Agilent Technologies 1260 Infinity User Manual Download Page 39

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HPLC-Chip Cube Interface User’s Guide

Installation

Unpacking the Agilent G4240A HPLC-Chip MS Interface

Table 5

Delivery Checklist for the G4240 HPLC-Chip MS Interface

Description

Quantity

Part Number

HPLC-Chip/MS Cube

G4240-64000

Power cord, local (matching the sockets of your country or region)

Chip Cube Interface Assembly

G1982-60050

Monitor B/W, 9 inch

G1680-63500

Trypsin Digest Methylated BSA Standard

G1990-85000

MS calibration and diagnostic chip

G4240-61001

Protein ID chip

G4240-62001

Summary of Contents for 1260 Infinity

Page 1: ...Agilent 1260 Infinity HPLC Chip Cube Interface G4240A User Manual ...

Page 2: ... this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a o...

Page 3: ...technology and explains how to install maintain and troubleshoot you HPLC Chip Cube 1 Introduction to HPLC Chip Technology 2 Introduction to the Chip Cube 3 Site Preparation 4 Installation 5 Operation 6 Maintenance Procedures 7 Consumables and Maintenance Parts Appendix A Appendix A Safety Information ...

Page 4: ...4 HPLC Chip Cube Interface User s Guide ...

Page 5: ...Introduction to the Chip Cube 19 Intended Use 20 Introduction 21 Power Switch 22 Safety Features 23 Main Assemblies 24 Principle of Operation 26 Flow Connections 29 3 Site Preparation 31 Site Requirements for the G4240A Chip Cube 32 Power Considerations 32 Bench Space 32 Environment 33 Sound Emission 34 Specifications of the G4240A HPLC Chip Cube 34 4 Installation 37 Unpacking the Agilent G4240A H...

Page 6: ...e Loading Pump and Analytical Pump 55 Injection Flush Volume 56 Injector Flush Volume and the use of Injector Programs 57 Operational Parameters 60 Setting up Chip Cube Parameters 61 Chip Valve Positions Tip 61 Time 62 Time Table 63 Instrument Actuals 63 Chip Details Screen 64 Spray Ignition 66 Conditions to avoid 69 6 Maintenance Procedures 71 Maintenance Overview 72 Replacing Capillaries 73 Clea...

Page 7: ...usion Spare Parts 85 Valve Rotor Cleaning Tools and Replacement Parts 86 8 Appendix A Safety Information 87 Safety Information 88 Power Cords 88 Operation 89 Safety Symbols 90 Chemical and Biological Safety 91 Lithium Batteries Information 92 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 93 Legal Notice 94 ...

Page 8: ...8 HPLC Chip Cube Interface User s Guide Contents ...

Page 9: ...Introduction to HPLC Chip Technology Overview 10 Chip Types 13 The MS Calibration and Diagnosis Chip Part No G4240 61001 13 The Protein ID Chip Part No G4240 62001 14 This chapter will provide you with a basic understanding of Agilent s HPLC Chip technology and an overview of the currently available chips ...

Page 10: ...d extended downtime of the complete system Quartz nano spray needles are prone to blockages and require frequent replacement With the invention of HPLC Chip technology Agilent is significantly reducing the need for user interaction and making nanospray a rugged state of the art technology The cornerstone of chip technology is the HLPC Chip a 3 dimensional structure made of sandwiched polyimide fil...

Page 11: ...from one side towards the chip while a 6 port stainless steel stator face closes in from the other side forming a pressure tight connection without any user interaction Top film with holes Biocompatible polyimide film UV Laser 2 Clean 3 Laminate 1 Ablate Bottom film with channels Finished HPLC Chip X Y Stage 4 Trim 5 Metallize CAD Drawing HV ESI contact Top film with holes Biocompatible polyimide ...

Page 12: ...ntees chemical inertness and pressure tightness with virtually no wear on the rotors and very little wear of the chip Figure 3 Rotor in rotor design of the Chip Cube valve Summary The very flexible HPLC Chip concept can combine multiple columns the valve plumbing and the nanospray emitter on one chip A rugged chip holder protects the chip and retracts the fragile tip whenever the HPLC Chip is remo...

