Standard 25
ICS
B 40-029 e 01/98
3-5
The KÖHLER illumination principle requires the following settings of the condenser, the luminous-field
diaphragm and the aperture diaphragm:
Move condenser 0.9 Z (3-3/
6
) to the upper stop position via the condenser drive, swing condenser
front lens 0.9 into the optical beam path and move aperture diaphragm in the center position via
lever (3-3/
5
).
Set (reduce) diameter of the luminous-field diaphragm (3-3/
3
) until it is visible in the field of view (3-
5/
A
). Focus the edge of the luminous-field diaphragm by slightly lowering the condenser (color-free
edge) (3-5/
B
) and center the image of the luminous-field diaphragm using the two condenser
centering screws (3-3/
7
) (3-5/
C
).
Open the luminous-field diaphragm (3-3/
3
) until it just disappears behind the edge of the field of view
(3-5/
D
).
Depending on which specimen is used, the image contrast can be adjusted via the aperture
diaphragm (3-3/
5
). For specimens featuring normal contrast, the aperture diaphragm (3-3/
5
) should
be set to approx. 2/3 of the diameter of the exit pupil of the objective.
This can be easily checked and set when the eyepiece is removed (3-5/
E
).
Finally, refocus the specimen via the coaxial fine drive (3-3/
2
).
Since field size and objective aperture change after every objective change, the setting of the
luminous-field diaphragm and the aperture diaphragm must be repeated as described above.
For all objective magnifications V
obj
10x, the condenser front lens 0.9 must be swung out of
the beam path.
Fig. 3-5
Setting of diaphragm images according to KÖHLER
Содержание Standard 25 ICS
Страница 1: ...Standard 25 ICS Transmitted Light Microscope Operating Manual...
Страница 22: ...Standard 25 ICS 2 2 B 40 029 e 01 98...
Страница 30: ...Standard 25 ICS 3 2 B 40 029 e 01 98...
Страница 46: ...Standard 25 ICS 4 2 B 40 029 e 01 98...
Страница 56: ...Standard 25 ICS 4 12 B 40 029 e 01 98...
Страница 58: ...Standard 25 ICS A 2 B 40 029 e 01 98...
Страница 61: ...Click below to find more Mipaper at www lcis com tw Mipaper at www lcis com tw...