PN B07493AD
January 2018
Instructions For Use
Allegra X-14 Series
CentrifugesRx Only in the U.S.A.
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
Page 1: ...PN B07493AD January 2018 Instructions For Use Allegra X 14 Series Centrifuges Rx Only in the U S A Beckman Coulter Inc 250 S Kraemer Blvd Brea CA 92821 U S A...
Page 2: ...trademarks service marks products or services are trademarks or registered trademarks of their respective holders Contact Us If you have any questions contact our Customer Support Center Worldwide fi...
Page 3: ...beckmancoulter com and download the latest version of the manual or system help for your instrument Issue AD 01 18 Changes were made to ECREP address Note Changes that are part of the most recent revi...
Page 4: ...PN B07493AD iv Revision History...
Page 5: ...injury It may also be used to alert against unsafe practices IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed Following the advice in the Important adds...
Page 6: ...ed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials such as chloroform or ethyl alcohol in this centrifuge nor handle or store them within th...
Page 7: ...ndcertainsystemicfungi furtheremphasize the need for aerosol protection Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills...
Page 8: ...PN B07493AD viii Safety Notice Chemical and Biological Safety...
Page 9: ...tors 1 3 Power Switch 1 3 Control Panel 1 4 Digital Displays 1 4 Run Parameter Keys 1 5 Name Rating Plate 1 6 Specifications 1 7 Available Rotors 1 9 CHAPTER 2 Operation 2 1 Introduction 2 1 Summary o...
Page 10: ...4 1 Instrument Care 4 1 Maintenance 4 1 Cleaning 4 2 Tube Breakage 4 2 Decontamination 4 3 Sterilization and Disinfection 4 3 Circuit Breaker and Fuses 4 3 Storage and Transport 4 4 Storage 4 4 Suppl...
Page 11: ...xi Illustrations Illustrations 1 1 The Centrifuge 1 2 1 2 Control Panel 1 4 3 1 Door Latch Override 3 6 A 1 Centrifuge Dimensions A 2...
Page 12: ...xii Tables Tables 2 1 Acceleration and Deceleration Rates in Minutes Seconds to and from Maximum Speed 2 6 3 1 Diagnostic Message Chart 3 2 3 2 Troubleshooting Chart 3 5...
Page 13: ...e regulatory agencies Declarations of conformity and certificates of compliance are available at www beckmancoulter com Scope of Manual This manual is designed to familiarize you with the Beckman Coul...
Page 14: ...rotor manual is the sole responsibility of the user Conventions Certain symbols are used in the product labeling to call out safety related and other important information These international symbols...
Page 15: ...gned for use in this centrifuge the centrifuge applications include Routine processing such as sample preparations pelleting extractions purifications concentrations phase separations receptor binding...
Page 16: ...stem prevents the installed rotor from running above its maximum rated speed During acceleration the microprocessor checks the magnetic rotor identification Speed is limited to the maximum safe speed...
Page 17: ...sor calculates the required chamber temperature to maintain the selected rotor temperature Run temperature can be set between 2 and 40 C NOTE To avoid chamber icing refrigeration is off when the door...
Page 18: ...gal field a term describing the ratio of the centrifugal acceleration at a specified radius and speed to the standard acceleration of gravity RCF g TIME In a timed run indicates the remaining run time...
Page 19: ...field If the display shows speed in RCF the key is pressed once to enter speed in RCF or twice to enter speed in RPM Pressed to enter run time using the keypad When TIME is pressed twice HOLD mode is...
Page 20: ...ncludes a plus minus key and a CE clear entry key The CE clear entry key clears any parameter on the display Pressing CE will also clear some diagnostic messages If a diagnostic message does not clear...
Page 21: ...time Temperature Set Temperature Allegra X 14R is 2 to 40 C in 1 increments Allegra X 14 is factory set at 20 C Temperature control after equilibration Allegra X 14R is 2 C of set temperaturea Allegr...
Page 22: ...egree 2b a During transient conditions such as acceleration and deceleration rotor temperature may be outside this range To reach temperatures above ambient the centrifuge is dependent on the friction...
Page 23: ...283 4 750 mL 4 96 mL GX TB 003 SX4750A Swinging Bucket ARIES Tube and bottle buckets rmax 207 8 mm Multiwell plate carriers rmax 183 2 mm SX4750A SX4750a 4 300 4 000 4 303 3 283 4 750 mL 4 96 mL GX TB...
Page 24: ...PN B07493AD 1 10 Description Available Rotors...
Page 25: ...event spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Do not run toxic pathogenic or radioactive materials in this centrifuge without t...
Page 26: ...eration rate number 1 SLOW through 10 MAX 9 Press DECEL then use the keypad or arrow keys to enter the selected deceleration rate number 0 OFF through 10 MAX 10 Check that all parameters are correct a...
Page 27: ...the Rotor The power must be turned on before you can unlock and open the chamber door To end a run for any reason do not turn the power switch off press STOP instead 1 Turn the power switch on The co...
