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TMA Grand Master 2.0 – User’s Guide

4 Operating the Main Unit

10. Pull out the piston, then hold the front cog wheel firmly and unscrew the rear cog wheel 

(marked with red) by turning it anticlockwise about two turns (360-720 degrees), and now 
you are able to remove the puncher.

Figure 11 – Puncher chuck

11. First, insert the new piston in the puncher chuck, then insert the puncher into the puncher 

chuck matching the size of the piston. Tighten the rear cog wheel, holding the front cog 
wheel firmly.

12. Insert the puncher chuck into its position and fix it with the puncher chuck locking lever. 

Ensure that the two cog wheels (motor and puncher chuck) are aligned exactly, and move the 

piston into position making attention to place the piston collar to the groove on the console.

13. Close the door of the tool change compartment.

14. In the pop-up window, select the diameter of tools you have placed in and click 

OK

.

November 5, 2013 – Rev. 1

3DHISTECH Ltd.

32(72)

Summary of Contents for TMA Grand Master 2.0

Page 1: ...GM2 0 UG EN Rev1 TMA Grand Master 2 0 User s Guide November 5 2013 Rev 1 ...

Page 2: ... Unit 21 4 1 Switching the Main Unit On or Off 21 4 2 Starting the TMA Grand Master Application 21 4 3 Main User Interface 23 4 4 Inserting and Removing Blocks 24 4 4 1 Block preparation 24 4 4 2 Cleaning block and PCR cassette preparation 25 4 4 3 Inserting a block into the main unit 27 4 4 4 Removing a block from the main unit 28 4 4 5 Removing a PCR cassette from the main unit 29 4 5 Changing t...

Page 3: ... 6 3 Replacing fuses of the main power switch 62 6 4 Contaminated Tray or Touch Sensor Surface 63 7 Troubleshooting TMA Grand Master 65 7 1 Hardware related issues 65 7 2 Software related issues 66 8 Cassette and Block Dimensions 67 9 Technical Description of the Hardware 68 9 1 Physical Dimensions and Weight 68 9 2 Accessories 68 9 3 Objective and Cameras 69 9 4 Ambient Conditions 69 9 5 Operatin...

Page 4: ... analysis of the digitized slides for example change of health status related to erroneous diagnosis from the digitized slide s that stems from or is associated with use of Product digitized slides quality of staining quality of stained slides quality of used method of staining 3DHISTECH Ltd assumes no responsibility for the functionality and fault free condition of your application programs Workf...

Page 5: ...ess information pecuniary loss or similar damage that stems from or is associated with the incorporated part of this Product that is made by Silicon Graphics Inc THE SOFTWARE IS PROVIDED AS IS AND WITHOUT WARRANTY OF ANY KIND EXPRESSED IMPLIED OR OTHERWISE INCLUDING WITHOUT LIMITATION ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE IN NO EVENT SHALL SAM LEFFLER OR SILICON GRAPH...

Page 6: ...ement control and laboratory use Part 2 101 Particular requirements for in vitro diagnostic IVD medical equipment DIN EN 55011 2009 Class B Limits and methods of measurement of radio disturbance characteristics of industrial scientific and medical ISM radio frequency equipment Conformity with Directive 98 79 EC is documented by the TMA Grand Master label This product has been tested to the require...

Page 7: ...key combination Example Cross references to other documents or sections within this document Warning Indicates that you need an additional module to use the feature there are prerequisites for the task or that special care should be taken when using certain feature of the device Important An important piece of information or a recommendation The application will work if you choose not to follow th...

Page 8: ...e tissue cores are removed from the donor and inserted into a recipient paraffin block Clone Block A duplicate triplicate quadruplicate etc of a recipient block with identical layout and core order TMA Tissue microarrays The tissue microarray consists of an array of cylindrical cores of paraffin embedded tissue that are removed from preexisting donor paraffin blocks PCR Polymerase Chain Reaction a...

Page 9: ...aster It has a robust and stable mechanical main unit It has an intuitive operation control via the keyboard and the mouse of a PC connected to the main unit The device measures block height based on which the software calculates the depth of punch drill and insertion Thus embedded cores are placed correctly into the recipient block resulting in an even surface level It makes image creation possib...

