OPERATION
ZEISS
Illumination and contrast techniques
Axio Observer
158
431004-7244-001
12/2016
(3)
Setting reflected light brightfield according to KÖHLER
−
The microscope has been started properly as described in section
−
The microscope has been switched on.
•
Switch on the HAL 100 or microLED illuminator for reflected light using the RL button (Fig. 154/
10
) on
the microscope stand.
•
Adjust the light intensity by turning the control wheel (Fig. 154/
11
) on the microscope stand.
•
Place a high contrast reflected-light specimen on the microscope stage.
•
Turn the nosepiece (Fig. 154/
5
) to swivel in the 10x objective (yellow ring, see also section 5.3).
•
Use focus drive (Fig. 154/
9
) to focus on the specimen. In doing so, always focus away from the
specimen, if possible, to avoid any collision between objective and specimen.
•
Move the aperture diaphragm slide MAT setting lever (Fig. 154/
2
or
4
) into the central
position (roughly half opened or closed).
•
Use the control lever (Fig. 156/
4
) of luminous-field diaphragm slider (Fig. 154/
3
) to narrow the
luminous-field diaphragm until it becomes visible in the field of view (Fig. 155/
A
).
•
Turn the focus drive (Fig. 154/
9
) to refocus on the edge of the luminous-field diaphragm (Fig. 155/
B
)
and use the centering screws (Fig. 156/
2
,
3
) to center the luminous-field diaphragm with the edge of
the field of view (Fig. 155/
C
).
•
Then, open the luminous-field diaphragm
(Fig. 154/
3
) so that it just disappears from the
field of view (Fig. 155/
D
).
•
To set the aperture diaphragm (image contrast),
remove one eyepiece (Fig. 154/
15
) from the
binocular tube and look into the tube with your
naked eye or insert the auxiliary microscope in
place of the eyepiece.
•
Center the aperture diaphragm with the
centering screws on the aperture diaphragm
slider MAT (Fig. 154/
2
); for specimens with
medium contrast, set the control lever to about
2/3 to 4/5 of the exit pupil diameter of the
objective (Fig. 155/
E
).
In most applications, this aperture diaphragm
setting provides optimum contrast at almost ideal
resolution, and is therefore the best compromise
for the human eye.
•
Finally, reinsert the eyepiece, refocus with the
focus drive (Fig. 154/
9
) and adapt the image
brightness with control wheel (Fig. 154/
11
) to
the reflected light specimen.
Never use the aperture diaphragm to adjust image brightness. Use the illumination intensity
control wheel (Fig. 154/
11
) for this purpose!
Fig. 155
Diaphragm settings in reflected
light brightfield acc. to KÖHLER
Fig. 156
Iris diaphragm slider for reflected
light
Содержание Axio Observer Series
Страница 1: ...Axio Observer Inverted microscope Operating manual...
Страница 192: ......