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OPERATION
Axio Vert.A1
Illumination and Contrast Techniques in Transmitted Light
Carl Zeiss
05/2012
431030-7044-001
79
4.11.3
Transmitted Light VAREL Contrast
VAREL (variable relief contrast) delivers relief-like images of specimens and can be used as an alternative
to phase contrast. VAREL may also be employed for curved surfaces, e.g., 96-microtiter plates, which
cannot be contrasted with phase contrast, as it is impossible to set a ring overlap.
Requirements
−
The microscope must have properly been put
into operation, as described in Section 3.
−
The microscope must be switched on.
−
Condenser with slider for VAREL contrast
−
Objective for VAREL contrast
Setting VAREL Contrast
•
Rotate the objective for VAREL contrast into the
light path.
•
Open the aperture diaphragm (Fig. 4-5/
4
) on
the condenser (Fig. 4-5/
5
) completely.
•
Using a fitting mounting frame, insert the
specimen into the retaining clips (Fig. 4-5/
3
) of
the object guide.
•
Move the VAREL position of the Ph/PlasDIC, H,
VAR slider (Fig. 4-5/
2
) into the light path until
the notch for the currently selected specimen
magnification has been found. There are three
different notches for VAREL.
•
The contrast desired can be adjusted via the
setscrew (Fig. 4-5/
1
).
You may insert the var slider in two different orientations. As a result, different relief
impressions for the various magnifications are produced, depending on the orientation
chosen. Even when the condenser is turned 90 degrees, it is possible to adapt the orientation
of the relief impression.
Microtiter plates: To ensure excellent contrasting, insert the var slider on the edge of the
cavity in such a way that the facing VAREL ring comes into operation. The right or left VAREL
ring may be used in the middle of the cavity.
Specimen field with the corresponding pupil image of the VAREL diaphragm (diaphragm appears rotated
180 degrees)
Fig. 4-6
VAREL Contrast for Microtiter Plates
Fig. 4-5
Setting VAREL Contrast
Содержание Axio Vert.A1
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