Page 10: ...cables between the main unit and the control computer Accessories box Blocks Base molds for paraffin blocks 2 1 TMA Grand Master Main Unit Front View Figure 1 TMA Grand Master front view 1 Protective housing 2 Door opener button 3 Tool change compartment door 4 Protective window 5 Trash container for paraffin flakes and discarded core material November 5 2013 Rev 1 3DHISTECH Ltd 10 72 ...

Page 11: ... the computer 2 2 Cabling For required cable connections the supplied cables must be plugged in as shown in the figure above To connect the main unit with the computer any of the three USB ports can be used Warning All cable connections must be checked for firm seating Defective cables must not be connected to any part and must be replaced with cables in proper operating condition Contact 3DHISTEC...

Page 12: ...hock 2 3 Delivery Package 1 TMA Grand Master main unit packed in a mandatory polyethylene container in a cardboard box Accessories in a metal box drill bits punchers pistons a wrench a drill bit and a puncher chuck paraffin block base molds accessories and tools for the assembly The size and the content of the box can vary according to your purchase order For further information on the basic acces...

Page 13: ...icrosoft Excel or OpenOffice on the control computer 2 4 Installing and Configuring TMA Grand Master Hardware Warning Only qualified personnel may install TMA Grand Master and perform the initial start up procedure 3DHISTECH Service technicians install and configure TMA Grand Master and hand it over to you in operating condition 2 5 Transporting the TMA Grand Master Main Unit Warning If you need t...

Page 14: ...ide plates of the main unit Figure 4 Transporting handle 4 Place the device into the designated cardboard box onto the bottom protective foam 5 Arrange protective padding on the four top corners 6 Pack the accessories into their box 7 Put the main unit and the accessory box to their designated places in the road box 8 Close the box and tape it November 5 2013 Rev 1 3DHISTECH Ltd 14 72 ...

Page 15: ...site and temporary files 500 GB if HDD is used for storing slides 1 1TB for saving database 3 2 TMA Grand Master Software Installation Important 3DHISTECH service technicians perform the installation and configuration of the TMA Grand Master software application The following installation procedure is for information purposes only follow the below steps only if you need to re install the software ...

Page 16: ...tion Disk TMA Grand Master USB driver CDM driver Camera driver VRmagic 2 Launch the TMAGrandMaster_2 0_RTM exe file that you received on a 3DHISTECH Installation Disk or downloaded in a compressed file 3 Follow the instructions in the installation wizard 4 Accept the End User License Agreement and click Next November 5 2013 Rev 1 3DHISTECH Ltd 16 72 ...

Page 17: ...r Software Application 5 Choose the installation folder for the TMA Grand Master software application Click Browse for locating a different folder than the default one then click Next to continue 6 Create the Start menu folder November 5 2013 Rev 1 3DHISTECH Ltd 17 72 ...

Page 18: ...ware Application 7 The installer automatically downloads the necessary prerequisite files Click Install to start the installation of prerequisites 8 Click Install to continue with the installation of the TMA Grand Master application November 5 2013 Rev 1 3DHISTECH Ltd 18 72 ...

Page 19: ... opened if you activate the Show User s Guide option in this window NOTE If Adobe Reader is already installed on your computer the folder containing the user documentation will be opened automatically Otherwise a pop up window is displayed offering you to download the application Click Yes and the website is opened in your browser with the download link of the product November 5 2013 Rev 1 3DHISTE...

Page 20: ...tware Application If VRmagic camera driver is installed for the first time after finalizing the installation the computer must be restarted Make sure that the Reboot now option is selected then click Finish to complete the procedure November 5 2013 Rev 1 3DHISTECH Ltd 20 72 ...

Page 21: ...ion on the control computer 2 Switch off the TMA Grand Master 3 Turn the control computer and the monitor off 4 2 Starting the TMA Grand Master Application You can start the program by double clicking onto the program icon of the desktop Warning If you start the software but the hardware is turned off a warning message appears Switch on the main unit then click Retry in the message box Warning If ...

Page 22: ...previously click Load in the Project panel that is located at the top right corner of the application window To load an existing project the following window is displayed after clicking Load in which you can narrow your search by using Date Range and or Diameter filters According to the database driven project handling each time you restart the software even without saving the current process of t...

