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Standard 25

ICS

Transmitted Light

Microscope

Operating Manual

Содержание Standard 25 ICS

Страница 1: ...Standard 25 ICS Transmitted Light Microscope Operating Manual...

Страница 2: ...al is not covered by an update service Unless expressly authorized forwarding and duplication of this document and the utilization and communication of its contents are not permitted Violations will e...

Страница 3: ...hnical Data 1 10 2 START UP 2 3 2 1 Unpacking the instrument 2 3 2 2 Attachment of binocular tube 45 20 ICS or binocular phototube 35 20 ICS 2 3 2 3 Screwing in of objectives 2 3 2 Insertion of eyepie...

Страница 4: ...167 MT 3 11 3 7 2 Attachment of MC 80 DX microscope camera 35 mm film cassette 3 13 3 8 Attachment of adapters for video cameras 3 14 3 9 Insertion of 8x drawing eyepiece 3 16 4 CARE MAINTENANCE AND...

Страница 5: ...and UL directives The microscope meets the requirements of the EC directive 89 336 EC and the EMC legislation of November 9 1992 This operation manual includes information and warnings which must be...

Страница 6: ...nding on the model Always disconnect the instrument from the line before opening the instrument before changing the voltage and before changing the fuses When changing the instrument fuses make sure t...

Страница 7: ...is by repairing the instrument or by delivering an instrument free of any defect No guarantee is provided for defects caused by natural wear wearing parts in particular The instrument manufacturer is...

Страница 8: ...Standard 25 ICS VIII B 40 029 e 01 98 Overall view of the Standard 25 ICS microscope with polarization equipment...

Страница 9: ...3 1 2 Instrument description and main features 1 3 1 3 Microscope configurations and modules 1 4 1 4 Objectives 1 6 1 5 Eyepieces 1 8 1 6 Stage micrometers and eyepiece reticles 1 8 1 7 Technical Data...

Страница 10: ...40 029 e 01 98 Fig 1 1 Microscope modules 1 Eyepieces 5 Condenser carrier with condenser 2 Binocular tube 6 Mechanical stage with specimen holder 3 Stand 7 Objective 4 Coaxial coarse and fine drive 8...

Страница 11: ...es an optional camera port for photo and video documentation is also available to the user Major instrument features Sturdy and convenient stand in the pyramid design User friendly coaxial coarse and...

Страница 12: ...Standard 25 ICS 1 4 B 40 029 e 01 98 1 3 Microscope configurations and modules Fig 1 2 Standard 25 ICS microscope configurations with polarization equipment...

Страница 13: ...10x Ph 1 40x Ph 2 and 100x Ph 3 and phototube 490868 9804 490868 9904 Modules 1 1 Standard 25 ICS microscope stand with mechanical stage 75x30 R 5 position nosepiece W 0 8 including integrated illumi...

Страница 14: ...ength and not with instruments the objectives of which are marked with 160 as their mechanical tube length The exact observance of the cover slip thickness of 0 17 mm is all the more necessary the hig...

Страница 15: ...4 441031 A Plan 20x 0 45 Ph 2 0 53 0 17 441041 A Plan 40x 0 65 Ph 2 0 43 0 17 441051 A Plan 100x 1 25 Oil Ph 3 0 22 0 17 441081 Achrostigmat Transm light brightfield Achrostigmat 20x 0 45 1 6 0 17 44...

Страница 16: ...ired eyecups for the eyepieces can be ordered under the Cat No 444801 1 6 Stage micrometers and eyepiece reticles Measuring and counting using the microscope requires stage micrometers and eyepiece re...

Страница 17: ...6 mm area 12 5x12 5 mm divided in fields of 5x5 or 10x10 474068 Photo reticle MC 2 5x d 26 mm for 35 mm photography with an additional magnification of 2 5x or for large format photography with a 10x...

Страница 18: ...tion max 100 Operation Permissible ambient temperature 10 to 35 C Permissible relative humidity without condensation max 85 Altitude max 2000 m Atmospheric pressure 800 hPa to 1060 hPa 4 Operating dat...

Страница 19: ...r 20 E Pl 10x 20 Br and E Pl 10x 20 Br foc Specimen stage mechanical stage 75x30 R with ceramic surface Dimensions width x depth 160 x 140 mm Travel range width x depth 75 x 30 mm Specimen holder with...

