Baker SG403A Operator'S Manual Download

Page: 1 / 65

Document: 333D012G

5-18-2007

AKER

OMPANY

OPERATOR’S MANUAL

SterilGARD

III Advanceº

Class II, Type A2

Biosafety Cabinet

MODEL

SG403A / SG603A

This manual includes information for installation, operation, maintenance and spare parts. We
recommend that it be kept near the cabinet for ready reference.

Summary of Contents for SG403A

Page 1: ...PERATOR S MANUAL SterilGARD III Advanceº Class II Type A2 Biosafety Cabinet MODEL SG403A SG603A This manual includes information for installation operation maintenance and spare parts We recommend that it be kept near the cabinet for ready reference ...

Page 2: ...r techniques requiring a contamination free atmosphere In addition to the high quality you expect from all Baker equipment this model has been ergonomically designed to provide the lab user with many exciting design features The ergonomic design will help prevent repetitive motion injury reduce fatigue and lab accidents and enhance productivity The adequacy of a cabinet for user safety should be d...

Page 3: ...ht 9 Electrical Specifications 9 Environmental Conditions 10 Symbols and Terminology 10 II PREPARING THE SterilGARD III Advanceº FOR USE 11 Checking the Cabinet Upon Arrival 11 Location Within the Laboratory 11 Installing the Cabinet 11 Exhausting into the Room 12 Connecting the Exhaust for Ducting Outdoors 12 Final Connections and Tests 14 III PROPER CABINET USE 15 Operator Controls 15 Fig 2 Oper...

Page 4: ... 35 Dimensional Drawing Standard Configuration SG403A 37 Dimensional Drawing Standard Configuration SG603A 38 Cable Port Illustration 39 Ladder Schematic SG403A 41 Ladder Schematic SG603A 42 Stand Assembly Leg Extension Procedure 43 Channel Stand Installation or Removal Procedure 47 Replacing Fluorescent and Ultraviolet Lamps 48 Installation of Exhaust Transition Systems for SterilGARD III Cabinet...

Page 5: ...e work surface Some of the air enters the rear work area perforation while the remainder of the air enters the front work surface perforation The air is pulled through the drain pan area up the rear and side wall plenums to the cabinet blower The air is then pushed into the positive pressure plenum At that point most of the air is pushed through the down flow HEPA filter while the remainder is exh...

Page 6: ...n area Adjustable heights stand with leg levelers Down flow filter diffuser protector Membrane switch control pad GFCI protected duplex 2 Fluorescent lamp Petcock on right hand wall Padded armrest CABINET BALANCING DAMPER CONTROL EXHAUST HEPA FILTER PROTECTOR DRESS PANELS EXHAUST HEPA FILTER DOWN FLOW PLENUM DOWN FLOW HEPA FILTER REMOVABLE WORK SURFACE AND SUPPORTS FLUORESCENT LAMP LIGHT CANOPY SL...

Page 7: ...s the total air volume being moved inside the cabinet The internal cabinet balancing damper compensates for down flow and exhaust imbalances due to pressure drop differences between the filters when they are replaced Tested HEPA filters All filters in the cabinet are scan tested HEPA filters They are 99 99 effective on particles of 0 3 micron size Each filter is leak checked after installation to ...

Page 8: ...n Viewscreen The cabinet s 1 4 6 35mm safety plate glass sliding viewscreen may be opened to 19 3 8 492 1mm for placing of large items in the work area and maybe fully closed for system shutdown or UV light operation Work area lighting The work area is illuminated by two external fluorescent lamps which provide 100 foot candles of light at the work surface Electronic ballast The SterilGARD III fea...

Page 9: ...ter servicing or replacement Cable ports Optional A cable port is located in the cabinet s left and right side walls It provides a way of introducing power and data cables or siphoning tubes into the work area of the cabinet without having to go through the front viewscreen access opening A plug is provided for each port when the port is not being used or the cabinet is being decontaminated ...

