Agilent Technologies 1260 Infinity, User Manual

Page: 62 / 96

62 HPLC-Chip Cube Interface User’s Guide
5 Operation
Setting up Chip Cube Parameters
Protein ID Chip G4240-62001
With this chip the two positions, Enrichment and Analysis , are available. The
chip valve must be in Enrichment position at the begin of the run in order to
load the sample onto the enrichment column. The outer valve is not used with
this chip.
MS Calibration and Diagnosis Chip G4240-61001
Although this chip makes use of the inner rotor, the inner valve position does
not matter. The hydraulic design of the chip allows the use of either valve
position for the inner valve. The outer valve is not used with this chip (see
“Chip Types” on page 13).
Time
Stop Time
1 Stop time defines the duration of the analysis. The run length of the
complete system is determined by the instrument with the shortest run
time setting because the stop signal is distributed via CAN bus, APG
start/stop and LAN (ion trap only).
Usually
As Pump
is selected which makes the analytical pump the run time
controller of the system.
Post Time
Post time adds a defined time period time after the end of the analysis during
which the instrument is in post-run mode. Post run time is useful for column
re-equilibration with the initial solvent composition. Since the delay volume
from nanopump outlet to Chip Cube is 170 nL, a past time is 2.5 min is
suggested.