Page 23: ...and processing method of blocks the normal view includes thumbnails of all recipient tray positions and as for donor blocks the selected tray is displayed in its full capacity The other view mode helps users to identify blocks and their statuses more easily Click at the top of recipient blocks panel and at the right end of donor blocks panel to switch to this view November 5 2013 Rev 1 3DHISTECH L...

Page 24: ...tray and if a donor block was loaded into a slot on a donor block tray its image will appear on the left section of the donor blocks panel at the bottom of the main application window At the donor panel a separator can be dragged sideways and is a used for dividing donor block panel to display TMA block thumbnails that are being processed and that are finished 4 4 Inserting and Removing Blocks 4 4...

Page 25: ...en otherwise embedded cores may be damaged after creation for example the top section of the core is sticks out of the paraffin block that can be easily broken down 4 4 2 Cleaning block and PCR cassette preparation Important Before inserting a PCR cassette into its slot ensure that the cover plate and tubes are uncontaminated One PCR cassette can be inserted into its slot before the first position...

Page 26: ...erated based on the size of the puncher in use If not every cleaning position had been used up during a previous project session you can use that block further on but make sure to manually mark the first free position After the software is informed that recipient block loading is finished it applies a layout consisting spots of the same puncher diameter on the cleaning block Important Before start...

Page 27: ...e recipient blocks 1 click the button next to the image of the specific block pairs Depending on the donor block tray that you want to rotate to inserting position click the tray ID A F 2 Alternatively if the new view is activated click Insert Block 3 on the Unused Block s panel if you want to insert a new recipient block and click Insert 4 at the donor panel to insert new donor block into the don...

Page 28: ...objective from the surface of the recipient block in order to take an accurate photo of it 11 Follow the steps 1 9 if you want to insert additional blocks into other trays If the cylinder is at the proper position it is not necessary to the click the tray ID then the button 4 4 4 Removing a block from the main unit 1 Recipient block positions are displayed in a double column on the left side of th...

Page 29: ...ttom of the PCR tubes 4 5 Changing the Drill Bit and the Puncher TMA Grand Master is delivered with drill bits and punchers of different sizes The following instructions demonstrate how to change drill bits punchers and pistons to match the hole diameter of a defined layout 1 Start the TMA Grand Master application then click the button at the main toolbar Figure 6 Main toolbar 2 According to the a...

Page 30: ...ol exchanger compartment door Figure 7 Tool compartment 4 Loosen the drill chuck locking lever marked with red that fixes the drill chuck in position and remove the drill chuck by lifting it upwards Figure 8 Removing the drill chuck November 5 2013 Rev 1 3DHISTECH Ltd 30 72 ...

Page 31: ...ards as much as you can 7 Hold the front cog wheel firmly and tighten the rear cog wheel clockwise Make sure with the help of the tool that the upper cog wheel is secured firmly 8 Insert the drill chuck into its position and fix it with the locking lever Ensure that the two cog wheels motor and drill chuck are aligned exactly 9 Loosen the puncher chuck locking lever that keeps the puncher chuck in...

Page 32: ...r into the puncher chuck matching the size of the piston Tighten the rear cog wheel holding the front cog wheel firmly 12 Insert the puncher chuck into its position and fix it with the puncher chuck locking lever Ensure that the two cog wheels motor and puncher chuck are aligned exactly and move the piston into position making attention to place the piston collar to the groove on the console 13 Cl...

Page 33: ... to twelve identical TMA blocks by taking out one tissue core sample and inserting it into each recipient block Then you can either create several slides from the TMA blocks or you can just keep one of the TMA blocks as a backup When you are filling the recipient block from up to sixty donor blocks maximum number of donor blocks the digital camera of TMA Grand Master gives you an image of each don...

Page 34: ... in its position For more information see section 11 Cassette and Block Dimensions 5 2 Working with Recipient Block Layouts 5 2 1 Creating a recipient block layout 1 To create a layout click the arrow or at the upper section of the Layout button next to the recipient block image Note that there is a icon at the bottom of this button and if you click it the layout will be deleted November 5 2013 Re...

Page 35: ...acters 4 When working with special blocks that have different sizes than displayed by default activate the Custom Block Size function and set the correct measurements 5 Select a value at the Tool Diameter section for the puncher and the drill bit sizes 6 Select the size of the Gap between the holes 7 Select the number of Columns and Rows 8 Pattern alignment can be set at the Alignment pane of the ...