Page 10: ...rnished with one 14 power cord with 20 amp plug type NEMA 5 20P The power cord is the Main Disconnect device for the unit The unit should be positioned in a manner that allows easy access to the power cord connection to the electrical utility The cabinet is provided with Fluorescent lighting The cabinet is provided with two GFCI protected 115V AC Duplex outlets The outlet s on this circuit are pro...

Page 11: ...g linearly to 50 at 40 C Main Supply voltage 115V 10 V AC Transient over voltage according to Installation Category OVERVOLTAGE CATEGORIES II per UL 61010 1 Annex J Pollution Degree 2 Symbols and Terminology Protective Earth Any terminal intended for connection to external protective conductor for protection against electric shock in case of a fault General Caution Refer to instruction manual for ...

Page 12: ...k area or escape from it for more information contact Baker If the cabinet exhausts air into the laboratory instead of venting to the outside it is important that there is adequate space between the top of the cabinet and the ceiling A solid ceiling located too close to the exhaust filter will restrict the air and limit the intake velocity The exhaust filter guard an inverted V shaped perforated s...

Page 13: ...culates and not gases 3 Keep the exhaust filter guard on the cabinet It will help protect the filter from objects being dropped on it and keep the cabinet spaced from the ceiling or other objects so it can exhaust properly Cabinet Model Sash Height Inches Exhaust Flow Range Approximate CFM SG403A 8 203 2mm 256 282 121 133 L sec 10 254mm 318 352 150 166 L sec SG603A 8 203 2mm 388 428 183 202 L sec ...

Page 14: ...lass II Type A2 Biosafety cabinets A Hard Exhaust Connection HEC requires a 10 254mm diameter connection between the cabinet and the building exhaust duct with no air gaps It mounts directly over the exhaust filter and includes an access panel for leak checking the exhaust filter We recommend having an airtight damper ATD to seal the cabinet for decontamination Cabinet Model Sash Height Inches Exh...

Page 15: ...ng with the interior work area NOTE The Fluorescent Light will not come on unless the blower switch is on The Fluorescent Light and UV Light are interlocked so they cannot operate simultaneously 6 If your cabinet has been purchased with the optional Ultraviolet Light UV lower the viewscreen to its fully closed position and turn the UV light on to make sure it is operational NOTE The UV light optio...

Page 16: ...o This person should monitor the cabinet and its operating personnel at regular intervals to see that it is being used correctly In order to keep the interior work area clean and free of particulates all Baker biosafety cabinets are designed for continuous operation If the blower is turned off the work area will become contaminated with room air Therefore it is recommended that the blower be left ...

Page 17: ...ced at the allowable opening of 8 or 10 203 2mm or 254mm The sash alarm will be activated whenever the viewscreen is raised above or lowered below this level To mute the audible alarm press the alarm reset button The indicator light located below the switch will continue to flash After five minutes if the conditions persist the alarm will sound again to remind you to reposition the viewscreen to i...

Page 18: ... the drain coupling is capped 4 Wipe down the interior area of the cabinet with surface disinfectant NOTE Some disinfectants such as bleach or iodine may corrode or stain the steel surfaces Good practice is to thoroughly clean the surface afterward with a detergent and rinse with sterile water to prevent corrosion 5 Place all materials to be used for the next procedure inside the cabinet on the so...

Page 19: ... The operating position of the sash provides an 8 or 10 203 2 or 254mm access opening This restricted opening permits optimum operating conditions for the cabinet Because operators will not all be the same height it is suggested that the operator use a chair that may be adjusted for height 9 After a procedure has been completed all equipment that has been in contact with the research agent should ...

Page 20: ...rence Cable Port Illustration in appendix Decontamination Whenever maintenance service or repairs are needed in a contaminated area of your cabinet the cabinet must first be decontaminated with an appropriate agent The National Institute of Health National Cancer Institute and the Centers for Disease Control have all recommended the use of formaldehyde gas for most microbiological agents Its appli...