Page 13: ...a 100 µL syringe thus infusion is possible at any time without hardware modifications by simply inserting the MS Calibration and Diagnosis Chip in the Chip Cube HPLC Valve Calibration The second function of this chip is to calibrate the Chip Cube valve Agilent 1260 Infinity HPLC Chip Cube Service Manual Valve Calibration Make sure that port 2 is connected to the nano pump and port 6 to waste Port ...

Page 14: ...application which one is preferable Agilent recommends to use Forward Flush mode since this mode of operation is less susceptible to clogging of the enrichment column Forward Flush mode Figure 4 Loading the sample in forward flush mode Port 6 of the HPLC Chip is connected to the µ well plate sampler When the valve is in the loading position the sample is transferred at a flow of 4 µL min onto the ...

Page 15: ...mn now connects to the analytical column Figure 5 Analyzing in forward flush mode As the nanoflow gradient starts the retained peptides are eluted onto the analytical column where they are further separated by reversed phase chromatography The analytical column connects directly to the nanospray emitter which is grounded using an embedded strip of noble metal that makes contact with the solvent ju...

Page 16: ...he loading solvent composition is highly aqueous the peptides are retained at the front end of the column The loading solvent is directed to waste through port 6 A filter at the back of the enrichment column retains the packing material When sample loading is finished the valve switches over to the Analyze position The front end of the enrichment column is now connected to the nano pump via port 2...

Page 17: ...hat makes contact with the solvent just before the tip The compact design of the chip significantly reduces delay volumes between enrichment and analytical columns thereby increasing efficiency compared to conventional nano LC MS systems Typical analytical flow rates are 300 nL min Preferred solvents are water acetonitrile gradients with 0 1 formic acid However most common HPLC solvents can be use...

Page 18: ...18 HPLC Chip Cube Interface User s Guide 1 Introduction to HPLC Chip Technology Chip Types More Chips Chips with different packing materials and layouts will become available in future ...

Page 19: ...ilent 1260 Infinity HPLC Chip Cube MS Interface G4240A Chip Cube User s Guide Agilent Technologies 2 Introduction to the Chip Cube Intended Use 20 Introduction 21 Main Assemblies 24 Principle of Operation 26 ...

Page 20: ...nded Use The G4240A Chip Cube is designed and tested as Controlled Office electrical equipment for laboratory use CAUTION The operator of this instrument is advised that if the equipment is used in a manner not specified in this manual the protection provided by the equipment may be impaired ...

Page 21: ... video monitor the Chip Cube Interface Assembly also referred to as Spray Chamber The HPLC Chip Cube MS Interface The Chip Cube belongs to the Agilent 1260 Infinity HPLC family It interfaces the HPLC Chip to the pump and the µ well plate sampler of the nano LC system The Chip Cube is connected to the other Agilent 1260 Infinity modules via CAN bus The main functions are Connect the HPLC Chip autom...

Page 22: ... to the MS inlet for maintenance LED illumination and a black and white CCD camera provide a convenient way of monitoring the nanospray performance on an external monitor Details about the Chip Cube Interface Assembly and operating parameters of the connected mass spectrometer can be found in the Chip Cube Interface Assembly guide Power Switch The Chip Cube power switch is located on the lower lef...

Page 23: ...ube Interface Assembly spray chamber If the interface is not recognized the Chip Cube will not move any parts and will activate the Not Ready line of the APG connector The front door interlock switch is triggered when the Chip Cube front door is opened As with the MS interlock all mechanical movements are stopped and a Not Ready condition is set Voltages in Customer Accessible Areas User accessibl...

Page 24: ... User s Guide 2 Introduction to the Chip Cube Main Assemblies Main Assemblies Figure 9 Chip Cube 5 4 1 2 6 3 1 Front door chip loading unloading button 4 Power switch 2 Status indicator 5 Front door 3 Fan grid 6 Chip loading slot ...

