Iron Man User Guide v. 1.4
14
APPENDIX C – Configuring the Light Path
:
Only turn on lasers you actually need
To turn on Argon/2 and Diode, first go to Standby and when it
says Ready, then turn it on. Argon/2 output [%] should be 50%
(do not go higher).
If you have multiple colors, you need to choose if you will
be imaging all channels in a single track or each channel in a
separate track (multi-track).
Single track is faster but can lead to bleedthrough of signal.
You can do a combination of multi-track and multi-channel –
for example, image DAPI and red together in a mult-channel
track and image green separately in its own track.
Load a premade track by clicking on the folder icon. If you
will be using the same track configuration many times, you
can save it here. Note: the loading and saving is done per
track.
This is where you can configure the mirrors and filters in
the light path and check off which channels to image per
track.
Let’s go through an example for imaging a triple labelled
sample (DAPI, FITC, Rhodamine) in a single track and
multitrack configuration.