Page 21: ...ght of ammonium bicarbonate than paraformaldehyde This will be used later in step 13 to neutralize the formaldehyde gas 7 This step is recommended Place spore strips inside the cabinet to confirm that the decontamination process has been successful 8 If the cabinet is vented to the room use a sheet of plastic and seal the front access and exhaust port openings These openings should be sealed such ...

Page 22: ...should always follow disinfection and cleaning Disinfect the work surface before and after every procedure 1 Disinfect surfaces of all equipment used 2 Remove all items from the inside of the cabinet 3 Place all items that may have come in contact with the agent s such as used pipettes in a plastic bag or other suitable container 4 Disinfect the entire inside surface of the cabinet For additional ...

Page 23: ... definition because theoretical studies have shown that filtration efficiency should be at a minimum for particles of this diameter with efficiency increasing for particles either larger or smaller Experiments with various viruses and microbial agents have proven the effectiveness of HEPA filters Contact The Baker Company for more information It must be pointed out that the HEPA filter is not inte...

Page 24: ...ular cabinet model Alternate testing procedures can be found in the NSF International Standard No 49 The Airflow Balance WARNING Only qualified technicians should perform this procedure The airflow balance which is set at the factory provides your cabinet with the proper air volume and velocity control to minimize leakage of airborne contamination either into or out of the work area In order to du...

Page 25: ... exceeding NSF International Standard 49 allowable leak rates Since filters may become damaged in transport we recommend that the filters be re tested by qualified technicians before the cabinet is used The filters should also be leak tested at prescribed intervals as specified by an industrial hygienist safety officer or other qualified person NSF International details the steps for performing th...

Page 26: ...ls that fall through the perforated grilles can be removed through the drain valve after proper decontamination To wash the drain pan under the work surface lift up surface decontaminate and remove the solid work surface and work surface supports This provides unobstructed access to the drain pan for easy cleaning Remember that this area must be assumed to have contamination so use caution in the ...

Page 27: ...d It is advisable to seal the contaminated side of the filter by taping a plastic sheet or cardboard over the face before removal This should minimize the number of particles shaken loose from the filter Once removed the filter should immediately be sealed in a chemical hazard bag and then disposed of safely in accordance with environmental regulations After filter replacement has been completed t...

Page 28: ... support cables on each end are secured 5 Remove the two screws at the top and loosen the two lower lock nuts holding the sloped dress panel Lift the panel off the cabinet 6 Loosen the four lock nuts holding the electrical mounting board and carefully lift the board off Lay it face down inside the light canopy No electrical connections should need to be disconnected 7 Loosen each of eight 10 3 8 h...

Page 29: ...ions at a time until the filter gasket is compressed approximately 1 8 3 2mm 5 Reassemble the cabinet following the previous instructions in reverse order IMPORTANT When reinstalling the front seal panel first tighten each 20 hex cap screw until the bolt seal washer is engaged with the panel Next turn each bolt 1 to 2 revolutions until the panel gasket is evenly compressed approximately half way D...

Page 30: ...mately 1 8 3 2mm 5 Tighten the two 2 hex head down flow plenum clamping bolts alternating two or three revolutions at a time until the filter gasket is compressed approximately 1 8 3 2mm 6 Reassemble the cabinet following the previous instructions in reverse order IMPORTANT When reinstalling the front seal panel first tighten each 20 hex cap screw until the bolt seal washer is engaged with the pan...

Page 31: ...peed control is turned up to maximum voltage and the airflow is still low decontaminate the cabinet and replace the filters If there is no down flow within the work area 1 Make sure the cabinet is plugged in and the blower switch is turned on The Green indicator below the button should be lighted 2 Check the wiring connections between the electrical panel and the cabinet to see if they are connect...

Page 32: ...osed You can silence the audible alarm for five 5 minutes by pressing the Alarm Mute button The indicator will continue to flash until the window is set in the correct position After five 5 minutes the alarm will sound again You may press the Alarm Mute button again 2 Open the electrical panel and check to see if the proximity sensor cables are connected If the Fluorescent Light doesn t work 1 The...

