MED64-Entry Manual
Page 1: ...MED64 Entry Manual ...
Page 2: ...g the data acquisition schedule RECORD subpanel 10 4 2 Creating the stimulation pattern STIMULATION subpanel and STIMULATION PATTERN tabs 11 4 3 Set data file output FILE OUTPUT subpanel 13 4 4 SIGNAL WAVEFORM panel 14 4 5 BASELINE STABILITY panel 14 4 6 Replaying data file output function of REPLAY mode 16 5 Abnormal noise 17 5 1 Noise check point 17 5 2 Noise relation to installation 17 5 3 Nois...
Page 3: ...ack or carbon nanotube with impedance 7 to 10 kΩ the lowest impedance among similar products on the market The system is substantially free from external noise thanks to its low impedance electrodes It needs no special environment facility for electrophysiological experiment such as a shield box and it can be placed on a laboratory desk for stable daily measurement with no need for noise eliminati...
Page 4: ...onnector It is connected to a connector terminal via the 68 pin SCSI cable SIGNAL GND A ground terminal to connect a lead for grounding PCIe 6343 Output terminals to the data acquisition device PCIe 6343 National Instruments Corporation They are connected to a VDHCI terminals of the data acquisition device on the back of the data acquisition PC via multifunction cables 192061C 02 National Instrume...
Page 5: ...ce When the MED64 Entry is set up on a desk against a wall make space for the cord between the wall and the desk and place the adaptor on the floor in order to place the power adaptor at a distance from the amplifier and other components the desk must not be fixed to the wall Alternatively a space is necessary to ensure sufficient distance between the MED64 Entry and the power adaptor 3 1 Connecti...
Page 6: ...ion PC System the desktop PC and the display monitor and one additional power source is necessary to use the MED Thermo connector when it is used Connect all the cords to one power strip connected to a wall outlet with the ground terminal do not use an outlet on the desktop rack because it is often not grounded properly Do not connect devices that are not necessary for the MED64 Entry to the power...
Page 7: ...lifier using the 68 pin SCSI cable Connect the lead of the 68 pin SCSI cable to the SIGNAL GND terminal of the MED64 Entry Amplifier Note1 The 68 pin SCSI cable is wound with a conductive cloth tape and an uncoated lead is wound on it If the other side of the lead is free the conductive cloth tape may serve as an antenna that collects electric noise and may cause noise Make sure that the other sid...
Page 8: ...tom is assigned to ch 64 The orientation of the MED Probe is important because the electrode number of the MED Probe is defined by the contact pins of the MED Connector Correspondence between the contact pin of the MED Connector and the electrode number correspondence from the contact surface side with the output terminal on the right left panel and correspondence with the MED probe terminal right...
Page 9: ...n a sheet of aluminum foil is placed under the MED Connector even if the baseline noise level is within an acceptable range Without grounding the aluminum foil serves as an antenna to collect electric noise and may generate unexpected noise MED64 Entry after setup In this example the PC is placed on the right side 3 4 5 Preparation for the noise check attaching the MED Probe Loosen the fixation sc...
Page 10: ...s For the details of operating the MED16 refer to p 9 Data acquisition by the control software Refer to the relevant application note for the preparation of specimens used for the experiment Normal baseline noise 3 4 7 Maintenance of the MED Connector A printed circuit board onto which 68 contact pins contacting the terminal of the MED probe are soldered is fixed by screws to the top unit of the M...
Page 11: ...m the signal waveform it calculates a measure that indicates the stability of the activity or response of the biological specimen and it displays it as a time chart BASELINE STABILITY has three modes Spike EP and ISI In Spike mode the calculation is based on the frequency of spike detection in EP mode the calculation is based on amplitude value of the evoked potential and in ISI mode the calculati...
Page 12: ...running background such as anti virus software Do not connect to the Internet because it may place an unexpected load due to the communication and cause it to freeze or to force a shutdown Note2 In this document data acquisition with data file output is called record 4 1 Setting the data acquisition schedule RECORD subpanel The data acquisition schedule consists of 1 or more data acquisition sessi...
Page 13: ...ion pattern of Step 1 is applied and so on see the right panel of the figure below Because the MED64 Entry Amplifier has 2 terminals S1 and S2 for stimulation output the stimulation pattern is applied to up to 2 electrodes in one Step Schematic drawing of data acquisition and stimulation pattern In this example a stimulus is applied to 1 electrode in Step 1 and a different stimulus is applied to t...
Page 14: ...y use the specific data acquisition condition defined by themselves without having to define it again Note In menus other than Custom the condition defined by the user cannot be stored Default values are called up every time the software is launched EP baseline This menu is provided for recording the evoked response the evoked potentials in a brain slice A session consisting of 0 1 s data acquisit...
Page 15: ...ult of the input output relationship enter the difference between the default value and the new value in Increase intensity and click on the in all units button to increase decrease the stimulation intensity for the stimulation patterns as a whole LTP TBS This menu is provided for LTP induction by θ burst stimulation The data acquisition condition is almost the same as the EP baseline but 4 pulse ...
