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EP - culture ….. This menu is provided for recording the evoked response (the spike) in a dissociated neural culture (cultured neural
network). A session consisting of 0.1 s data acquisition and 4.9 s waiting time is repeated 100 times. Max input is
set at 10,000 uV. A low frequency component may be generated at baseline by LFP (local field potential) caused by
spontaneous activity, and filtering is applied to bandwidths 100 to 10,000 Hz to allow for the easy observation of a
spike. Whether a spike is evoked or not by a default stimulation pulse (0.1 ms width, -20 uA) at any electrode in a
constant delay time from stimulation artifact is observed while switching the stimulation electrode. When no
evoked spike is observed at any stimulation electrode, repeat the same procedure with the stimulation intensity
increased (e.g., -30 uA).
EP - IO curve ….. In this menu, the input-output relationship (the input-output curve of the evoked potentials relative to the
stimulation intensity) is recorded in a brain slice. The data acquisition condition is almost the same as the EP-
baseline, but the stimulation intensity is increased by 1 uA for every 1 repeat because by rep # it is enabled. The
number of repeats is set to 90 because the max stimulation intensity is 100 uA. The data acquisition is stopped
manually when a saturation response is obtained.
ERP ….. This menu is provided for recording the effective refractory period by premature stimulation in a dissociated
cardiomyocytes. Electric stimulation with width 0.6 ms, intensity -10 uA and basic cycle length (interval between pulse)
1,000 ms is applied 10 times, followed by the 11th electric stimulation with a shorter cycle length of 900 ms.
LTP - HFS ….. This menu is provided for LTP induction by tetanus stimulation. The data acquisition condition is almost the same as
the EP - baseline, but tetanus stimulation with 10 ms interval (100 Hz) for 1 s (100 stimulation) is set in the Manual
tab. The default stimulation intensity is -20 uA. To change the value to a value defined based on the result of the
input-output relationship, enter the difference between the default value and the new value in Increase intensity, and
click on the in all units button to increase/decrease the stimulation intensity for the stimulation patterns as a whole.
LTP - TBS ….. This menu is provided for LTP induction by θ burst stimulation. The data acquisition condition is almost the same as
the EP - baseline, but 4 pulse stimulation of 100 Hz is set at 200 ms (5 Hz) interval for 2 s (10 times) in the Manual
tab. The default stimulation intensity is -20 uA. To change the value to a value defined based on the result of the
input-output relationship, enter the difference between the default value and the new value in Increase intensity, and
click on the in all units button to increase/decrease the stimulation intensity for the stimulation patterns as a whole.
Spontaneous ….. Data in the bandwidths 1 to 10,000 Hz is acquired with Max input 10,000 uV. Data for continuous 20 m interval is
acquired with no stimulation.
Straight pacing ….. This menu is provided for recording the paced responses in a fixed cycle for a cardiac culture. Repeat all the
time is enabled and pacing is applied continuously at stimulation width 0.6 ms, stimulation intensity -10 uA, and
cycle length (interval between pulses) 1,000 ms. To change the cycle length, edit the value in Post.
Note: When any menu is called up from EASY SETTINGS and switched to Custom, the Custom takes over the acquisition condition
of that menu.
4.3. Set data file output –
FILE OUTPUT subpanel
When data acquisition is executed by the record button, a data file is generated after the data acquisition. The folder to output
and the file name are specified in the field shown in the figure below:
In RECORD mode, the output format of the data file cannot be specified and the file is output in a unique .endat format. The file
name is automatically assigned in the format “Year Month Date_####h##m##s(_added text).endat.”
Must be specified. If no folder is
specified before data acquisition, a
prompt window appears.
Optional item. Text to be added to
the file name is entered.
The format of the data file to be
output is specified. The format
cannot be specified for recording
(it can be specified for replay only).
Summary of Contents for MED64-Entry
Page 1: ...MED64 Entry Manual ...
