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Analyze the run's data

ChromScope software offers tools that quantitate the effectiveness of the methods,
instrumentation, and processes that are used to collect purified product.

Example chromatogram showing manual integration options:

To analyze the purification run's data:

1. Open the data-log file (in Current Tab or New Tab mode) for the run.

2. Choose peaks for manual integration, or use the Auto Integration function to

integrate them using an integration method.

3. Build calibration curves for the compounds of interest.

4. Use the information from the data-log file and calibration curves to determ-

ine the concentration of your compounds, and analyze the results of your
run.

Tip:

On the basis of this information, you can evaluate the effectiveness of

your settings, instrumentation, and processes, for quality control purposes,
and determine what changes, if any, are needed to achieve best results.

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August 8, 2014, 715003940 Rev. B

Prep 150 LC System

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Содержание Prep 150 LC System

Страница 1: ...Prep 150 LC System Quick Start 715003940 Revision B Copyright Waters Corporation 2014 All rights reserved ...

Страница 2: ...ii August 8 2014 715003940 Rev B ...

Страница 3: ...t consult the Waters Web site waters com Trademarks Waters and THE SCIENCE OF WHAT S POSSIBLE are registered trademarks of Waters Corporation and ChromScope is a trademark of Waters Corporation Other registered trademarks or trademarks are the sole property of their owners Customer comments Waters Technical Communications organization invites you to report any errors that you encounter in this doc...

Страница 4: ...ractice and consult your organization s safety representative for guidance FCC radiation emissions notice Changes or modifications not expressly approved by the party responsible for compliance could void the users authority to operate the equipment This device complies with Part 15 of the FCC Rules Operation is subject to the following two conditions 1 this device may not cause harmful interferen...

Страница 5: ...ning and caution advisories Operating the Prep 150 LC system When operating the Prep 150 LC system follow standard quality control QC procedures and the guidelines presented in this section Applicable symbols Symbol Definition Manufacturer Date of manufacture Authorized representative of the European Community Confirms that a manufactured product complies with all applic able European Community di...

Страница 6: ...M classification ISM Classification ISM Group 1 Class B This classification has been assigned in accordance with IEC CISPR 11 Industrial Scientific and Medical ISM instruments requirements Group 1 products apply to intentionally generated and or used conductively coupled radio frequency energy that is necessary for the internal functioning of the equipment Class B products are suitable for use in ...

Страница 7: ...ations of complex mixtures are required to establish initial work flow criteria It is not intended for use in diagnostic or biologically hazardous applications Use the system to perform various sampling tasks in these environments l Pharmaceutical development l Quality assurance and quality control l Chemical materials l Environmental sciences l Academic laboratories Documentation conventions Inst...

Страница 8: ...viii August 8 2014 715003940 Rev B Preface ...

Страница 9: ...etails and vari ations on the prescribed practices For more detailed information about each of the procedures open the online Help window in the ChromScope software and browse the list of topics there The nine procedures appear as individual chapters 1 Start the system 2 Set up your work environment 3 Verify the system and module settings 4 Select and run a method screening sample list 5 Create an...

Страница 10: ...nt If you are using the optional Prep Degasser with the 2545 QGM ensure the degasser is operating 2 Power on the ChromScope PC workstation Requirement You must wait at least 30 seconds after powering on the last module before you start the ChromScope software and Waters Instrument Console Doing so is necessary for the components to communicate properly with the software during startup 3 From the W...

Страница 11: ... prime the 2545 QGM or 2545 BGM double click the solvent pump in the ChromScope system panel and then refer to priming instructions in the 2535 2545 2555 Quaternary Gradient Module Operator s Guide Preparing the sample injector You must prepare the sample injector according to its type manual or 2707 Auto sampler To prepare the manual injector 1 With the column offline flush the injector and sampl...

Страница 12: ...lly run an instrument method using the Monitor control to set initial conditions and verify the system s operational readiness Tip You can view real time status and plots in the ChromScope System Panel and Current Run tabs to monitor flow and detector baselines For more information see these topics in the ChromScope online Help l Configure system and module settings l Determine system status l Mai...

Страница 13: ...p 150 LC system to purify product and collect fractions you can customize the system according to your pref erences These customizations become part of your user profile As such they appear whenever you log on to the system August 8 2014 715003940 Rev B 13 Set up your work environment ...

Страница 14: ...ive In the toolbar click Add Project 2 In the Configure Project dialog box enter the project s name and file location 3 Select a subfolder naming convention from the list for organizing the project s data log files Restriction After you create and save your new project you cannot change its subfolder naming convention 4 To import the files from another project into your new project select the proj...

Страница 15: ...uter tab which displays the file s name is created for each data log file that you open All opened data log files remain open Current Tab The data log file is opened in the currently selected tab which displays the file s name The data log file that was previously opened in the current tab is closed Other data log files opened in other tabs remain open Current Tab Overlay The data log file is open...