Page 25: ...Assemblies Figure 10 Chip Cube main assemblies 1 Attachment bolts 5 Front door 2 Strain relief of capillary guide 6 Stages assembly 3 Stator assembly with capillary cover 7 Manual clamp release knob 4 Chip feeder assembly 8 Front door chip loading unloading button 8 6 5 4 1 2 3 7 ...

Page 26: ...rs without end stops Upon power up the valves initialize As long as no HPLC Chip is loaded or the chip is in Standby position tip retracted the valves are kept in Off position This is to avoid a solvent spill inside the Chip Cube When the chip is lowered into the Operate position the valves move to the positions defined in the ChemStation method Chip Loading or Unloading Without ChemStation The fo...

Page 27: ...mp and an analytical pump for the Chip Cube Doing so is highly recommended as it ensures that the pumps switch OFF when the chip position is not in the Operate position This prevents solvent from leaking through the open stator holes into the Chip Cube Starting Nanospray Formation Once the chip is in the Operate position the pumps can be turned on When the mass spectrometer is in Operate mode high...

Page 28: ...cally the tip should be at the same level as the top counter electrode Figure 11 Illustration of good nanospray If necessary the tip position can be adjusted via ChemStation vertical axis or manually horizontal axis See Service manual for details For tips on spray optimization see Chapter 5 The key factors for spray formation and stability are the liquid flow and capillary voltage setting starting...

Page 29: ...t ports on the valve stator of the Chip Cube Four ports are currently used by the supplied HPLC Chips The attached capillaries can be left in place regardless of the chip in use Depending on the type of chip different ports are used Figure 12 Ports of the Stator Assembly The enrichment column of the Protein ID chip can be flushed in either forward flush or backflush mode For details see Modes of O...

Page 30: ...ternal flow connections is not required during standard operation Ferrules and Fittings In the Agilent G4240A Chip Cube only PEEK micro fittings with 4 mm threads are used However older Agilent 1260 Infinity Series G2226A nano pumps may require 1 32 double winged fingertight fittings see Fittings on page 84 Table 2 Solvent connection to the Chip Cube Port No Capillary Type Remarks 1 n a currently ...

Page 31: ... Cube User s Guide 3 Site Preparation Power Considerations 32 Bench Space 32 Environment 33 Sound Emission 34 Specifications of the G4240A HPLC Chip Cube 34 In this chapter you will find information about site requirements for sucessful installation and operation of the G4240A Chip Cube ...

Page 32: ...terface Assembly Video monitor Bench Space The G4240A Chip Cube requires sufficient laboratory bench space For a complete HPLC Chip MS system for the bench space needed refer to Table 3 on page 34 Only desks and benches with appropriate weight rating should be used WARNING Shock hazard or damage to your G4240A Chip Cube may result if the instrument is connected to line voltage higher than specifie...

Page 33: ...ient temperatures and relative humidity described in Table 3 on page 34 However mass spectrometers commonly have tighter operating temperature and humidity limits and therefore determine the applicable specifications CAUTION Do not store ship or use the G4240A Chip Cube under conditions where temperature fluctuations could cause condensation within the G4240A Chip Cube Condensation will damage the...

Page 34: ...91 Type Test Specifications of the G4240A HPLC Chip Cube Table 3 Physical Specifications Type Specification Comment Weight 14 kg 31 lbs Dimensions 349 298 359 mm 13 7 x 11 7 x 14 1 inch Width depth height Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 80 W active power Ambient operating temperature 5 40 C 41 104 F Ambient non operating temperatur...

Page 35: ... date of operation Communication Controller area network CAN RS 232C APG remote standard Safety features Enclosure with interlock system low voltage in maintenance areas flammability protection error detection Recommended pH range 1 0 8 5 Solvents with pH 2 3 should not contain acids which attack stainless steel The high pH range is limited due to the use fused silica capillaries Materials in use ...

Page 36: ...36 HPLC Chip Cube Interface User s Guide 3 Site Preparation Site Requirements for the G4240A Chip Cube ...

