Device User’s Guide
4.
Instrument setting for absorbance / transmittance measurement
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4.
Instrument setting for absorbance/transmittance measurement
Absorbance is calculated from the original light source spectrum and the spectrum when
the irradiation light of the light source passes through the sample to be measured.
Therefore, to measure the absorbance, first measure the spectrum of the light source,
then set the sample to be measured and measure the light that passed through them.
When the measurement is completed, the program automatically calculates the
absorbance spectrum.
The transmittance is also calculated from the spectrum of the light source and the
spectrum after it has passed through the sample to be measured. When the
measurement is completed, the program automatically calculates the transmittance
spectrum.
Example: 1. Using cuvette
Set the cuvette containing the sample (gas or liquid) and measure the absorbance and
transmittance. Also, by switching to the concentration mode, quantitative analysis of
concentration is also possible.
Configuration example
PC (sold separately)
SEC2021 Spectrometer
SEC2022 Deuterium halogen light source
SEC2023 Cuvette holder
SEC2024 Platform
Platform screw
Fiber collimator
SMA905 adaptor for light shielding
Plastic cuvette
Figure 4-1: Absorbance/Transmittance measurement example using cuvette