Page 33: ...07 32 A lower reading than what was originally recorded on the cabinets test report could be an indication of the following 1 Blocked or partially obstructed filters 2 Work surface has been removed 3 Incorrect airflow balancing ...

Page 34: ...off the cabinet Store in a safe place 6 Removal of sliding viewscreen A Lift the viewscreen up to its maximum height B Place 8 32x 3 4 long truss head screw in tapped hole at top of sash brackets under each viewscreen cable tab Leave the screws out approximately 3 8 to 1 2 This will support the viewscreen while the cables are being disconnected C Remove the 8 32 hex head screw that holds the cable...

Page 35: ...Document 333D012G 5 18 2007 34 Appendix ...

Page 36: ...58 39658 Transformer 34327 34327 Relay solid state 1855167 1855167 Terminal block 40450 40450 Proximity switch Fixed wire Quick disconnect 40647 40671 40647 40671 Right Angle Micro Cable 40672 40672 Membrane Switch Controller MSC board 40702 40702 Membrane switch Overlay 42301 42301 Plug Power cord NEMA 5 20P 32897 32897 Circuit breaker Main 40449 40449 Self resetting breaker Outlets 34331 34340 G...

Page 37: ...rk surface 333X104 336X106 Work surface support 333P706 333P706 Down flow diffuser 333P709 336P709 Viewscreen assembly 333A310 336A310 Viewscreen frame assembly 333A311 336A311 Viewscreen track right hand 333A303 333A303 Viewscreen track left hand 333A304 333A304 Front Panel 333X604 336X604 Sash Balance 38014 38172 38173 Side Panel LH POBM 333X650 333X650 Side Panel RH POBM 333X651 333X651 ...

Page 38: ...Document 333D012G 5 18 2007 37 Dimensional Drawing Standard Configuration SG403A ...

Page 39: ...Document 333D012G 5 18 2007 38 Dimensional Drawing Standard Configuration SG603A ...

Page 40: ...T GASKET PART 41126 CABLE PORT RING PART 190P728 4 8 32 X 5 16 WELD STUD WITH HEX CAP NUT LOCK WASHER FLAT WASHER PART S 10758 10553 11307 11279 4 8 32 X 5 16 WELD STUD WITH HEX CAP NUT LOCK WASHER FLAT WASHER PART S 10758 10553 11307 11279 CABLE PORT RING PART 190P728 CABLE PORT GASKET PART 41126 EXTERIOR SEAL PANEL REMOVABLE INNER WALL PANEL NCB D4 PART 195P612 NCB D6 PART 191P629 ...

Page 41: ...Document 333D012G 5 18 2007 40 Interior View Exterior View Cable port plug not shown ...

Page 42: ...Document 333D012G 5 18 2007 41 Ladder Schematic SG403A ...

Page 43: ...Document 333D012G 5 18 2007 42 Ladder Schematic SG603A ...

Page 44: ...er option 30 1 8 765mm and 38 5 8 981mm Adjustable Leg Leveler Option 30 762mm and 36 914mm Caster Option Remove the following parts from the hardware box shipped with the unit Check the quantities with the list below Item No Description Qty 1 Adjustable Leg Leveler or Caster Option 4 2 3 8 16 x 1 Hex head bolt 8 3 3 8 Lock washer 8 4 3 8 Flat washer 8 5 7 16 Plug button 8 2 3 4 5 1 ...

Page 45: ...ide the unit into its destination room before going further with the assembly installation Installing the Adjustable Leg Leveler or Caster Option 3 Lift one end of the unit stand assembly up a minimum of 7 178mm and block in position See illustration below 4 Screw in two of the Adjustable Leg Leveler or Castors Item 1 Screw in by hand as tight as you can then remove the blocking 1 ...

Page 46: ...at steps 3 and 4 for the other end of the unit Raising the Unit to 30 1 8 765mm Work Surface Height Adjustable Leg Leveler Option Raising the Unit to 30 762mm Work Surface Height Caster Option 6 Lift one end of the unit up a minimum of 5 127mm and block in position 7 Unscrew the bolts One per leg that hold the legs in the shipping position 8 Slide the leg out of the stand until the holes line up a...

