Page 5
ZYMO RESEARCH CORP.
Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ [email protected] ▪ www.zymoresearch.com
10. Add 200 µl
ZymoBIOMICS
™
DNA Wash Buffer 2
to the Zymo-Spin
™
IICR
Column in a Collection Tube and centrifuge at 10,000 x
g
for 1 minute.
11. Transfer the Zymo-Spin
™
IICR Column to a clean 1.5 ml microcentrifuge tube
and add 100 µl (50 µl minimum)
ZymoBIOMICS
™
DNase/RNase Free Water
directly to the column matrix and incubate for 1 minute. Centrifuge at
10,000 x
g
for 1 minute to elute the DNA
5, 6
.
12. Place a
Zymo-Spin™ III-HRC Filter
in a new Collection Tube and add 600 µl
ZymoBIOMICS™ HRC Prep Solution.
Centrifuge at 8,000 x
g
for 3 minutes.
13. Transfer the eluted DNA (Step 11) to a prepared Zymo-Spin™ III-HRC Filter in
a clean 1.5 ml microcentrifuge tube and centrifuge at exactly 16,000 x
g
for
3 minutes.
The filtered DNA is now suitable for PCR and other downstream applications.
For
Technical Assistance
:
1-888-882-9682 or E-mail
[email protected]
5
In some cases a brown-
colored pellet may form at
the bottom of the tube after
centrifugation. Avoid this
pellet when collecting the
eluted DNA.
6
If fungi or bacterial cultures
were processed; the DNA is
now suitable for all
downstream applications.
