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ZYMO RESEARCH CORP.
Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ [email protected] ▪ www.zymoresearch.com
Product Description
The ZymoBIOMICS™ DNA Miniprep Kit
is designed for purifying DNA from a wide array of sample
inputs (
e.g.
feces, soil, water, biofilms, etc.), that is immediately ready for microbiome or metagenome
analyses. The ZymoBIOMICS
™
innovative lysis system eliminates bias associated with unequal lysis
efficiencies
1
of different organisms (
e.g.
Gram-negative/positive bacteria including endospores
2
,
fungi,
protozoans, algae, etc.),
making it ideal for microbial community profiling. Unbiased mechanical lysis of tough
microbes is achieved by bead beating with the innovative ultra-high density BashingBeads
™
and validated
using the ZymoBIOMICS
™
Microbial Community Standard
3
, as shown in Figure 3. In addition, the
ZymoBIOMICS™ DNA Miniprep Kit is equipped with Zymo Research’s proprietary OneStep
™
PCR Inhibitor
Removal technology enabling PCR from the most PCR prohibitive environmental samples rich in humic and
fulvic acids, tannins, melanin, and other polyphenolic compounds. Coupling state-of-the-art lysis technology
with Zymo-Spin
™
Technology results in superior yields of ultra-pure DNA ideal for all downstream applications
including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing
4
.
Innovation. Pure & Simple.
™
Superior Yields
Zymo- Supplier
L BIOMICS
™
Q
1
P Q
2
Bias free lysis
Using ZymoBIOMICS
™
Innovative Lysis System.
1
Filter debris from lysate
using Zymo-Spin
™
IV.
Superior yields with
Zymo
Spin™ IIIC-Z
. Simply Bind,
Wash, and Elute.
Complete PCR inhibitor
removal with
Zymo Spin™
IV-HRC-Z
Superior yields.
Simply Bind, Wash, and
Elute from the Zymo-Spin
™
IIIC-Z.
Complete PCR Inhibitor
Removal
by simply filtering
the eluate with a Zymo-
Spin
™
HRC.
1
Chemical, enzymatic, and
inferior lysis matrices
(beads) lead to unrealistic
representation of organisms
in downstream metagenomic
analyses that is not
reflective of actual
abundance.
2
See endospore lysis
efficiency data in Appendix
B.
3
For more information on
the ZymoBIOMICS
™
Microbial Community
Standard (D6300) &
ZymoBIOMICS
™
Microbial
Community DNA Standard
(D6305), see Appendix C.
4
DNA is predominately
15-20 kb and amenable to
Next-Generation
Sequencing techniques
requiring high molecular
weight DNA.
Zymo Research offers a full
suite of
ZymoBIOMICS
™
Services
for reliable,
accurate microbial and
metagenomic analyses.
Services include:
Microbial
Composition Profiling, Novel
Microbe Identification, and
Customizable
Bioinformatics.
For details visit us at:
http://www.zymoresearch.com/
services/metagenomics
Or contact us at:
[email protected]
Ultra-pure DNA from Inhibitor Rich Samples
Figure 1.
The
ZymoBIOMICS
™
DNA Miniprep Kit
provides inhibitor-free DNA even when
challenged with extremely inhibitor rich samples. Real-time PCR was used to evaluate eluates
recovered using the
ZymoBIOMICS
™
DNA Miniprep Kit
, and kits from Suppliers Q
1
, P, and
Q
2
. Reaction volumes consisted of either 10% or 35% of the eluate from each kit to detect the
presence of PCR inhibitors. Each reaction contained 25 ng of
Brettanomyces
DNA. Delayed
and/or no amplification indicates PCR inhibition from inefficient inhibitor removal.
Figure 2
. The
ZymoBIOMICS
™
DNA Miniprep Kit
provides superior yields when compared to
Suppliers Q
1
, P, and Q
2
. 80 mg of feces was processed using each kit according to the
manufacturers’ recommended protocol. DNA was eluted using 100 µl ZymoBIOMICS™
DNase/RNase Free Water. 6 µl of each sample was visualized in a 1.0% (w/v)
agarose/ethidium bromide gel. Samples were processed in triplicate. L is a 1Kb ladder.
