Iron Man User Guide v. 1.4
10
7)
Snap
– If you just want a single plane, click
snap
and save your
image. The PC does not have a lot of RAM – save and close
regularly.
8)
Z series
– If you
selected Z-stack
in the top left, you can now
control the Multidimensional Acquisition Z-stack window. You
can choose to either set the first and last positions, or you can
choose to center the stack around a given position.
-
First/Last (
most common choice)
o
Click
Live
o
Turn focus knob on microscope in one
direction until desired distance is
reached, click
set last
o
Repeat in the other direction, click
set
first
o
Either choose number of slices or step
size. The software will suggest the
optimal step size. This is for Nyquist
sampling - ~2 Z planes per confocal
volume, depends on pinhole size and
wavelengths being used.
-
Center
o
Click
Live
o
Turn focus knob to get to center plane
of sample
o
Either choose number of slices or step
size. The software will suggest the
optimal step size.
o
Click Range Select.
o
Move green line to approximate center of sample and red
lines to desired top and bottom of sample.
-
Click Start Experiment. Time remaining will be shown at the
bottom of the screen.
-
Save results.
