G4208A User’s Guide
110
3
Running an Isocratic Analysis
Preparing the LC System
Preparing the LC System
1
For the isocratic pump, fill the solvent bottle with the mixture of LC- grade
bidistilled water (35 %) and acetonitrile (65 %). For the binary or quaternary
pump, fill one solvent bottle with bidistilled water (channel A) and the other
with acetonitrile (channel B).
2
Turn on the detector lamp and pump via the
Control - System On/OFF
screen.
3
For the quaternary pump, turn on the degasser by pressing the line-power
switch.
4
Purge the pump.
5
Allow the detector at least 15 min to provide a stable baseline.
6
Transfer the contents of an Agilent Technologies isocratic standard sample
ampoule into a vial and seal the vial with a cap. Place the vial in position 1 of
the autosampler tray.
7
Pump the water/acetonitrile (35/65 %) mobile phase through the column for
10 min at a flow rate of 2 mL/min.