Страница 20: ...ximum field number 20 interpupillary distance can be set between 55 and 75 mm viewing angle 35 viewing height approx 430 mm visual port tube factor 1x camera video port tube factor 1x camera video por...

Страница 21: ...2 7 Attachment of condensers 2 7 2 7 1 Attach condenser 0 9 Z 2 7 2 7 2 Attach condenser II Z 0 9 Ph1 2 3 and D 0 6 0 9 2 7 2 8 Connecting the instrument to the line 2 8 List of illustrations Fig 2 1...

Страница 22: ...Standard 25 ICS 2 2 B 40 029 e 01 98...

Страница 23: ...eaded screwdriver 2 1 4 Remove dust covers 2 1 1 from tube underside and the dovetail mount on the stand Hold the binocular tube 2 1 3 or the binocular phototube 2 1 2 in a slightly inclined position...

Страница 24: ...1 6 The slight image shift caused by the additional path through the glass is taken into account on the diopter scale by the fact that the zero point position is indicated not by the white dot W 2 4 W...

Страница 25: ...y looking through the eyepiece with reticle When the image and the eypiece reticle are in focus in the above eyepiece focus the image for the second eye via the focusing eyelens of the second eyepiece...

Страница 26: ...lternative it is also possible to use the specimen holder with sprig clip R 473448 2 6 2 or the specimen holder for one handed operation 453548 2 6 3 Here the microscope slide 2 6 7 is inserted into t...

Страница 27: ...ition The Ph 2 phase stop carrier 2 7 4 must be mounted as follows Screw locking screw 2 7 6 into the left drilled hole 2 7 7 on the underside of the condenser carrier The locking screw keeps the Ph 2...

Страница 28: ...al and make sure to inform the nearest CZ agency or CZ service agency Connect the line cable with connector 2 9 4 to the instrument socket 2 9 3 and connect the earth contact plug 2 9 5 to the line Sw...

Страница 29: ...ent of adapters for video cameras 3 14 3 9 Insertion of 8x drawing eyepiece 3 16 List of illustrations Fig 3 1 Switch on the instrument 3 3 Fig 3 2 Insertion of filters 3 3 Fig 3 3 Setting of brightfi...

Страница 30: ...Standard 25 ICS 3 2 B 40 029 e 01 98...

Страница 31: ...us field diaphragm interference wide band filter green 32x4 for contrast enhancement in b w photography of stained sections and for phase contrast CB 3 conversion filter 32x2 to generate the correct c...

Страница 32: ...0 R 3 3 8 Swing 10x objective into beam path via knurled ring 3 3 9 of the nosepiece Look throught the fixed eyepiece of the binocular tube 3 3 1 first and focus on the object via the focusing drive 3...

Страница 33: ...entering screws 3 3 7 3 5 C Open the luminous field diaphragm 3 3 3 until it just disappears behind the edge of the field of view 3 5 D Depending on which specimen is used the image contrast can be ad...

Страница 34: ...denser 0 9 Z 3 6 9 and Ph 2 phase stop carrier 3 6 7 phase contrast objectives labelled Ph 2 3 6 11 suitable also for transmitted light brightfield Settings Place unstained specimen on the mechanical...

Страница 35: ...ld first by having the turret disk 3 8 5 click stop in position J Swing in the condenser front lens 3 8 2 via lever 3 8 4 Reduce luminous field diaphragm 3 6 5 focus it using the condenser center it a...

Страница 36: ...75 1 0 Higher objective magnifications or apertures require objectives with an integrated iris aperture As described in chapter 2 the microscope is ready for operation and switched on according to sec...

Страница 37: ...enable the function to be performed the sliders must be pushed in Settings First set a suitable object in brightfield as described in section 3 2 Swing the polarizer 3 9 3 in the beam path If only the...

Страница 38: ...of the stage micrometer correspond to exactly 100 increments of the crossline micrometer as in Fig 3 10 the resulting scale value k for the used objective eyepiece reticle combination A Plan 10x 0 25...

Страница 39: ...g 3 11 2 and fix the cable release 3 11 1 if required Loosen the three hexagonal screws 3 11 6 remove the dust cover 3 11 8 from the camera tube 3 11 5 and insert the premounted unit A in the camera t...

Страница 40: ...bayonet 416010 T2 adapter for OLYMPUS OM OM bayonet 416002 T2 adapter for MINOLTA SR bayonet 416003 T2 adapter for CANON FD bayonet 416004 T2 adapter for NIKON F bayonet 416009 T2 adapter for PENTAX...