Page 47: ...13 Lift one end of the unit up a minimum of 7 178mm and block in position 14 Unscrew the bolts Two per leg that hold the legs in the current work surface height position 15 Slide the leg out of the stand until the holes line up at the next position 16 Bolt the leg in position with items 2 3 and 4 Two bolts per leg 17 Repeat steps 14 through 16 for the other leg then remove the blocking 18 Repeat s...

Page 48: ...ly in the upright position so that the leg levelers are now resting on the floor The stainless steel legs can be adjusted to your desired height requirement This is done by removing and replacing the 2 3 8 bolts in each leg Unused rivnuts in the legs will be plugged with the 8 chrome dots If the cabinet is not in its permanent location you may want to perform the leg adjustment after step 7 5 Lift...

Page 49: ...inge the dress panel standoff located on the left side of the sloped dress panel down and lower the vertical panel on it While removing the thumbscrews support the light canopy with the other hand Lower the canopy until the restraint cables hold it in place 3 Disconnect the restraint cables from the face of the electrical mounting board at both ends of the light canopy Fig 2 Gently hinge the canop...

Page 50: ... properly Fig 3 6 Hinge light canopy up temporarily and press the light on off switch to verify that both lamps are working 7 Lower canopy halfway and re insert restraint cables on either end of electrical mounting board Close the canopy and install the thumbscrews finger tight 8 While lifting the vertical dress panel up slightly hinge the dress panel standoff up against the sloped dress panel Gen...

Page 51: ...the light canopy After five minutes the alarm will sound again and you may press the reset button if more time is needed for lamp replacement 2 Using both hands grasp the UV lamp carefully near each end and move it approximately 13mm to the right side of the cabinet until the UV lamp is released from the lamp socket on the left side Carefully remove lamp from cabinet and dispose of it properly 3 I...

Page 52: ...wing packing lists and illustrations Item Description Qty SG403A Qty SG 603A 1 Exhaust transition 1 1 2 Exhaust filter access cover closed face 1 1 3 20 x Washer head bolt 4 6 4 Seal washer 6 8 5 20 x 1 Hex head bolt 4 4 6 Flat washer 6 6 7 Lock washer 6 6 8 20 Double ended transition mounting bolt 2 2 9 20 Hex nut 2 2 10 Exhaust transition mounting clip 2 2 11 Air tight damper assembly ATD 1 1 12...

Page 53: ...nded transition mounting bolt 2 2 9 20 Hex nut 2 2 10 Exhaust transition mounting clip 2 2 11 Air tight damper assembly ATD 1 1 12 Short exhaust collar Optional 1 1 13 8 32 Hex nut 4 4 14 8 Flat washer 4 4 15 8 Lock washer 4 4 16 Exhaust transition gasket x 8 3 ft 10 3 ft 17 Access cover gasket x 6 2 ft 8 3 ft 18 Decon Seal Box Assembly Optional 1 1 19 8 32 Hex Head Screw 8 8 Packing List for Cano...

Page 54: ...403A 8 203 2mm 269 127 L sec 0 06 14 9 Pa 11 1 2 5 5 16 292 135mm 10 254mm SG403A HEC403A 10 254mm 335 158 L sec 0 08 19 9 Pa 11 1 2 5 5 16 292 135mm 10 254mm SG603A HEC603A 8 203 2mm 408 193 L sec 0 10 24 9 Pa 11 1 2 5 5 16 292 135mm 10 254mm SG603A HEC603A 10 254mm 510 241 L sec 0 15 37 4 Pa 11 1 2 5 5 16 292 135mm 10 254mm Exhaust requirements for Hard Exhaust Connection HEC All exhaust flow ra...

Page 55: ...haust Connection CEC All exhaust flow rates are based on a nominal 105 fpm 0 533 m sec intake velocity When building exhaust is off cabinet running If the cabinet intake varies from the nominal 105 fpm 0 533 m sec adjust the building exhaust flow rate to a minimum of 20 over the intake airflow INSTALLATION INSTRUCTIONS FOR HEC Reference Figures 1 2 NOTE Cabinets shipped with exhaust transition ass...