Страница 16: ...us elements of the data log file s raw data and to set preferences for how you want to display pressure units Alternative You can accept the default settings Later as you gain familiarity with the system you can change any of the settings in the Display Options panel to accommodate your preferences To set display options 1 From the Edit menu click Display Options Alternative In the toolbar click D...

Страница 17: ...u can import data log files tta from a defined location into a project s Data Files folder or subfolder This oper ation is useful for importing scouting run information used to set up the timing parameters for stacked injection sample lists Alternative Use the system to generate your own scouting run data or manually define your injection and collection timing in the run s sample list and collecti...

Страница 18: ...actions using the tabs under the System Config uration and System Status nodes in the ChromScope software Alternative To begin when using a standard Prep 150 LC manual or automated system configuration accept the software s default system configuration settings Later as you gain familiarity with the system you can change any of the settings in the System Configuration tabs to accommodate your syst...

Страница 19: ...gs for each of your solvents 3 Use the default values or make any changes you require and click Apply to save your settings 4 In the navigation tree click the System Status node 5 Click the Collection Bed Status tab to configure the collection bed layout and tube settings for the WFC III module s collection bed For more information see these topics in the ChromScope online Help l Configure system ...

Страница 20: ...ion timing settings This technique is especially useful when you are working with unknown samples or using the Sample List wizard to build stacked injection sample lists for high throughput purification Note If the scouting run data are already on the system s ChromScope work station or if you are working with known samples and do not need to fine tune the chromatography beforehand skip this step ...

Страница 21: ...the samples and methods which you select from separate lists Requirement When you create the system s instrument methods you must specify and save the settings that control flow rate detector wavelength and other critical run conditions Tip To improve peak separation try changing the solvent settings in the instrument method You can define the method s flow and composition func tions as isocratic ...

Страница 22: ...em shutdown conditions 5 Open and view the data log file generated from the scouting run 6 Analyze the data from the scouting run and use it to confirm the optimum conditions for separating your fractions Analyze the data from a scouting run For more information see these topics in the ChromScope online Help l Create sample lists for acquiring data and collecting fractions l Create sample lists us...

Страница 23: ...files and then load the updated sample list into the sample list queue Requirements l To build stacked injection sample lists that optimize throughput you must first create sequential or pooled collection methods using collection modes that are time only or time with detector signal threshold slope threshold or both l Use the Sample List Purification wizard to easily build your stacked injec tions...

Страница 24: ...wn the system 2 Edit the instrument method settings as needed and then save your settings 3 In the collection method for the run specify the settings detector signal or slope thresholds and or timed collection for directing fraction collection Tip To quickly enter the timing settings in your collection method do as follows l Load the data log file that contains the appropriate data from a scout in...

Страница 25: ...e list in the sample list queue so that you can run it in the sample list editor click Add to Queue Result The sample list appears in the sample list queue in the order that you add it You can add multiple sample lists in a particular order and run them sequentially in that order Tip You can use the up and down controls in the sample list queue to re order the sample lists in the queue To remove a...

Страница 26: ...ile performing maintenance tasks such as refilling solvents or empty ing collection vessels click Pause When ready you can restart oper ation by clicking Start l During the run if you must ensure that the remainder of a peak clears the column before the next timed injection occurs click Pause This action maintains run conditions while delaying the next timed injection When ready you can restart op...

Страница 27: ...sample list that you want to modify or click on the appropriate method tab on ChromScope s main page and edit the settings as needed For more information see these topics in the ChromScope online Help l Start and stop a run l Manually inject samples l Manually collect fractions l Optimize fraction collection l Modify a run l Configure default update settings l Enter on the fly updates l Update a r...

Страница 28: ...egration or use the Auto Integration function to integrate them using an integration method 3 Build calibration curves for the compounds of interest 4 Use the information from the data log file and calibration curves to determ ine the concentration of your compounds and analyze the results of your run Tip On the basis of this information you can evaluate the effectiveness of your settings instrume...

Страница 29: ... the ChromScope online Help l Open and view data log files l Work with integration l Work with calibration l Test the purity of peaks detected by PDA l Interpret PDA peak purity results August 8 2014 715003940 Rev B 29 Analyze the run s data ...

Страница 30: ...roup of data log files sequentially For batch processing you can also configure an option to auto matically distribute reports to a list of email recipients as a function of a sample list s batch run and report queue The reports are based on the integration method and report template settings in the sample list For details see the section Managing Data and Reporting in the ChromScope online Help T...

Страница 31: ...omScope work station 1 Open the data log file 2 Click the tab to display the chromatogram that you want captured in the report 3 From the File menu click Print Quick Report To Printer and select the printer Alternative From the toolbar click Print Quick Report To Printer to print the report on the default printer Restriction The message Printer not available appears when the printer is not connect...

