Zeiss Auto Focus, Руководство по эксплуатации

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PRODUCT DESCRIPTION
Carl Zeiss Functionality Auto Focus equipment
16 425131-7044-001 06/2013
2.6 Functionality
The structured light generated by an LED in the sensor module of the Auto Focus is deflected through the
objective onto the sample and reflected from its surface. The Auto Focus automatically seeks the
maximum of the reflection signal, thereby bringing the surface of the sample into sharp focus.
Auto Focus can be operated in three different surface property modes (reflective / partially reflective /
diffuse) and with three different levels of precision (exact / balanced / fast). Further details can be found
in section 4.2 on page 23.
Changes in the focus position are registered by the sensor, with any deviations being automatically
compensated by the direct access of the controller to the Z drive of the microscope.
Samples can be used with or without a cover slip.
Only EC Epiplan objectives (with magnification of 10x or less) and EC Epiplan-Neofluar objectives
(with magnification of 10x or less) on the list of objectives can be used for samples with a cover
slip.
1 LED
2 Sensor module
3 Sensor
4 Beam splitter
5 Objective
6 Sample
Fig. 3 Function diagram of Auto Focus
Fig. 4 Auto Focus working modes
Sample
Cover slip
Standard mode of Auto Focus, focusing on the
surface of the sample Standard mode for samples with cover slip,
focusing through the cover slip on the sample
Sample