Page 37: ...ystem Setup Considerations 42 Preparation of Existing Systems 47 Chip Cube Hardware Installation 49 This chapter describes how to install the HPLC Chip MS Cube Please find instructions for converting of the mass spectrometer for use with Chip Cube and an installation guide for the G1982 60050 Chip Cube Interface Assembly spray chamber in the Chip Cube Interface Adapter guide ...

Page 38: ...aged notify the carrier and keep the shipping material for inspection by the carrier Delivery Checklist Check that all parts and materials have been delivered with the HPLC Chip Cube MS Interface G4240A Compare the shipment content with the checklist included in each instrument box Please report missing or damaged parts to your local Agilent Technologies sales and service office CAUTION If you nee...

Page 39: ...Chip MS Interface Description Quantity Part Number HPLC Chip MS Cube 1 G4240 64000 Power cord local matching the sockets of your country or region 1 Chip Cube Interface Assembly 1 G1982 60050 Monitor B W 9 inch 1 G1680 63500 Trypsin Digest Methylated BSA Standard 1 G1990 85000 MS calibration and diagnostic chip 1 G4240 61001 Protein ID chip 1 G4240 62001 ...

Page 40: ...valve fitting PEEK 2 reorder 5065 4410 pack of 6 fittings 2 plugs 5 CAN cable 1 m 1 5181 1519 6 Tweezers 1 8710 0587 7 Screwdriver Pozidriv no 1 1 8710 0899 8 Hex Key 3mm 12cm long handle 1 8710 2411 10 Chip Cube fitting M4 slotted 2 G4240 43200 11 Cube wrench 1 G4240 83800 12 Nano pump to Chip Cube 15 µm x 900 mm color orange or red 1 G4240 87300 13 Micro Well Plate Sampler to Chip Cube 25 µm x 1...

Page 41: ...llation 4 Unpacking the Agilent G4240A HPLC Chip MS Interface 19 Abrasive micro mesh for cleaning of valve rotors 1 8660 0852 20 Rotor mounting tool 1 G4240 68708 Table 6 Chip Cube Accessory Kit G4240 68705 continued Item Description Qt Part Number ...

Page 42: ...the HPLC on the left and the MS on the right side The chip cube attaches to the left side of the MS The PC can be placed virtually anywhere as it is connected to the instrument via Ethernet However since the G1323B Control Module does not work with the Chip Cube placing the computer on the same bench is more convenient for the user Depending on the available bench space the HPLC can be installed i...

Page 43: ...ation Front View Chip Cube Ion Trap MS Cooler optional Micro Well Plate Sampler Capillary Pump Nano pump Degasser Solvent Compartment NOTE In this configuration only one degasser is used so only one A solvent and one B solvent can be used for each pump NOTE For information about required bench space and load rating refer to Bench Space on page 32 ...

Page 44: ...ation Rear View GPIB or LAN to LC ChemStation APG Remote cable Remote start stop cable Chip Cube Ion Trap CAN cable Cooler optional µ Well Plate Sampler Capillary Pump Nano Pump Degasser Solvent Compartment NOTE If a G1330B ALS thermostat is part of the system it must always be installed underneath the Micro Well Plate Sampler ...

Page 45: ... Twin Stack Configuration Front View Chip Cube Cooler optional µ Well Plate Sampler Capillary Pump Degasser Solvent Cabinet Autosampler Degasser Solvent Cabinet NOTE Setting up the HPLC in two stacks requires 36 cm 14 5 inches of additional bench space For bench space and load specification see Bench Space on page 32 ...

Page 46: ...iguration Rear View CAN connections APG Remote cable Ion Trap APG Remote cable Chip Cube Cooler optional µ Well Plate Sampler Capillary Pump Degasser Solvent Cabinet Nano Pump Degasser Solvent Cabinet NOTE If a G1330B ALS thermostat is part of the system it must always be installed underneath the Micro Well Plate Sampler ...

Page 47: ... A 06 01 or higher The firmware update program and the necessary firmware files can be found on the Chemstation B 01 03 CD ROM in the Support fwupdate directory CAUTION Make sure the customer has produced current and functional backups of all user data This includes raw data files methods sequences and MS tune files Backing up user data is not covered by the Chip Cube installation NOTE Allow 36 cm...