Page 36: ...the mouse button pressed select the unwanted area on the layout Figure 12 Layout pattern 10 When finished creating a layout click Save 11 To create an additional layout click New 12 If you do not wish to create further layouts click Exit Important The table below shows the maximum number of holes can be drilled into a paraffin block depending on hole sizes Paraffin Block Size mm Hole Sizes mm Max ...

Page 37: ...r if you wish to delete the layout for the selected block click the Delete Layout button next to the block image 2 Select the layout you want to delete from the drop down menu and click Delete 3 If you do not wish to delete further layouts click Exit to close the window 5 3 Inserting Recipient Blocks 1 Switch on the TMA Grand Master main unit the control computer and the monitor and start the TMA ...

Page 38: ...ition 4 Select a layout for the recipient block a Click the image of the recipient block for which you want to add a layout b Select a pre defined layout from the Select Layout drop down list in the Layout Editor window then click Use this layout Note that only those layouts appear on the list which include spots of same diameter as set for tools c If the necessary layout is not yet created design...

Page 39: ... click the tab below the preview of the recipient blocks then select the desired group number that is also marked by a specific color for the easier identification e To create several blocks with different layouts repeat steps a c Important If loading a PCR cassette in the tray make sure to insert the necessary amount of cleaning blocks into recipient block positions 5 Type in the recipient block ...

Page 40: ...era also records the label area of the donor block To display the label click at the left side below the preview image If the label is upside down click Rotate 3 The Donor block ID must be entered into the text field below the donor block image After typing an ID into the Donor ID field hit Enter or click the green Enter button next to the ID text field to accept the ID NOTE When reloading a proje...

Page 41: ... area and you want to use it as identifier first activate automatic barcode reading function by clicking the Auto Barcode button at the main toolbar then after setting the proper type of barcode in the Barcode window click the Barcode button below the donor block preview image Important Barcode reading is an optional feature available for you only if it is purchased and installed on the control co...

Page 42: ... to use the PCR sampling function of TMA Grand Master simply insert the necessary amount of PCR cassettes into the designated slots The software automatically scans their presence and positions during loading block and or cassettes to donor trays The PCR button on the left of the donor block image is automatically displayed if a PCR cassette is properly inserted to its slot on the donor tray selec...

Page 43: ...er in PCR mode only and if necessary it is available for you to insert cleaning blocks into empty slots during the process without interrupting it Before starting the transfer process follow the sequence of steps below to place markers on donor blocks from which locations cores will be extracted to the selected recipient positions or PCR tubes 1 To place a PCR marker on a donor block first click t...

Page 44: ...donor block If you have not entered Recipient block ID nor Donor block ID for the selected transfer a window opens in which you must enter the IDs TMA Grand Master inserts each core into the next available spot and proceeds from left to right and from top to down order in the layout grid of the TMA block If you have activated the new view for donor blocks the block on which you have selected a cor...

Page 45: ...lable position Additional markers placed on the donor block preview image after the process is started will be displayed as on the recipient preview image Upon clicking Start they will turn to and processed accordingly You can refresh the image of the label and block by pressing the Refresh button next to the block Accordingly the process state is also displayed on the blocks represented by gear i...

Page 46: ...emove a donor block to which cores are selected to be transferred the software displays a message to warn you to place the block back to its position The lock on the right side of the donor block represents the status of the door of the device If this icon pulsates in red and green it means that there is a donor block from which all of the selected cores are extracted In this case you must click t...

Page 47: ...e selected for PCR extraction the PCR tube will contain cores from different blocks 6 To export donor block related data into a spreadsheet file after finishing the blocks click the Export button in the toolbar For more information see section 5 7 Exporting donor block data 7 If TMA Grand Master has finished the creation of TMA blocks and samples have been transferred into PCR tubes do the followi...

Page 48: ...y With the Slide overlay function you can refine the position of the digitized slide image to match the position of the block image Click Slide below the preview image of the donor block to open the Slide Overlay window In the window do the following Click Browse slide or Browse jpg or CC Connect to import the slide image Label view image helps you to select the proper slide rotate label image by ...

Page 49: ... type in the URL then click CC connect Login to CaseCenter with your user name and password Browse for the slide in the folder tree then left click on the slide to import it NOTE Make sure that Pannoramic Viewer is the default program to open a slide from CaseCenter November 5 2013 Rev 1 3DHISTECH Ltd 49 72 ...

