PIMT201
World Precision Instruments
19
Notice that there are two condenser annulus masks on the phase aperture frame.
(See
Fig. 26
, page 10.) One is for use with the 10X and 20X phase contrast
objectives, and the larger one is for a 40X phase contrast objective (not included).
Adjust the phase aperture frame so that the correct mask is in the light path
corresponding with the objective to be used.
Adjusting the Phase Contrast Alignment
1. Adjust the phase aperture frame so that the open filter hole is in the light path
of the condenser head. With the open filter hole in this position, the only mask in
the light path is the phase ring in the phase contrast objective.
2. Select a phase contrast objective. In this case, it will be either the 10X or the 20X.
3. Replace one of the eyepieces with the phase telescope (
Fig. 61
).
Fig. 61—Phase telescope in eyepiece
4. View the phase ring through the phase telescope.
5. Adjust the focus on the phase telescope for a sharp image of the phase ring. The
phase telescope focus is adjusted by screwing it in or out (
Fig. 62
).
Fig. 62— Focus adjustment
Содержание PIMT201
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