Page 48: ...in the Chemstation Installation Manual 4 Install MS control software as described in the HPLC Chip MS interface adapter guide 5 Replace the MS ion source with the Chip Cube Interface Assembly and install the MS side cover and service door supplied with the Chip Cube Interface kit Installation details can be found in the Chip Cube Interface Manual ...

Page 49: ...ssembly The holding bolts fit into the slotted holes of the Chip Cube Interface Assembly spray chamber 8 Open the Chip Cube front door and connect the Chip Cube to any free CAN port of the Agilent 1260 Infinity stack using the supplied 1 m CAN cable see Figure 14 on page 44 9 Connect the power cable to the Chip Cube 10 Install the capillaries see Replacing Capillaries on page 73 11 Place the open ...

Page 50: ... once Figure 17 Optimal chip tip position Vertical adjustment The ideal position chip tip is slightly below the face of the MS end cap see Figure 17 Open the More Chip Cube menu by left clicking the Chip Cube icon in the ChemStation GUI and selecting Adjust Tip to move the tip up and down as required Figure 18 Adjust Tip Dialog Box HPLC Chip tip counter electrode end cap nanospray HV capillary ...

Page 51: ...umb wheel on the left bottom side of the Chip Cube Move the HPLC Chip tip further towards the counter electrode until the tip is equidistant to both end cap and counter electrode Figure 19 Position of hand wheel 15 Run the BSA checkout sample as appropriate see the Chip Cube Interface Assembly guide for details hand wheel ...

Page 52: ...52 HPLC Chip Cube Interface User s Guide 4 Installation Chip Cube Hardware Installation ...

Page 53: ...be User s Guide Agilent Technologies 5 Operation Chip Loading and Unloading 54 Configuration 55 Operational Parameters 60 Setting up Chip Cube Parameters 61 Spray Ignition 66 This chapter explains the operational parameters of the Chip Cube and provides practical hints ...

Page 54: ...lly and electrically without manual user interaction Loading a Chip 1 Place an HPLC Chip into the chip loading slot see Chip Cube on page 24 2 In Chemstation left click the Chip Cube icon or select More Chip Cube from the Instrument menu Click Standby to load the chip into standby position Figure 20 More Chip Cube Dialog box NOTE The handle of the HPLC Chip is keyed and will only fit in one orient...

Page 55: ...g with the system it is highly recommended to configure both loading pump and analytical pump for the Chip Cube When configured the pumps react to the status of the Chip Cube and turn the flow off when the chip is not in Operate spraying position This ensures that no solvent is spilled into the Chip Cube while the chip tip is retracted or when the chip is unloaded and the valve stator ports are op...

Page 56: ... Chip Cube injection valve When set the G4240A Chip Cube firmware adjusts the loading time of the enrichment column automatically when flow rates or injection volumes are changed To disable this feature enter an Injection Flush Volume of 0 µL The appropriate Injection Flush Volume can be calculated using the following equation VSeatCap VTransferCap x factor where VSeatCap delay volume of the µ WPS...

Page 57: ...un to be more precise before the sampler s start to move and draw sample and adds the found injection volumes to the Injector Flush Volume Changing the injection volume in the sequence table is possible and will be taken into account Example 1 1260 Infinity LC system with one auto sampler µWPS injection volume in method 1 µL Injection volume in sequence table for next run 5 µL Injector Flush Volum...

Page 58: ...ler with good injector program µWPS injection volume in method 1 µL CAUTION It is important to notice that injector programs will not be recognized by the Chip Cube Instead of summing up the actual volume in the sample loop of the ALS right before the injection the Chip Cube uses the default injection volume as defined in the method If there is a different injection volume defined in the sequence ...

Page 59: ...Chip Cube Interface User s Guide 59 Operation 5 Configuration Injector program Draw def vol from sample Wait 10 s Eject into seat Mainpass IFV 8 µL Resulting computed value 1 µL default inj Volume 8 µL 9 µL ...

