BD FACSFlow Supply System User’s Guide
36
Flushing the system
To flush the system:
1. Fill the stainless steel container with 2 L of 10% bleach
solution (or full-strength BD FACSClean solution).
2. Open the roller clamp on the fluidics line for about 5 seconds
and drain the fluid into a beaker. This runs bleach up into the
fluid line.
3. Confirm that the HTS sample coupler is connected.
4. Place the cytometer in RUN mode.
5. From the software menu, select
HTS > Prime
, then repeat the
prime.
6. Select
HTS > Monthly Cleaning.
The
Monthly Clean
dialog is displayed.
7. Click
Continue
.
A dialog is displayed while cleaning is in progress. Cleaning
can take up to 30 minutes.
8. Remove the stainless steel container and fill it with DI water.
9. Reinstall the stainless steel container and click
Continue
.
10. Click
OK
when monthly cleaning is complete.
11. Place the cytometer in STNDBY mode, disconnect the air line
at the stainless steel container, and vent the stainless steel
container.
12. Reconnect the air line to the stainless steel container.
13. At the cytometer, disconnect the air and fluid lines coming
from the stainless steel container.
Note:
Make sure to rinse any bleach out of the stainless steel
container when the procedure is completed to avoid corrosion.
Caution!
Confirm that the sheath filter is not in the
fluid line. Bleach will damage the filter.