Page 28: ...AVE or another function key is pressed 2 To change an entry before you ve pressed ENTER SAVE or another parameter key enter a different value a Press CE to abort changes and start over b To change an...
Page 29: ...s and 59 minutes or for a hold continuous run The time display begins counting down when the rotor starts to spin For a timed run the run automatically terminates when the set time reaches zero and de...
Page 30: ...t the gradient and sample to gradient interface Select rates depending on the type of run you are performing for pelleting runs where sample mixing is not a concern maximum acceleration and decelerati...
Page 31: ...ART key will not activate If this happens press ENTER SAVE and START again to begin the run The run will end when the time display counts down to zero 6 5 4 3 2 45 3 07 3 36 4 18 2 49 3 15 3 42 4 26 2...
Page 32: ...n runs as follows When the PULSE key is pressed the rotor accelerates at maximum rate to the set speed and continues to spin as long as the PULSE key is pressed The current run time acceleration and d...
Page 33: ...un NOTE If the PROGRAM key is pressed while the program number is flashing program mode is exited without saving entered parameters into program memory Recalling a Program 1 Press PROGRAM then use the...
Page 34: ...ow rotor must be at 0 RPM The audible tone that sounds when an error occurs cannot be disabled 1 To disable the key press ENTER then press 2 3 6 The words Disable KEY beeper appear on the display 2 To...
Page 35: ...caused by incorrect input or certain operating conditions can be cleared by pressing CE and following the instructions in the message Diagnostic messages that result from abnormal operating conditions...
Page 36: ...temperature setting by more than 4 C but less than 8 C after equilibration Run continues the message is informational Press CE to clear message C2 Rotor temp exceeds 8C above set call Service Rotor t...
Page 37: ...le Rotors in CHAPTER 1 Check that magnets are in place on rotor bottom Press CE to clear the message Call Beckman Coulter Field Service S1 System error call Service There is a problem with the system...
Page 38: ...g horizontal position which can cause imbalance Press CE to clear message Rotor tie down is not properly fastened Run shuts down with maximum brake Make sure rotor lid is properly aligned and tie down...
Page 39: ...the rotor stops Power not on Plug in the power cord turn power on Source power failure See Retrieving Your Sample in Case of Power Failure below Displays are blank Power not on Plug in the power cord...
Page 40: ...ate the two joined release cords underneath the bottom center of the centrifuge not visible from the centrifuge front See Figure 3 1 4 Pull on the cords or insert a screwdriver through the two cords a...
Page 41: ...strument Care WARNING Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock and or mechanical injury Therefore turn the power off and disco...
Page 42: ...ants from drying on component surfaces 1 To prevent accumulations of sample dust and or glass particles from broken sample tubes keep the chamber clean and dry by frequent wiping with a cloth or paper...
Page 43: ...baking enamel Ethanol 70 may be used on this surface See Chemical Resistances for chemical compatibilities of centrifuge and accessory materials While Beckman Coulter has tested ethanol 70 and found...
Page 44: ...d under Specifications in CHAPTER 1 Supply List Contact Beckman Coulter Sales or visit www beckman com for information about ordering parts supplies and publications For your convenience a partial lis...
Page 45: ...Then with the help of additional person s and or equipment move the centrifuge from the pallet to its final position 1 Select a location away from heat producing laboratory equipment with sufficient v...
Page 46: ...l Requirements To reduce the risk of electrical shock this centrifuge comes with a 2 5 m 8 ft three wire electrical cord attached to the power connector at the rear of the instrument and plug to conne...
Page 47: ...m the main power source in case of a malfunction Securing the Centrifuge to the Bench Allegra X 14 series centrifuges are certified to meet the centrifuge safety standards To meet these requirements t...
Page 48: ...t be plugged in and the power switch turned to on position I before the door can be opened We recommend that you make a test run to ensure that the centrifuge is in proper operating condition followin...
Page 49: ...f time shall be determined solely by Beckman Coulter Replacement Any product claimed to be defective must if requested by Beckman Coulter be returned to the factory transportation charges prepaid and...
Page 50: ...PN B07493AD Warranty 2 Beckman Coulter Inc Allegra X 14 Series Centrifuge Warranty...
Page 51: ...sem Material Riesgo biol gico Risque biologiqu Pericolo biologico Material infeccioso potencial On power Ein Netzverbindung Encendido Marche mise sous tension Acceso sotto tensione Fora o poder Off po...
Page 52: ...en Sie Anwendungsvorschriften Consulte las instrucciones para el uso Consultez les instructions pour l usage Consulti le istruzioni per uso Consulte instru es para o uso English Deutsch Espa ol Fran a...
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Page 54: ...tances for Beckman Coulter Centrifugation Products PN IN 175 Available in hard copy or electronic pdf by request Available at www beckmancoulter com RPM to RCF Conversion Chart when Running Multiwell...