Page 60: ... ChemStation method in accordance to the installed HPLC Chip CAUTION If the upper pressure limit of the method is higher than 150 bar the HPLC Chip may get damaged permanently if the system pressure rises above 150 bar Table 7 Typical Pump Parameters for use with Chip Cube Flow Rate Diagnostic MS Calibration Chip G4240 61001 Protein ID Chip G4240 62001 75 µm x 43 mm 5 µm C18 SB ZX Analytical pump ...

Page 61: ...clicking the Chip Cube icon and selecting Set up Chip Cube or by selecting the same command from the Instrument menu Figure 23 Chip Cube method parameters Chip Valve Positions Tip With the two drop down menus the initial positions of both valves can be set For the protein ID chip and the MS calibration chip only the inner valve is used Future chip designs may require the use of both valves ...

Page 62: ...r the inner valve The outer valve is not used with this chip see Chip Types on page 13 Time Stop Time 1 Stop time defines the duration of the analysis The run length of the complete system is determined by the instrument with the shortest run time setting because the stop signal is distributed via CAN bus APG start stop and LAN ion trap only Usually As Pump is selected which makes the analytical p...

Page 63: ...ault position for the inner valve Switching to Analysis after 2 5 min has proven to be safe for a loading flow rate of 4 µL min and sample volumes of around 1 µL with either 100 µm or 75 µm seat capillaries in the µ well plate sampler For larger sample volumes the time will need to be increased It is good practice to switch back to Enrichment before the end of the run This ensures that the enrichm...

Page 64: ...e valves used with the installed chip user comments the position of the chip and the total operation time of the chip This parameter is an important indicator of the remaining chip life time Chip Details Screen When a chip is loaded selecting Chip Details from the More Chip Cube menu see More Chip Cube Dialog box on page 54 brings up an information box with all relevant data about the loaded chip ...

Page 65: ...serial number revision max pressure column dimensions and packing material data whereas diagnostic data are updated while the chip is in operation Especially for shelf life purposes the First used and Last used dates are of interest In addition the number of injections on the chip and the Time in Operation spraying time are recorded Agilent do not specify an explicit limit to the number of injecti...

Page 66: ...oduces both evaporated ions and charged droplets The system is designed to separate and collect only evaporated ions while minimizing the charged droplets which enter the MS inlet as this approach produces the best S N Table 8 Typical instrument parameters for igniting nanospray Parameter Value Remarks nanopump 0 3 µL min 98 2 water ACN both with 0 1 formic acid loading pump 4 µL min 98 2 water AC...

Page 67: ...age setting Solvent Flow Rate Try to get a feeling for what a flow rate of 300 nL min looks like If you suspect a problem with flow stability run an HPLC Chip System Test see Agilent 1260 Infinity HPLC Chip Cube Interface G4240A Service Manual HPLC Chip System Test and fix any problems Capillary Voltage In order to initiate a nanospray a potential difference between the MS end cap see the Chip Cub...

Page 68: ...y requires no adjustment for optimal performance Any chip loaded will be moved to the correct position automatically Refer to Chapter 4 Chip Cube Hardware Installation for initial setup information CAUTION Do not operate the HPLC Chip at capillary voltages greater than 2400 V or capillary currents greater 100 nA Doing so will decrease the lifetime of the HPLC Chip ...

Page 69: ...lary voltage Turn the thumb wheel on the left bottom side of the Chip Cube Move the HPLC Chip tip further towards the counter electrode until the spray looks like Figure 26 on page 67 HPLC Chip tip counter electrode end cap nano spray HV capillary CAUTION The spray must not be angled directly into the MS entrance this will contaminate the desolvation assembly of the MS and cause high background ...

Page 70: ...HPLC Chip Cube Interface User s Guide 5 Operation Spray Ignition Figure 28 Position of the hand wheel If neither action is successful the tip may be damaged Replace the HPLC Chip with a new one hand wheel ...

