Agilent Technologies Seahorse XFe, Operating Manual

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3 Basic Operation
Operational and assay guidelines for non-37 °C assays
Seahorse XFe Analyzer Operating Manual 29
Operational and assay guidelines for non-37 °C
assays
• For all non-37 °C operation, the XFe Analyzer must equilibrate overnight in
the required ambient temperature.
• If it is required to set up the XFe Analyzer in a cold room, avoid direct fan
sources.
• For all non-37 °C operation, the tray heater must remain ON . Do NOT turn the
tray heater OFF .
• For assay temperatures below 30 °C, hydrate the Sensor Cartridge in the dark
at room temperature.
• Prior to starting an assay, an additional 30 minutes of precalibration
equilibration time has been added to ensure temperature stability.
To adjust the Target Temperature (set point) using the up/down arrows, do the
following:
1 Click the Temperature widget. (See Figure 11 .)
The Tray Heater dialog box is displayed. (See Figure 12 .)
Figure 11. Temperature widget
Figure 12. Edit temperature settings (Tray Heater dialog box)