G4208A User’s Guide
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4
Running Multiple-Vial Analyses
Analyzing Multiple Vials Using Different Methods
Analyzing Multiple Vials Using Different Methods
This section describes how to set up a 50- vial analysis using three methods
which you have previously created called e.g. METH1, METH2 and METH3. For
example: METH1 and METH2 have the same analytical settings but differ in the
injection volume and stoptime values. METH3 uses a different temperature and
requires a wait time of 30 min for the LC system to equilibrate.
•
The first 20 vials are analyzed using METH1 with one injection per vial,
•
the next 20 vials are analyzed using METH2 with two injections per vial,
•
the last 10 vials are analyzed using METH3 with three injections per vial.
The vials are located in positions 1 to 50 of the autosampler tray.
The example assumes that the sequence table is empty. If the sequence table is
not empty, use the
Delete
button to delete all lines or use
File - New
.
1
Select the Sequence screen.
2
Move to line 1 and press
Insert.
3
Select
Method
and then move to METH1 and press
Load.
4
Move to the End of sequence list, select the
Wizard
button and enter:
5
Select
Done
to accept entries.
6
Move to the End of sequence list and select
Insert.
7
Select
Method
and then move to METH2 and press
Load.
8
Move to the End of sequence list, select the
Wizard
and enter:
9
Select
Done
to accept the entries.
NOTE
This way of combining methods is called Sequence.
Vial Range
1 – 20
#Inj.
1
Vial Range
21 – 40
#Inj.
2
