Zeiss LSM 710 Скачать руководство пользователя страница 14 | Manualshive

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Zeiss LSM 710 Скачать руководство пользователя страница 14

Carl Zeiss

LSM710

/

780 ZEN

-

12

-

Fig.17 Channels tool

5. Scanning an image

①

Select the

Channels

tool in the Left Tool Area. The

Channels

tool provides the control of

the parameters for the individual detection channels.

②

Set the Pinhole size to

1 AU

(Airy unit) for best compromise between depth discrimination

and detection efficiency.

※

Pinhole adjustment changes the Optical Slice thickness. When collecting multi-channel images, adjust

the pinholes so that each channel has the same Optical Slice thickness. This is important especially for

colocalization studies.

Adjusting pinhole size

↓

Auto Exposure

↓

Live scan

Adjusting Brightness/Contrast

↓

Setting the parameters

↓

Snap

Adjusting pinhole size

Workflow to acquire images

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Содержание LSM 710

Страница 1: ......

Страница 2: ......

Страница 3: ...equipped with a Ti Sa Laser the LSM systems are devices that belong to laser hazard class 4 These moderate and high risk classes embrace medium power and high power lasers You must take care not to ex...

Страница 4: ...the safety lock is ON Switch on the SYSTEM PC and Boot up the computer SYSTEM PC provides power to the computer This allows use of the computer and ZEN software offline Log on as LSM user Switch on th...

Страница 5: ...w a red LED The red LED indicates the power range which is critical for the lifetime of the laser 1 1 4 Switching on the Chameleon Laser If the Chameleon Coherent laser is required turn the key in fro...

Страница 6: ...in Tabs Action Tabs Tool Group Center Screen Area Fig 3 This area is for viewing Each displayed image can be displayed and or analyzed with many view options available through view tabs which can be f...

Страница 7: ...e tool header bar Another unique feature in the Imaging Software is the scalable ZEN interface This Workspace Zoom allows adjustment of the ZEN 2012 window size and fonts to the situational needs or y...

Страница 8: ...urn on it from the pull down menu Fig 7 Laser Control tool Blue Diode 405 405nm The Diode lasers is switched on automatically after Software start Argon 458 488 514nm The Argon laser has to be switche...

Страница 9: ...up menu by clicking on the Transmitted Light icon Click on the On button Set the intensity of the Halogen lamp using the slider Selecting an objective Click on the Objective icon and select the object...

Страница 10: ...ettings for track configuration For loading an existing configuration click button in the Configuration and select the appropriate one from the list box ex Single staining Ex 488 GFP FITC Alexa488 for...

Страница 11: ...below One for fastest imaging one for the best signal best compromise between both speed and best signal and the optimal setup for later linear unmixing of the dyes Click on the Apply automatically se...

Страница 12: ...ge slower image acquisition Switch track every Line Tracks are switched during scanning line by line The following settings can be changed between tracks Laser line laser intensity and channels Frame...

Страница 13: ...election of the main dichroic beam splitter MBS from the relevant list box Activation deactivation via check box of the selected channels Ch 1 2 QUASAR detectors ChS1 8 for the scanning procedure Sele...

Страница 14: ...r best compromise between depth discrimination and detection efficiency Pinhole adjustment changes the Optical Slice thickness When collecting multi channel images adjust the pinholes so that each cha...

Страница 15: ...ed images are shown in separate windows Click on the Stop button to stop the current scan procedure if necessary Select Live button for continuous fast scanning useful for finding and changing the foc...

Страница 16: ...annel during continuous scanning Click on the Stop button to stop the current scan procedure when finish the adjustment Lasers Control of the laser lines and their attenuation power Gain Master Settin...

Страница 17: ...is not bright enough it appears blue on the screen Blue zero minimum Adjusting the laser intensity Set the Pinhole to 1 Airy Unit Fig 25 Set the Gain Master high When the image is saturated reduce AO...

Страница 18: ...on Mode tool to adjust the scan speed A higher speed with averaging results in the best signal to noise ratio Scan speed 8 usually produces good results Use speed 6 or 7 for superior images Bit Depth...

Страница 19: ...s the changes made to zoom rotation and offset By clicking on the Reset All button the scan zoom is set to 1 the XY offsets are set to the zero position and the ratio angle is set to 0 Finally click o...

Страница 20: ...mages 1 Save as LSM format or czi format page 19 Merit allows to store an image together with the acquisition parameters re load your configuration from the stored image data De merit cannot load the...

Страница 21: ...the Right tool Area Fig 28 The WINDOWS Save As window appears Enter the file name and choose the appropriate image format Note the LSM 5 format is the native Carl Zeiss LSM image data format and cont...

Страница 22: ...me and save button The standard Windows File saving dialog appears Click Save to complete the export process Format File format Data Raw data Image is saved as RGB or monochrome It does not contain ov...

Страница 23: ...ed by double click and image acquisition parameters are displayed with the thumbnails Clicking the Reuse button transfers ALL acquisition parameters from the stored image data to the Microscope Hardwa...

Страница 24: ...e first and last image of the stack 1 Click on the button in the Action Button area A continuous XY scan of the set focus position will be performed 2 Use the focus drive of the microscope to focus on...

Страница 25: ...le diameter Click on the Start Experiment button to start the recording of the Z Stack When a multi dimensional acquisition tool is not selected the respective tool and its set parameters are not incl...

Страница 26: ...mode In the case of multi dimensional image Z stack time series Lambda etc you can select a single image from the stack by Dimensions or Player view controller 8 2 Split View display the individual ch...

Страница 27: ...visualized online by simply grabbing and turning the data stack with the mouse The Create image button opens a new image window and produces a 2D image of the currently used render mode to save as a 3...

Страница 28: ...8 9 Profile View display the intensity distribution of an image along a straight or curved line show the intensity values in table form and copy table to clipboard or save as text file show separate p...

Страница 29: ...llows you to enlarge reduce the zoom factor of an image Channel s are designed to switch on off channels or the display of the merged image as well as to assign color look up tables LUTs to the indivi...

Страница 30: ...ent More add a text box that displays the coordinate of a hidden dimension e g the z position in a Z Stack or the timestamp in a time series Cut region The region of a Z Stack or 4D image surrounded b...

Страница 31: ...ation in the Series tab 1 Turning axis Select from the pull down menu of Render series Turn around X Y or Start end 2 Number of Views Set the Total frames for animation 3 First angle To create 360 rot...

Страница 32: ...Carl Zeiss LSM710 780 ZEN 30 10 Operation of Light Microscope Axio Observer Z1...

Страница 33: ...il immersion objective W Water immersion objective Imm Immersions Touch the button for that objective to move an objective into the optical path Reflector Depending on the reflector turret installed s...

Страница 34: ...ch Fig 2 then wait until the fan of the Argon laser has switched off about 5min Don t switch off the main power yet After cooling finished switch off the Ar laser by the main power switch on the power...

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