Страница 41: ...body in such a way that the contact pins firmly engage in the relevant sockets Pull out pushrod 3 12 8 completely for photomicrography When artificial light color reversal film is used the CB 3 conve...

Страница 42: ...cameras and three chip color CCD cameras to the camera tube of the Standard 25 ICS Tube Adapter Cameras 456105 60 C 2 3 1 0x 456107 60 C 2 3 0 63x 456106 60 C 1 2 0 5x 456119 60 C 1 3 3CCD 0 5x 456108...

Страница 43: ...mount thread into the video camera Insert video adapter or video zoom adapter in ENG 2 2 or ENG bayonet of the video camera and clamp it tight Insert premounted unit video camera with video adapter or...

Страница 44: ...t brightfield as described in section 3 2 Settings Remove one eyepiece 3 13 1 and insert the 8x drawing eyepiece 3 13 3 in the binocular phototube 35 20 ICS 3 13 2 instead of it Swing 8x drawing eyepi...

Страница 45: ...aintenance of the instrument 4 3 4 2 Troubleshooting 4 4 4 2 1 Changing the fuses 4 4 4 2 2 Changing the 6 V 20 W halogen lamp 4 5 4 2 3 Troubleshooting table 4 6 4 3 Table of spares wearing parts and...

Страница 46: ...Standard 25 ICS 4 2 B 40 029 e 01 98...

Страница 47: ...isten the cloths with petroleum ether Clean the front lenses of the objectives using petroleum ether but do not use alcohol When using the Standard 25 ICS in humid climatic zones proceed as follows St...

Страница 48: ...he line if required also disconnet flat plug 4 1 3 from the instrument connector 4 1 2 Check line cable and instrument plugs 4 1 3 and 4 and exchange them if required Press marked lock 4 1 5 in the di...

Страница 49: ...ble Fold down cover 4 2 2 and pull out defective halogen lamp 4 2 1 Use the protective film or a clean paper cloth to hold the new 6 V 20 W halogen lamp and insert both lamp pins carefully into the re...

Страница 50: ...see pages 3 11 and 3 13 Nosepiece with objective not switched to stop position Switch nosepiece with objective in stop position Condenser not set correctly Set condenser correctly adjustment centerin...

Страница 51: ...Air bubbles in the immersion oil Apply oil again to remove the bubbles Immersion oil at the front lens of a dry objective Clean the front lens of the dry objective see page 4 3 Corr Ring is not set to...

Страница 52: ...denser lens 0 9 correctly or switch it off see pages 3 4 3 5 Aperture diaphragm opening too small Set aperture diaphragm opening in accordance with the 2 3 rule or depending on the specimen features s...

Страница 53: ...ife of 6 V 20 W halogen lamp Replace 6 V 20 W halogen lamp see page 4 5 Incorrectly installed or broken line cable Correctly connect line cable or replace it see page 4 4 The pins of the 6 V 20 W halo...

Страница 54: ...erload protection for the integrated power supply Light filters interference wide band filter green d 32x4 Interference band filter green 546 d 32x3 CB 12 conversion filter d 32x2 CB 6 conversion filt...

Страница 55: ...25 ICS may only be performed by Carl Zeiss service staff or specially authorized personnel To ensure the optimum setting and trouble free function of your microscope even for a longer period of time w...

Страница 56: ...Standard 25 ICS 4 12 B 40 029 e 01 98...

Страница 57: ...Standard 25 ICS B 40 029 e 01 98 A 1 ANNEX List of abbreviations A 3 Physical and technical units A 4 Certification in accordance with DIN EN ISO 9001 DIN EN 46001 A 5 EC conformity declaration A 7...

Страница 58: ...Standard 25 ICS A 2 B 40 029 e 01 98...

Страница 59: ...electronically legible information e g film speed EG European Community EMV electromagnetic compatibility EN European standards ENG Electronic News Gathering E PL name of eyepiece type with aspheric...

Страница 60: ...ow blow fuse type TV television T2 Adapter standard adapter for 35 mm cameras UL Underwriters Laboratories US test authority VObj magnification of the objective VDE association of German electrotechni...

Страница 61: ...Click below to find more Mipaper at www lcis com tw Mipaper at www lcis com tw...

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