Page 50: ...e 5 Using TMA Grand Master Flip slide image if needed by selecting one of the options Flip horizontal or Flip vertical Position slide image drag rotate zoom to correspond with the block image November 5 2013 Rev 1 3DHISTECH Ltd 50 72 ...

Page 51: ...icking the Clear reference points button You can fine tune the position of the slide by moving the Fine Rotation slider to rotate the image around the dark blue reference point You can also modify the transparency level of the JPG image layer by moving the Opacity slider This allows you to set how much the slide image should obscure the donor block image November 5 2013 Rev 1 3DHISTECH Ltd 51 72 ...

Page 52: ...with the same color as previously defined with the TMA Marker feature of the Pannoramic Viewer NOTE As marker colors and their names will be displayed in the relating cells of the Annotation color column on the export sheet to omit the usage of those colors that can be represented by their hexadecimal codes only the following colors are suggested to be used for easier identification of tissue type...

Page 53: ...ncher window In the main window confirm marker positions by clicking on them Close spots will be automatically dragged to the marker positions by the software NOTE By holding the Ctrl key while placing the marker it will not be dragged to close spots automatically If you have selected a JPG image a slider with which the opacity level can be modified is displayed on the left of the donor block imag...

Page 54: ...or each block If you want to fill other sample cores into the blocks at another time these files can be used and to which donor block data can be appended Depending on the mode you operate TMA Grand Master in either mixed mode or TMA only or PCR mode only the Export Window contains different fields and options for export settings 1 In mixed or TMA mode after the TMA blocks are created and you want...

Page 55: ...database summing up data for multiple blocks you can group them by left clicking while holding the Ctrl key on the Group items and finally click the button but if you choose to create separate for each of the selected groups click 4 Type in a name for each database into the Database name field 5 It is available for you to import an existing database to which you can append data Click Select File a...

Page 56: ...type in a file name 8 Select the desire file format XLS XLSX ODS NOTE Open file with the appropriate software for example use Excel to open file in XLS or XLSX or OpenOffice Calc to open file in ODS format 9 Click Save to create the output file or Cancel to exit the window without saving The generated spreadsheet contains three worksheets the first one is the Lookup on which there are three column...

Page 57: ... separate folders at the same location as the spreadsheet file Upon clicking the donor block ID the image of the block is displayed in a separate window he second sheet contains recipient block related data table where the core IDs are shown according to their positions in the layout November 5 2013 Rev 1 3DHISTECH Ltd 57 72 ...

Page 58: ... create a worksheet called Slides including stain types recipient block IDs and slide names In mixed mode the application creates a separate file for PCR related data The file name is extended with the PCR suffix The worksheet includes Donor ID and image with the relevant number of PCR sample If cores have been transferred to PCR tubes only the following Export Window appears after clicking the Ex...

Page 59: ...a new file 3 Click Browse to set the destination folder of the file and select the file type NOTE When opening XLS files in OpenOffice links to external images cannot be opened 4 Click Save to create the output file or Cancel to exit the window without saving 5 The created database includes three columns on one sheet including donor related data and image and the number of transferred cores Novemb...

Page 60: ...tly moistened piece of cloth You may also use detergent Wipe off oil or grease contamination traces from the surface of the main unit with a cotton swab dipped into acetone but keep away acetone ethanol from mirrors and lenses It is forbidden to clean mirrors and lenses with ethanol acetone The user should prevent any kind of fungal contamination on the opto mechanical parts of the TMA Grand Maste...

Page 61: ...compartment do the following 1 Switch off the TMA Grand Master device 2 Pull out the trash container 1 Figure 13 Trash container 3 Release lock by pulling out the lever 1 at the inner panel and move it to the marked position 2 Figure 14 Opening the service compartment door 4 Grab the handles and open the compartment door 5 Clean the compartment area 6 Close the door then lock the lever November 5 ...

Page 62: ...cable 2 The fuse compartment is located next to the main power supply switch at the back side of the TMA Grand Master 3 Release lock 1 by pushing the lever to the right so the insert 2 can be pulled out Figure 15 Releasing the lock of the fuse compartment 4 Remove insert from the compartment Figure 16 Removing the fuse compartment November 5 2013 Rev 1 3DHISTECH Ltd 62 72 ...