Page 56: ...haust filter protector assembly located over the exhaust filter opening by removing two 2 8 32 locking nuts and washers This assembly will no longer be required 2 Remove the exhaust filter access panel Item 2 from the front of the transition by removing four 4 bolts with seal washers Items 3 4 Detail A 3 Remove the two 2 top panel mounting bolts located in front of the exhaust filter opening Insta...

Page 57: ...tem 12 and gasket to the eight 8 studs located at the top of the transition using 8 hardware Items 13 14 15 Detail B With the CEC installed and connected to the building exhaust system the cabinet should be certified by a qualified technician to assure proper cabinet performance Figure 1 Type A2 cabinet Exhausted to the room Balancing Damper Control Mounting Hardware Exhaust filter protector ...

Page 58: ...nection HEC Items 3 4 Items 13 14 15 Items 5 6 7 Items 6 7 9 10 Gasket Item 18 Short Exhaust Collar Optional Item 12 ATD Assembly Optional Item 11 Exhaust Transition Items 1 16 Double ended transition mounting bolt and seal washer Items 4 8 Exhaust Filter Access Cover Items 2 17 Exhaust Filter ...

Page 59: ...bly Optional Item 11 External Damper Control Exhaust Transition Items 1 16 Double ended transition mounting bolt and seal washer Items 4 8 Exhaust Filter Access Cover Items 2 17 Items 3 4 Items 5 6 7 Items 6 7 9 10 Item 18 19 Exhaust Filter Gasket Item 18 Short Collar Optional Item 12 Decon Seal Box Optional Items 13 14 15 ...

Page 60: ...asses through the hole in the airgate approximately 3 32 2 38mm Tighten the retaining collar set screw carefully Do not over tighten Make sure not to rotate the probe while tightening 3 Remove the 8 32 hardware already fastened to the two weldstuds on top of the cabinet 4 Next grasp the Airgate and carefully lower it onto the top seal panel locating the keyhole contour onto the far left weld stud ...

Page 61: ...cating the keyhole contour onto the far left weld stud Insert the 8 32 locknut provided leaving a 1 16 gap between the airgate flange and the lower portion of the nut Once the Airgate has been installed carefully slide cardboard protector sheet to the right side of the exhaust filter and remove 4 Run the probe wiring thru the slot located on the left side of the front access opening Figure 3 Re in...

Page 62: ...the audible alarm Make sure the slide switch for the audible alarm is in the ON position Turning the alarm ADJUST screw clockwise will move the CFM alarm point up and counter clockwise will move the CFM alarm point down 2 Return the exhaust system to it proper flow rate and recheck cabinet intake velocity for HEC operation 3 Return the cabinet blower motor speed controller to it original voltage a...

Page 63: ...nt 333D012G 5 18 2007 62 Figure 2 Parts Included DETAIL B REMOVE ACCESS PANEL AIRGATE EXHAUST TRANSITION SLOT FOR PROBE WIRING 8 32 LOCKNUT B AFM w Probe AirGate 8 32 Locknut Cardboard Protector Velcro Strip ...

Page 64: ...combinations thereof which contains or are contaminated with viable hazardous agents For the purpose of this subparagraph the term biological hazard or biohazard shall include only those infectious agents presenting a risk or potential risk to the well being of man The biohazard symbol shall be designed and proportioned as illustrated in figure J 9 We have enclosed a sign featuring the Biohazard S...

Page 65: ...Maine furnish without charge repairs to or replacement parts or equipment which proved defective in material or workmanship No claim may be made for any incidental or consequential damages THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES EXPRESSED OR IMPLIED INCLUDING ANY IMPLIED WARRANTY OF MERCHANTIBILITY OR FITNESS FOR A PARTICULAR PURPOSE UNLESS OTHERWISE AGREED IN WRITING SIGNED BY THE BAKER ...

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