After you input the required information, the calculator displays the target method conditions. The
calculator automatically displays the L/dp of the existing method column and the target column.
Because the dwell volumes of the ACQUITY UPLC H-Class Series system are far smaller than
those of a conventional HPLC system, often a gradient hold is required.
Identical chemistries are available in UPLC and HPLC columns simplifying their selection and
allowing for a simple method transfer from the ACQUITY UPLC H-Class Series system to
standard analytical HPLC.
Finally, note that the chromatographic conditions provided by the calculator serve as a starting
point. You can further optimize these conditions based on the requirements for the separation.
See also:
Transferring methods
in the ACQUITY Console online Help.
2.9 Solvent recommendations
For more information on recommended solvents for your system, refer to
ACQUITY UPLC H-
Class, H-Class Bio, and I-Class Series Systems Solvent Considerations
on your documentation
media, or visit
April 4, 2018, 715005702 Rev. A
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