chemical resistances of cen...

Page 68: ...e filter straight up until the bottom edge is above the centrifuge bottom retaining strip a Pull the filter out bottom edge first and discard it 3 Install a new filter 885218 a The filter has a directional arrow on one of its edges install the filter with this arrow pointing toward the centrifuge b Holding the filter by the side edges insert the top half behind the frame edge and lift up until the...

Page 69: ...rt or long term storage Temperature and humidity conditions for storage should meet the environmental requirements described under Specifications in CHAPTER 1 Returning a Centrifuge Before returning a centrifuge or accessory for any reason prior permission a Returned Goods Authorization form must be obtained from Beckman Coulter Inc Contact your local Beckman Coulter office to obtain the RGA form ...

Page 70: ...g 6 4 mm 1 4 in O D Zonal Bracket qty 1 Thumbscrew stainless steel M4 threads x 19 mm qty 2 Thumbscrew stainless steel M4 threads x 12 mm qty 2 Cable Clamp nylon 6 4 mm 1 4 in I D qty 10 366431 366430 893412 893411 000499 Zonal Tubing Adapter for Cole Parmer size 14 tubing 4 8 mm 3 16 in O D Note kit 366431 is also required Tubing Adapter stainless steel for size 14 tubing qty 2 Cable Clamp nylon ...

Page 71: ...se as possible to the required temperature 1 For runs at other than ambient temperature precool or prewarm the rotor and test samples to the required temperature The length of precooling prewarming time depends on how different the rotor starting temperature is from the required run temperature 2 Install the rotor with adapters if applicable and tubes or bottles filled with sample buffer or water ...

Page 72: ...sured and set temperatures are different note how many degrees different they are and adjust the set temperature up or down that number of degrees For example If the required sample temperature is And the measured buffer water temperature is Set the temperature to 4 C 6 2 5 4 6 ...

Page 73: ...nd will be returned to Buyer with the transportation charges collect unless the product is found to be defective in which case Beckman Coulter will pay all transportation charges Beckman Coulter makes no warranty concerning products or accessories not manufactured by it In the event of failure of any such product or accessory Beckman Coulter will give reasonable assistance to the Buyer in obtainin...

Page 74: ...J326XP IM 5AB Warranty 2 Beckman Coulter Inc Avanti J Series Centrifuge Warranty ...

Page 75: ...lter les documents joints Atención consulte los documentos adjuntos Attenzione consultare le informazioni allegate On power Ein Netzverbindung Marche mise sous tension Encendido Acceso sotto tensione Off power Aus Netzverbindung Arrêt mise hors tension Apagado Spento fuori tensione Protective earth ground Schutzleiteranschluß Liaison à la terre Puesta a tierra de protección Collegamento di protezi...

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