Page 16: ...omatically adjusted in 2 5 s increments for each electrode based on the maximum voltage value Copy Image copies the panel image onto the clipboard In Preferences Line Color customizes the color of data points Background Color customizes the background color of the chart and Grid Color customizes the color of the auxiliary lines By selecting Default the changes are returned to the default values 4 ...
Page 17: ...n the threshold is on the positive side and the cross point for falling when the threshold is on the negative side The same as in the SIGNAL WAVEFORM panel a menu to change the scope to display on an axis appears by right clicking on the signal waveform chart The value specified on the X axis indicates the maximum value s on the X axis For the Y axis click the axis label of the extreme value to ch...
Page 18: ...e record button The MED16 has limited functions for data analysis in order to reduce the load during operations For data analysis consider using the MED64 Offline Toolkit or the separately sold MED64 Burstscope and MED64 Peakmap that are compatible with the endat format Refer to the product manuals of each product for further details Click to select a data file to replay When the file name appears...
Page 19: ...stance and noise for which an electric shield is not effective but can be reduced by moving the noise source to a distant position The latter noise may be caused by a magnetic field generated by a device connected to the power cable power adaptor perfusion pump and water bath etc Move the responsible device to a position where the noise disappears or unplug the power cable of that device when MED6...
Page 20: ...eld occurs in particular electrodes A heater circuit or electromagnetic valve of the incubator operates and a magnetic field is generated inside the incubator An example of magnetic field noise caused by an incubator Interference occurs in particular electrodes the magnitude of effect depends on the position in the incubator and the noise disappears when the temperature is stabilized an example sc...
Page 21: ...clip The platinum wire is not stably fixed with a fixation screw it is prone to staggering A spike like noise occurs at several seconds interval A flat baseline noise is obtained when the peristaltic pump is turned off The inside of the dropper is completely hydrophilic with a lot of water dropping It is likely that the inside insulation is destroyed every time the perfusion solution drops and the...
Page 22: ...f the position does not move proceed to Procedure 2 and the subsequent procedure Procedure 2 poor contact of cable or lead If poor contact is detected in the output terminal of the MED Connector or the input output terminal of the MED64 Entry Amplifier disconnect the SCSI cable completely and connect it again Check whether the lead or alligator clip connected to the GND terminal has come off or no...
Page 23: ... resolution The resolution of signals acquired is selected by changing Max input maximum input voltage of the MED16 The maximum input voltage selected represents the upper limit of the amplitude of the potential acquired When 2000 µV is selected for example a signal exceeding 2000 µV is acquired as a 2000 µV signal The low level maximum input voltage means high resolution The resolution of amplitu...
Page 24: ...C Ta 25 C Heater Transistor 6 1 4 Data Acquisition PC System recommended specification any PC model with equivalent performance can be used Desktop PC Operating System Windows 10 Pro 64bit CPU Intel Core i7 6700K 4 Cores 4 00 GHz 8 MB Cache The latest high performance model when buying RAM 16 GB 8 GB 2 1st Disk Drive for OS 256 or over GB SSD 2nd Disk Drive for Data Strage 1 or over TB HDD Display...
Page 25: ...ectrode impedance is higher than 100 MΩ blue line in the figure below On the other hand a platinum black electrode can acquire the signal without attenuation because its impedance is lower than 100 MΩ Impedance relationship between electrode and MED64 Head Amplifier 6 2 2 Electric stimulation by the MED64 system Applying current to the micro electrode array The MED64 head amplifier has a 2 channel...
Page 26: ...ze the duration of the stimulation artifact Follow the instructions described below to minimize the width of the stimulation artifact 1 Apply a current drive biphasic pulse stimulation consisting of a negative and positive pulse with the same width When the biphasic pulse stimulation is applied charge and discharge at the electrode occur quickly On the other hand when a monophasic pulse stimulatio...
Page 27: ...ion current is charged in the electrical double layer capacity and causes voltage Vc see figure below When the voltage Vc exceeds 1 V the electrodes induce electrolysis and discharge H2 This causes a marked decrease in stimulation efficiency and a marked expansion of the stimulation artifact and it damages cells and the electrodes Therefore it is essential to prevent a decomposition voltage by sti...
Page 28: ... stimulation pattern the STIMULATION subpanel and STIMULATION PATTERN tabs Please note that in the amplifier about 5 seconds is necessary to cancel mute at the stimulation electrode recovery of baseline noise Therefore define an interval of not less than 5 seconds between traces 0 01 ms 0 1 ms 1 ms 10 ms 2 µA 20 µA 200 µA 2 mA Stimulus intensity pulse duration monophase ...
Page 29: ...rrors or from the programs or source code described in this document In no event shall the publisher or authors be liable for any direct or indirect damages resulting from the use of this document 2022 Alpha MED Scientific Inc All rights reserved Version 1 00 2022 5 13 Manufacturing Alpha MED Scientific Inc Saito Bio Incubator 209 7 7 15 Saito asagi Ibaraki Osaka 567 0085 Japan E mail support med6...
