Agilent Technologies 2200 TapeStation, User Manual

Page: 61 / 72

2200 TapeStation 61
Troubleshooting
6
Troubleshooting the Test Kit Manufacturers TestKitSuite.xml File
Table 9 Overview troubleshooting test kit manufacturers TestKitSuite.xml file
Image Issue Probable cause Solution
A warning sign has
appeared in the
result table next to
an expected
fragment and a
corresponding
warning sign has
appeared in an empty
region of the gel
image. The TestKitSuite.xml file, provided by the PCR Test
Kit Manufacturer, has the ability to flag certain
fragments as important. This then ensures that a
warning sign is presented when that fragment is
not detected.
In this instance, the internal control has not been
detected. Run the sample
again. If the issue
re-occurs, contact
the Test Kit
Manufacturer.
A warning sign has
appeared in the
result table next to
Unidentified and a
corresponding
warning sign has
appeared in the gel
image.
The
TestKitSuite.xml
file, provided by the PCR
Test Kit Manufacturer, is only looking for specific
fragments (usually pathogens) within the sample.
Known fragment sizes are used to set diagnostic
windows with a minimum and maximum
allowable size range within the gel. Fragments
appearing within these predetermined windows
generate a positive result. The detection of any
unexpected fragments that are sized out with
these predetermined windows are marked as
unidentified and presented with a warning sign in
the gel and result table.
Run the sample
again. If the issue
re-occurs, contact
the Test Kit
Manufacturer.