Chapter 5 Trouble shooting guide
43
No solvent flow through degasser
Possible cause
Remedy
Air in pump head
Prime/ purge the pump head
Blocked 0.2 µm in-line filter
Replace filter
Blocked degasser solvent
port caused by unfiltered or
buffered mobile phases
Use a different degasser channel or
flush/back flush the channel with water or
other appropriate solvent (MeOH, iso-pro-
panol. Note: draw the solvent through the
channel to dissolve salts or remove parti-
cles.
Regular baseline oscillations
Possible cause
Remedy
Malfunctioning pump (regular
pattern)
Check pump (seals, valves)
Back pressure too low for
pulse damper
For optimal dampening of flow pulsations a
backpressure of at least (7 MPa = 70 bar
=1000 psi) is needed. Use backpressure
regulator or restriction capillary
between
pulsdemper and injector
to increase pres-
sure
Pulse damper defect (internal
membrane ruptured)
Contact your local supplier for service
Leakage of mobile phase via OR 100 drain (bottom plate)
Possible cause
Remedy
Internal leakage of degasser
vacuum chambers or tubing
Contact your local supplier for service
Internal leakage of pulse
damper
Contact your local supplier for service
When trouble shooting is not successful, contact supplier.