Page 63: ...inated Tray or Touch Sensor Surface If during initialization after switching on the TMA Grand Master device the program detects possible contamination at the surface of the F donor block tray between the positions F9 and F10 or of the touch sensor the following message appears on the screen Click Yes then follow the instructions regarding cleaning the tray surface After you finished cleaning the t...

Page 64: ...A Grand Master According to the instructions in the following message by using a dry and clean cloth gently rub the bottom surface of the touch sensor sensor is marked with red in Figure 18 Figure 18 Touch sensor November 5 2013 Rev 1 3DHISTECH Ltd 64 72 ...

Page 65: ... F10 or the touch sensor is contaminated What to do Clean the surface of the tray then the touch sensor at the specified areas For more information see section 6 4 Contaminated Tray or Touch Sensor Surface Block Image in the Application is of Poor Quality Cause Imaging errors What to do Contact 3DHISTECH support Puncher Falls Out During Punching Cause The puncher needle is detached from the collar...

Page 66: ...e computer For instance write access must be granted to C ProgramData 3DHISTECH TMAGrandMaster folder its sub folders files and in addition enable the software to modify registry logs Block image and label area not visible Cause Problem with preview camera COM driver registry entry is missing Solution Restart GTMA software application If the problem still persists try to reinstall it November 5 20...

Page 67: ...h of the block is 13 5 mm The smaller size 7 5 mm in depth means the paraffin thickness from the upper edge of the plastic cassette The height of paraffin blocks in parentheses means their total depth together with the plastic cassette Warning Do not use paraffin blocks which dimension values are higher than 24mm W x 37mm H x 10mm D otherwise the drilling and punching heads may be damaged If the p...

Page 68: ...12 44 352 Max 3 3 approximate values 9 2 Accessories Accessory Dimensions in mm Number of items Base mold for paraffin blocks 37 24 7 5 3 Drill bit Ø 0 6 Ø 1 0 Ø 1 5 Ø 2 0 2 2 2 2 Puncher Ø 0 6 Ø 1 0 Ø 1 5 Ø 2 0 3 3 3 3 Puncher chuck and Drill bit chuck N A 2 Piston Ø 0 6 Ø 1 0 Ø 1 5 Ø 2 0 1 1 1 1 For Drill bits Punchers and Pistons there are four distinctive color markings for each different size...

Page 69: ...lion Pixel 9 4 Ambient Conditions Condition Suitable Range Shipment packed Ambient temperature 40 C to 70 C Storage Ambient temperature 10 C to 40 C Maximum relative air humidity 75 at 35 C Operation Ambient temperature 10 C to 40 C Maximum relative air humidity 75 at 35 C Maximum altitude 2000 m Air pressure range 500 hPa to 1060 hPa Degree of pollution 2 November 5 2013 Rev 1 3DHISTECH Ltd 69 72...

Page 70: ... IP 20 Electrical safety in compliance with EN 61010 1 IEC 61010 1 and taking CSA UL regulations into account Over voltage category II Radio interference suppression in accordance with EN 55011 class B Noise immunity in accordance with EN 61326 2 6 2005 Main unit line input voltage 24 V DC 1 V Mains supply voltage 90 240V Mains frequency 50 60 Hz Power rating 300W November 5 2013 Rev 1 3DHISTECH L...

Page 71: ...TMA Grand Master 2 0 User s Guide Related documents Related documents Pannoramic Viewer 1 15 3 User s Guide TMA Module 1 16 User s Guide November 5 2013 Rev 1 3DHISTECH Ltd 71 72 ...

Page 72: ...4 35 36 37 38 39 43 44 45 57 58 M MRXS 8 P Pannoramic Viewer 49 52 71 PCR 8 9 25 26 33 39 42 43 44 47 Punch 9 12 24 26 28 29 31 32 33 35 40 44 46 53 65 67 68 R Recipient block 8 9 26 27 28 33 34 37 38 39 43 44 46 54 56 57 58 60 Research project 9 S Slide 4 8 28 33 44 48 49 50 51 58 T TMA 8 9 13 52 56 58 TMA Grand Master 6 9 10 11 12 13 15 16 17 18 19 21 26 29 33 36 37 42 43 44 45 47 60 61 62 63 65...

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