Page 71: ...ies 6 Maintenance Procedures Maintenance Overview 72 Replacing Capillaries 73 Cleaning the Valve Rotors quick procedure 76 Updating the Chip Cube Firmware 79 This chapter describes the routine maintenance tasks that are recommended to maintain best performance of the HPLC Chip Cube MS Interface G4240A ...

Page 72: ... installation procedure for narrow ID capillaries Valve rotor cleaning quick procedure When system pressure test is failing Rotors stay assembled Valve rotor cleaning thorough procedure rotor replacement When quick rotor cleaning does not restore performance Cleaning the MS end cap when ion transmission is poor see also the ion trap documentation Cleaning the camera front window When the video ima...

Page 73: ...11 Parts required Fused silica PEEK capillary see Parts Preparations Unload the HPLC Chip CAUTION Only perform this procedure if the HPLC Chip Cube MS Interface G4240A is mounted on the MS or sitting flat on a table as the center of gravity shifts and the instrument will tip over without adequate support 1 Press the cover release button once When the lock opens pull the front panel down 2 Unlock t...

Page 74: ...en the thumb screw firmly The strain relief will only function properly if all 4 capillaries are in place 6 Connect the capillaries as follows to the Chip Cube valve stator For forward flush mode default port 1 not used port 2 15 µm orange to nanopump port 3 75 µm blue to infusion pump port 4 not used port 5 100 µm black to waste port 6 25 µm yellow to port 6 of the µ WPS For backflush mode port 1...

Page 75: ...r s Guide 75 Maintenance Procedures 6 Replacing Capillaries 7 Route the capillaries exactly as shown below 8 Reinstall the capillary cover Do not overtighten the cover screws 9 Flip the stages assembly up and close the front pane ...

Page 76: ...red Lint free wipe or lint free piece of cloth Parts required none Preparations Eject the HPLC Chip pumps will turn off automatically if the pumps are configured for Chip Cube Open front cover CAUTION Only perform this procedure if the HPLC Chip Cube MS Interface G4240A is mounted on the MS or sitting flat on a table as the center of gravity shifts and the instrument will tip over without adequate...

Page 77: ...rs quick procedure 1 Press the cover button quickly and open the front cover once the lock has opened 2 Open the lock screw of the latch lock and pull it out 3 Unlock the stages assembly and flip it out 4 Open the red thumb screw and remove the capillary guide tubing from the strain relief ...

Page 78: ...arefully and set it aside 6 Wipe the outer and inner rotor thoroughly with a methanol dampened lint free wipe or piece of cloth 7 Make sure no particles are left in the rotors as they will cause leaks Reinstall the stator assembly and the strain relief 8 Flip the stages assembly back into an upright position and reinstall the stages lock latch 9 Close the front cover 10 Reload the HPLC Chip ...

Page 79: ...e Chip Cube as described in the documentation 3 If the Chip Cube main board CLM was replaced perform a Valve Calibration to calibrate the positioning offsets of the valve rotor and store the values in the non volatile memory of the main board 4 If the Chip Cube main board was replaced re enter the serial number information of the module through the user interface see Agilent 1260 Infinity HPLC Chi...

Page 80: ...80 HPLC Chip Cube Interface User s Guide 6 Maintenance Procedures Updating the Chip Cube Firmware ...

Page 81: ...hip Cube MS Interface G4240A Chip Cube User s Guide Agilent Technologies 7 Consumables and Maintenance Parts HPLC Chips 82 Capillaries 83 Fittings 84 Infusion Spare Parts 85 This chapter provides information on parts for maintenance ...

Page 82: ...bed in Table 10 are available at product introduction Different packaging materials column dimension and chip layouts will become available later Table 10 Standard HPLC Chips Item Description Part Number 1 MS calibration and diagnostic chip G4240 61001 2 Protein ID Chip 40 nL trap 75um x 43mm 5um 300SB C18 ZX G4240 62001 ...

Page 83: ... 2 Micro Well Plate Sampler to Chip Cube 25 µm x 1050 mm yellow color G4240 87301 3 Syringe pump to Chip Cube Infusion 75 µm x 1000 mm blue color G4240 87303 4 Chip Cube to waste 100 µm x 1000 mm black color G4240 87302 5 Capillary pump to µ well plate sampler 50 µm x 500 mm green color G4240 87304 NOTE The 15 µm ID capillary G4240 87300 comes with two 4 mm PEEK fittings Older nano pumps with seri...