Страница 32: ...e next anticipated run before the system shuts down l Whenever possible set up purification sample lists so that you complete the run and empty the sample list queue before you must empty fraction tubes refill or change solvents and change to a new sample l If using buffers thoroughly flush the system with distilled water before shut ting down the system l If using the manual injector flush and cl...

Страница 33: ...symbols Warning symbols alert you to the risk of death injury or seriously adverse physiological reactions associated with an instrument s use or misuse Heed all warnings when you install repair or operate any Waters instrument or device Waters accepts no liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing r...

Страница 34: ...sk of contacting corrosive substances Warning Risk of exposure to a toxic substance Warning Risk of personal exposure to laser radiation Warning Risk of exposure to biological agents that can pose a serious health threat Warning Risk of tipping Warning Risk of explosion Warning Risk of eye injury Specific warnings The following warnings both symbols and text can appear in the user manuals of parti...

Страница 35: ...stand the hazards associated with their handling Guidelines prescribing the proper use and handling of such materials appear in the latest edition of the National Research Council s publication Prudent Practices in the Laboratory Handling and Disposal of Chemicals Observe Good Laboratory Practice GLP at all times partic ularly when working with hazardous materials and consult the safety representa...

Страница 36: ...er Bedienungsbefugnis des Systems führen Avvertenza qualsiasi modifica o alterazione apportata a questa unità e non espressamente autorizzata dai responsabili per la conformità fa decadere il diritto all utilizzo dell apparecchiatura da parte dell utente Atencion cualquier cambio o modificación efectuado en esta unidad que no haya sido expresamente aprobado por la parte responsable del cumplimient...

Страница 37: ...rement déformés ou endom magés l Evitez d utiliser des tubes non métalliques avec du tétrahy drofurane THF ou de l acide sulfurique ou nitrique concentré l Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînent le gonflement des tuyaux non métalliques ce qui réduit considérablement leur pression de rupture Vorsicht Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere Vor...

Страница 38: ...n l El usuario deberá protegerse siempre los ojos cuando trabaje cerca de tubos de polímero sometidos a presión l Si hubiera alguna llama las proximidades l No se debe trabajar con tubos que se hayan doblado o sometido a altas presiones l Es necesario utilizar tubos de metal cuando se trabaje con tet rahidrofurano THF o ácidos nítrico o sulfúrico concentrados l Hay que tener en cuenta que el cloru...

Страница 39: ...ride 및 디메틸술폭시드 Dimethyl sulfox ide 는 비금속 튜브를 부풀려 튜브의 파열 압력을 크게 감소시킬 수 있으므로 유의하십시오 警告圧力のかかったポリマーチューブを扱うときは注意してください l 加圧されたポリマーチューブの付近では必ず保護メガネを着用してください l 近くにある火を消してください l 著しく変形したまたは折れ曲がったチューブは使用しないでください l 非金属チューブにはテトラヒドロフラン THF や高濃度の硝酸または硫酸などを流さないでください l 塩化メチレンやジメチルスルホキシドは非金属チューブの膨張を引き起こす場合がありその場合チューブは極めて低い圧力で破裂します August 8 2014 715003940 Rev B 39 Warnings that apply to all Waters instru...

Страница 40: ...tente che l eventuale utilizzo dell ap parecchiatura secondo modalità non previste dal produttore può compromettere la protezione offerta dall apparecchiatura Advertencia el usuario deberá saber que si el equipo se utiliza de forma distinta a la especificada por el fabricante las medidas de protección del equipo podrían ser insuficientes 警告使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用那麼該設備所提供的保護將被消弱警告使用者必须非常清...

Страница 41: ...ungsabdeckungen des Geräts gedruckt sind Attenzione per garantire protezione contro gli incendi sostituire i fusibili con altri dello stesso tipo aventi le caratteristiche indicate sui pannelli adiacenti alla copertura fusibili dello strumento Advertencia Para evitar incendios sustituir los fusibles por aquel los del tipo y características impresos en los paneles adyacentes a las cubiertas de los ...

Страница 42: ...li incendi sostituire i fusibili con altri dello stesso tipo aventi le caratteristiche indicate nel paragrafo Sostituzione dei fusibili del capitolo Procedure di manutenzione Advertencia Para evitar incendios sustituir los fusibles por aquel los del tipo y características indicados en la sección Sustituir fusibles 警告為了避免火災更換保險絲時應使用維護步驟章節中更換保險絲所指定之相同類型與規格的保險絲警告为了避免火灾应更换维护步骤一章的更换保险丝一节...

Страница 43: ...g symbols The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments devices and component parts are shipped Keep upright Keep dry Fragile Use no hooks August 8 2014 715003940 Rev B 43 Electrical and handling symbols ...

Страница 44: ...44 August 8 2014 715003940 Rev B A Safety Advisories ...

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