Zeiss Standard 25 ICS, Руководство по эксплуатации

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Standard 25
ICS
B 40-029 e 01/98 3-7
Complete phase contrast is available only if the dark
phase ring in the objective covers the bright phase
stop in the condenser completely and precisely (also
see Fig. 3-7/
B ).
To achieve this, the diopter or the centering
telescope (3-6/
3 ) must be inserted in tube (3-6/ 4 )
instead of an eyepiece. Focus on the phase ring by
turning the eyelens (3-6/
1 ) of the centering
telescope via the knurled ring (3-6/ 2 ).
Equipment B
phase contrast condenser II Z 0.9 Ph1, 2, 3
(3-8/
1 )
phase contrast objectives labelled "Ph 2"
(3-6/
11 ), suitable also for transmitted-light
brightfield
Settings:
Insert phase contrast condenser II Z 0.9 Ph 1, 2,
3 (3-8/
1 ) in the condenser carrier as described in
section 2.
Screw phase contrast objective (3-6/ 11 ) into the
nosepiece and swing it into the optical beam
path.
Set the object in brightfield first by having the
turret disk (3-8/
5 ) click-stop in position "J".
Swing in the condenser front lens (3-8/ 2 ) via
lever (3-8/ 4 ).
Reduce luminous-field diaphragm (3-6/ 5 ), focus
it using the condenser, center it and open it
again (as described in section 3.2, page3-5).
Optimum image contrast in brightfield is
obtained by setting the aperture diaphragm via setting wheel (3-8/
3 ). The aperture diaphragm can be
centered using knurled wheel (3-8/ 6 ) and fixed using lever (3-8/ 7 ) which can be clamped in position.
Swing phase stop Ph 1, 2 or 3 assigned to the phase contrast objective in the beam path via turret
disk (3-8/
5 ).
Check centering of both phase stops using the centering telescope (3-6/ 3 ) in the tube, as described
above.
If the phase rings are out of center, as shown in Fig. (3-7/ A ), use knurled wheel (3-8/ 6 ) to make the
bright phase stops in the condenser exactly congruent with the dark phase ring in the objective, as
shown in Fig. (3-7/
B ).
Fig. 3-7 Centering of phase stops in phase
contrast
6
Fig. 3-8 Setting of phase contrast using Ph
condenser II Z 0.9