Page 84: ... Fittings Table 12 Fittings for Use with Chip Cube Item Description Part Number 1 Chip Cube fitting M4 PEEK fitting slotted G4240 43200 1 ea 2 M4 PEEK fitting used in µ well plate sampler 5065 4410 pack of 10 3 Finger tight fitting double winged nut and 1 32 ferrule 5065 4422 pack of 10 ...

Page 85: ...e with syringe pump 5183 4545 2 Microtight syringe adapter interfaces PEEK coated fused silica capillaries to syringe needle 0100 2273 3 PEEK coated fused silica capillary 75 µm x 1000 mm blue color comes with one Chip Cube fitting and one finger tight fitting 1 32 for syringe adapter G4240 87303 4 Chip Cube fitting M4 PEEK fitting slotted G4240 43200 1 ea 5 Finger tight fitting double winged nut ...

Page 86: ...acement Parts Table 14 Valve Rotor Cleaning Tools and Replacement Parts Item Description Part Number 1 Abrasive micro mesh for cleaning of valve rotors 8660 0852 2 Inner valve rotor 3 grooves G4240 23705 3 Outer valve rotor 5 grooves G4240 25206 4 Coupler for inner rotor G4240 23201 5 Coupler for outer rotor G4240 23202 6 Guide ring for inner rotor G4240 21704 ...

Page 87: ...logies Appendix A Safety Information Safety Information 88 Power Cords 88 Operation 89 Safety Symbols 90 Chemical and Biological Safety 91 Lithium Batteries Information 92 In this chapter you will find important safety information relevant to the Agilent 1260 Infinity HPLC Chip Cube Interface G4240 ...

Page 88: ...red as options with the G4240A Chip Cube The female end of all power cords is identical It plugs into the power input socket at the rear of the instrument The male end of each power cord is different and designed to match the wall socket of a particular country or region WARNING A warning alerts you to a situation that could cause physical injury or damage to the equipment Do not proceed beyond a ...

Page 89: ...ed the instrument must be made inoperative and be secured against any intended operation Any maintenance of the instrument under voltage should be avoided Do not replace components of the instruments with power turned on Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not...

Page 90: ...le 15 Safety Symbols used on Instruments and in Manuals Symbol Description Electrosensitive device The apparatus is marked with this symbol when the user should refer to the Service Manual in order to prevent harm to the operator and to protect the apparatus against damage Indicates a protected ground terminal ...

Page 91: ...n or sterilization procedures to follow if fluids escape from containers Observe all cautionary information printed on the original solution containers prior to their use Because leaks spills or loss of sample may generate aerosols observe proper safety precautions G4240A Chip Cube covers have not been designed as bioseals for aerosol or liquid containment Handle body fluids with care because they...

Page 92: ...ulated by IATA ICAO ADR RID IMDG is not allowed Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries WARNING Lithiumbatteri Eksplosionsfare ved fejlagtic handtering Udskiftning ma kun ske med batteri af samme fabrikat og type Lever det brugte batteri tilbage til leverandoren WARNING Lithiumbatteri Eksplosionsfare Ved udskiftning ...

Page 93: ...lity on all Electric and Electronic appliances from 13 Aug 2005 NOTE This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control...

Page 94: ...d right to use only this instrument Purchase of this instrument does not include any right express or implied to use any other patented product method or process or to use any other portion or component of any patented system or systems software microfluidic devices or reagents either alone or conjunction with this product unless use of such method process or other portion or component is separate...

Page 95: ......

Page 96: ... In This Book This manual gives an introduction to HPLC Chip technology and explains how to install maintain and troubleshoot you HPLC Chip Cube Introduction to HPLC Chip Technology Introduction to the Chip Cube Site Preparation Installation Operation Maintenance Procedures Consumables and Maintenance Parts Appendix A Safety Information ...

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